DPPH radicals |
0.5 mL of a 0.4 mM DPPH ethanol solution was increased to a final volume of 2 mL by distilled water as a control mixture. |
0.07 mg/mL |
[125] |
Different volumes (1.5 to 7.5 μL) of the mushroom extract were mixed with 0.5 mL of a 0.4 mM DPPH ethanol solution and increased to a final volume of 2 mL with distilled water as a control mixture. |
2.9 μL mushroom extract (17.6 mg EGT). |
[126] |
150 μL of DPPH was added to 50 μL of PBS mixed with 50 μL EGT. |
6.110 ± 0.305 mg/mL |
[120] |
Different volumes (2 to 80 μL) of the solution were mixed with 0.5 mL of a 0.4 mM DPPH ethanol solution and increased to a final volume of 2 mL by distilled water as a control mixture. |
0.008 mg/mL. |
[127] |
0.9 mL of a 60 μM DPPH ethanol solution was increased to a final volume of 1 mL by distilled water as a control mixture. |
0.6531 mg/mL EGT in the rhizome extracts |
[128] |
ABTS radicals |
Different concentrations (10 μL) of the antioxidant were mixed with 1.0 mL of diluted ABTS solution (A734nm = 0.700 ± 0.020) |
0.7411 mg/mL EGT in the rhizome extracts |
[128] |
2.80 mL of 7 mM ABTS working solution was diluted to 65 mL with acetic acid buffer (pH 4). |
50 μg/mL (58.58%) |
[129] |
0.1 mL mushroom extract were mixed with 3.9 mL of diluted ABTS solution (A734nm = 0.700 ± 0.020). |
15.29 μg/mg mushroom extract (96%) |
[68] |
Peroxyl radicals |
Peroxyl radicals were generated by thermal homolysis of 20 mM ABAP at 35 °C in 100 mM potassium phosphate buffer, pH 7.4. |
11.74 μM |
[51] |
Hydroxyl radicals |
10 μL of EGT and ascorbic acid at different concentrations, 10 μL of H2O2, 10 μL of CuCl, 10 μL of phenanthroline, and 150 μL of a mixture containing 1 mM luminol (1 mL) and carbonate buffer (pH 8.5, 17 mL) were dissolved and mixed. |
70.31 ± 1.59 μg/mL |
[130] |
Peroxynitrite radicals |
Peroxynitrite was generated from the decomposition of SIN−1 in the presence of 0.2 mM KMBA, 100 mM potassium phosphate buffer, pH 7.4, and 0.1 mM DTPA, at 35 °C. |
15.8 μM |
[51] |
Superoxide anions |
10 μL of EGT and ascorbic acid at different concentrations, 10 μL of H2O2, 10 μL of CuCl, 10 μL of phenanthroline, and 150 μL of a mixture containing 1 mM luminol (10 mL) and carbonate buffer (pH 10.0, 20 mL) were dissolved and mixed. |
160.44 ± 0.32 μg/mL |
[130] |
Hydrogen peroxides |
10 μL of EGT and ascorbic acid at different concentrations, 10 μL of 6% H2O2, and 150 μL of a mixture containing 1 mM luminol (1 mL) and carbonate buffer (pH 9.5, 17 mL) were dissolved and mixed. |
11.65 ± 0.31 μg/mL |
[130] |
500 mg/mL phosphatidylcholine liposomes were pretreated with 4.6, 22.9, and 57.3 μg/mL EGT and then treated with 10 mM alloxan. |
4.6 μg/mL (67%) |
[112] |
Lipid peroxidation |
0.1 mL of 500 mM catechol as a substrate, 0.1 mL of 500 mM L-(-)-proline, 0.2 mL hemolymph, 2.7 mL of a 50 mM phosphate buffer (pH 6.8), and 0.1 mL of 100 units/mL mushroom PPO in a total volume of 3.2 mL. |
122.5 μM |
[125] |