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. Author manuscript; available in PMC: 2023 Jun 9.
Published in final edited form as: Science. 2022 Dec 8;378(6624):1111–1118. doi: 10.1126/science.abq2787

Fig. 4. Antibiotic resilience characterized by more rapid resumption of cell wall synthesis.

Fig. 4.

(A) A schematic diagram of pulse-chase experiment for tracking the regrowth of Mtb cells post antibiotic exposure using fluorescent d-amino acid incorporation (NADA and HADA). (B) Representative microscopy snapshots of the resR mutants and wild type during regrowth after 24 hours of INH (100-fold MIC) exposure (the upper panel) and no antibiotic exposure (the lower panel). Scale bar: 10 m. (C) Quantitative comparison of outgrowth between resR mutant and wild type in post antibiotic exposure group and no antibiotic exposure group, P<0.0001 for 24h and 36h of post antibiotic exposure group by double-sided Kolmogorov–Smirnov test. (D) Flow cytometry for NADA incorporation into Mtb cells after 24 hours recovery post antibiotic exposure or without drug exposure.