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. 2022 Dec 25;97(3):755–768. doi: 10.1007/s00204-022-03434-8

In silico analysis decodes transthyretin (TTR) binding and thyroid disrupting effects of per- and polyfluoroalkyl substances (PFAS)

Rupal Dharpure 1, Subrata Pramanik 1,✉, Ajay Pradhan 2,✉
PMCID: PMC9968702  PMID: 36566436

Abstract

Transthyretin (TTR) is a homo-tetramer protein involved in the transport of thyroid hormone (thyroxine; T4) in the plasma and cerebrospinal fluid. Many pollutants have been shown to bind to TTR, which could be alarming as disruption in the thyroid hormone system can lead to several physiological problems. It is also indicated that the monomerization of tetramer and destabilization of monomer can lead to amyloidogenesis. Many compounds are identified that can bind to tetramer and stabilize the tetramer leading to the inhibition of amyloid fibril formation. Other compounds are known to bind tetramer and induce amyloid fibril formation. Among the pollutants, per- and polyfluoroalkyl substances (PFAS) are known to disrupt the thyroid hormone system. The molecular mechanisms of thyroid hormone disruption could be diverse, as some are known to bind with thyroid hormone receptors, and others can bind to membrane transporters. Binding to TTR could also be one of the important pathways to alter thyroid signaling. However, the molecular interactions that drive thyroid-disrupting effects of long-chain and short-chain PFASs are not comprehensively understood at the molecular level. In this study, using a computational approach, we show that carbon chain length and functional group in PFASs are structural determinants, in which longer carbon chains of PFASs and sulfur-containing PFASs favor stronger interactions with TTR than their shorter-chained counterparts. Interestingly, short-chain PFAS also showed strong binding capacity, and the interaction energy for some was as close to the longer-chain PFAS. This suggests that short-chain PFASs are not completely safe, and their use and build-up in the environment should be carefully regulated. Of note, TTR homologs analysis suggests that thyroid-disrupting effects of PFASs could be most likely translated to TTR-like proteins and other species.

Supplementary Information

The online version contains supplementary material available at 10.1007/s00204-022-03434-8.

Keywords: Thyroid toxicity, Transthyretin, Molecular interactions, H-bond and Hydrophobic interactions, Binding energy, Hypothyroidism, Amyloidogenesis

Introduction

Per- and polyfluoroalkyl substances (PFASs) are a large family of environmentally persistent, man-made fluorinated compounds (Rickard et al. 2022). PFASs have different biological, structural, chemical, and physical properties. For instance, they can differ in molecular polarity, thermal stability, chemical stability, hydrophobicity, lipophilicity, and biological resistance to degradation (Blake et al. 2018; Buck et al. 2011; Kim et al. 2016). These properties lead to PFASs being used in a wide variety of products, such as food packaging, cooking utensils, water- and thermal-resistance materials, non-stick and protective coatings, and firefighting foams (Brendel et al. 2018; Chambers et al. 2021; Lindstrom et al. 2011). As a result, PFASs have been detected in ground and surface water, sediments, blood, and urine (Calafat et al. 2019; Cousins and Vestergren 2011; Han et al. 2018; Wang et al. 2014). Based on their carbon backbone number, PFASs are divided into long-chain (containing ≥ 6 carbons), short-chain (containing 6 < carbons < 3) and ultrashort-chain (containing ≤ 3 carbons) PFAS (Chambers et al. 2021).

Long-chain PFASs have been used for a long time and are known to be resistant to degradation. Studies have shown that long-chain PFASs, including well-known PFOS and PFOA, accumulate and remain in the environment for a long time (Neagu et al. 2021; Zeng et al. 2019). The half-life of PFOS has been determined as 4 and 40 years in human body and in the environment, respectively (Olsen et al. 2017). The main manufacturer of PFOS in the USA, 3 M, phased out its production in 2000 (Ren et al. 2016). Extensive use, production and detection of long-chain PFASs in the environment resulted in production of short-chain PFASs (Kato et al. 2011). Although these alternative plasticizers were proposed to decrease environmental and physiological burdens, they are as persistent as long-chain PFASs and more mobile in aquatic systems. As a result, short-chain PFASs are also being detected in the environment and humans (Brendel et al. 2018; Olsen et al. 2017). Besides, other compounds including perfluorotelomer alcohols have a potential to degrade to PFOA in organisms and subsequently cause humans to be additionally exposed to PFASs (Martin et al. 2005). The data on health risks associated with long- and short-chain PFASs are becoming evident. Several studies have shown that exposure to PFASs may lead to disruption of endocrine and immune systems, neurotoxicity, and defects in fetal development, damage to the liver and kidney, and carcinogenic effects (Lau et al. 2007; Olsen et al. 2017).

