Skip to main content
. 2023 Feb 8;28(4):1648. doi: 10.3390/molecules28041648

Table A1.

EGT is evidence of the antioxidant activity of various free radicals, resulting from the determination of different sources or systems in the existing studies.

Oxidant Test System Effective Concentration (IC50) Reference
DPPH radicals 0.5 mL of a 0.4 mM DPPH ethanol solution was increased to a final volume of 2 mL by distilled water as a control mixture. 0.07 mg/mL [125]
Different volumes (1.5 to 7.5 μL) of the mushroom extract were mixed with 0.5 mL of a 0.4 mM DPPH ethanol solution and increased to a final volume of 2 mL with distilled water as a control mixture. 2.9 μL mushroom extract (17.6 mg EGT). [126]
150 μL of DPPH was added to 50 μL of PBS mixed with 50 μL EGT. 6.110 ± 0.305 mg/mL [120]
Different volumes (2 to 80 μL) of the solution were mixed with 0.5 mL of a 0.4 mM DPPH ethanol solution and increased to a final volume of 2 mL by distilled water as a control mixture. 0.008 mg/mL. [127]
0.9 mL of a 60 μM DPPH ethanol solution was increased to a final volume of 1 mL by distilled water as a control mixture. 0.6531 mg/mL EGT in the rhizome extracts [128]
ABTS radicals Different concentrations (10 μL) of the antioxidant were mixed with 1.0 mL of diluted ABTS solution (A734nm = 0.700 ± 0.020) 0.7411 mg/mL EGT in the rhizome extracts [128]
2.80 mL of 7 mM ABTS working solution was diluted to 65 mL with acetic acid buffer (pH 4). 50 μg/mL (58.58%) [129]
0.1 mL mushroom extract were mixed with 3.9 mL of diluted ABTS solution (A734nm = 0.700 ± 0.020). 15.29 μg/mg mushroom extract (96%) [68]
Peroxyl radicals Peroxyl radicals were generated by thermal homolysis of 20 mM ABAP at 35 °C in 100 mM potassium phosphate buffer, pH 7.4. 11.74 μM [51]
Hydroxyl radicals 10 μL of EGT and ascorbic acid at different concentrations, 10 μL of H2O2, 10 μL of CuCl, 10 μL of phenanthroline, and 150 μL of a mixture containing 1 mM luminol (1 mL) and carbonate buffer (pH 8.5, 17 mL) were dissolved and mixed. 70.31 ± 1.59 μg/mL [130]
Peroxynitrite radicals Peroxynitrite was generated from the decomposition of SIN−1 in the presence of 0.2 mM KMBA, 100 mM potassium phosphate buffer, pH 7.4, and 0.1 mM DTPA, at 35 °C. 15.8 μM [51]
Superoxide anions 10 μL of EGT and ascorbic acid at different concentrations, 10 μL of H2O2, 10 μL of CuCl, 10 μL of phenanthroline, and 150 μL of a mixture containing 1 mM luminol (10 mL) and carbonate buffer (pH 10.0, 20 mL) were dissolved and mixed. 160.44 ± 0.32 μg/mL [130]
Hydrogen peroxides 10 μL of EGT and ascorbic acid at different concentrations, 10 μL of 6% H2O2, and 150 μL of a mixture containing 1 mM luminol (1 mL) and carbonate buffer (pH 9.5, 17 mL) were dissolved and mixed. 11.65 ± 0.31 μg/mL [130]
500 mg/mL phosphatidylcholine liposomes were pretreated with 4.6, 22.9, and 57.3 μg/mL EGT and then treated with 10 mM alloxan. 4.6 μg/mL (67%) [112]
Lipid peroxidation 0.1 mL of 500 mM catechol as a substrate, 0.1 mL of 500 mM L-(-)-proline, 0.2 mL hemolymph, 2.7 mL of a 50 mM phosphate buffer (pH 6.8), and 0.1 mL of 100 units/mL mushroom PPO in a total volume of 3.2 mL. 122.5 μM [125]