Thyroid gland produces thyroid hormones (thyroxine; T4 and triiodothyronine; T3) that regulate metabolism, cellular growth, immune system, reproduction, development, heat production and circadian rhythm (Brent 2012; Ikegami et al. 2019; Klecha et al. 2000; Mullur et al. 2014). T3 and T4 produced by the thyroid gland are transported to plasma by transport protein transthyretin (TTR) (Vieira and Saraiva 2014). TTR is a highly conserved soluble homo-tetramer protein that is found in several vertebrates (Power et al. 2000). TTR is synthetized by the liver and the choroid plexus of brain which are the main sources of TTR in plasma and cerebrospinal fluid (CSF), respectively (Aleshire et al. 1983; Vieira and Saraiva 2014). Along with its role as a carrier protein of thyroid hormones and retinol in plasma and CSF, TTR is also associated with nervous system physiology (Vieira and Saraiva 2014). It has been indicated that differences in TTR levels could be involved in various diseases of the nervous system, including cerebral ischemia and Alzheimer’s disease (Vieira and Saraiva 2014). Altered expression of TTR may also lead to changes in thyroid hormone level that may result in hypothyroidism/hyperthyroidism which in turn causes several health problems, such as altered metabolism, cardiac output, gastrointestinal function, neuropsychiatric and reproductive abnormalities, nervousness and palpitation, weight loss or gain, infertility, paralysis, mental problems, insomnia, etc. (Mounika et al. 2013; Williams 2008). According to American Thyroid Association, 12% of the US population is expected to develop thyroid problem during their lifetime and around 20 million people have some form of thyroid problems (Association 2019).

The increasing levels of environmental pollutants have been implicated in thyroid-related problems (Mughal et al. 2018). It is indicated that PFASs can disrupt the normal functioning of the thyroid system by altering the levels of thyroid hormones, leading to thyroid diseases (Berg et al. 2015; Dallaire et al. 2009; Liu et al. 2011; Lopez-Espinosa et al. 2012; Melzer et al. 2010; Thibodeaux et al. 2003; Webster et al. 2014; Yu et al. 2009). However, there is a lack of data on how PFASs interfere with proteins involved in the thyroid system and alter thyroid hormone levels. Computational approaches, such as molecular docking and molecular dynamics simulations, are powerful tools for determining macromolecular structure-to-function relationships. These tools could help reveal the mode of action of pollutants by predicting interactions between ligand and protein at macromolecular counterparts in full atomic detail (Hollingsworth and Dror 2018; Hospital et al. 2015). Currently, there are limited studies focusing on binding affinities of PFASs to TTR (Kar et al. 2017; Ren et al. 2016; Weiss et al. 2009). In silico studies could enhance our knowledge about the molecular interactions of PFASs with TTR and their structural attributes. The basic information obtained from in silico models could be critical in designing new PFASs and the infrastructure to develop a database for PFAS screening.

In this study, we have used in silico approach to screen long, short- and ultrashort-chain PFASs for their ability to bind human TTR and compared it to the long-chain PFASs. We show that short-chain PFASs could be as toxic as the long-chain PFASs in regulating the thyroid hormone system.

Materials and methods

PFAS dataset

A dataset comprising 15 short-chain and 16 long-chain PFASs (Ntotal = 31) was selected from literature survey (Supplemental data Table S1) (Almeida et al. 2021; Brown-Leung and Cannon 2022; Kar et al. 2017; Weiss et al. 2009). Their PubChem compound ID (CID) and 3D structures were retrieved from PubChem (https://pubchem.ncbi.nlm.nih.gov) (Kim et al. 2019). Energy minimization of each structure was done by Avagadro software using MMFF94 classical force field (Kim et al. 2016). 3D structures of a few PFASs were unavailable on PubChem and these structures were drawn using YASARA followed by energy minimization in the same software (Krieger and Vriend 2014; Land and Humble 2018). Each 3D structure file was converted into pdbqt format using OpenBabelGUI (version 3.1.1) (O'Boyle et al. 2011).

Protein preparation

The starting coordinates of human transthyretin (TTR) were taken from the X-ray crystal structure of human transthyretin (PDB ID: 1ICT, resolution 3.00 Å) (Wojtczak et al. 2001). It was prepared using mgltools from AutoDock (version 1.5.7) (Seeliger and de Groot 2010). Water molecules were deleted, polar hydrogens were added, followed by repairing the missing atoms and lastly adding the Kollman charges using mgltools (Bikadi and Hazai 2009; Huey and Morris 2008; Seeliger and de Groot 2010; Umesh et al. 2021). As T4 binds at the interface of the A–C and B–D interfaces of TTR, respectively, we used a dimer of A and C monomers for docking study (Tomar et al. 2012). Cubic grid box was set around the T4 extended to 1 Å, followed by its removal from the binding site for docking of PFASs using mgltools (Bikadi and Hazai 2009; Huey and Morris 2008; Seeliger and de Groot 2010; Umesh et al. 2021). Protein file was saved as pdbqt format.

Molecular docking of PFASs with TTR and binding energy calculation

Docking of T3, T4, and PFASs with TTR dimer was performed using AutoDock Vina (Seeliger and de Groot 2010). Performance of docking protocol was evaluated by firstly docking the natural ligands T4 and T3 and with TTR and then comparing the docking score with reference (Kar et al. 2017). Output binding energies were noted for each docking. Docked PFAS-TTR complexes were analyzed using LigPlot +- version 2.2.5 software for studying molecular interactions and identifying important interacting amino acids (Laskowski and Swindells 2011). Graphics were prepared using LigPlot +- version 2.2.5 and PyMOL (Janson and Paiardini 2021; Laskowski and Swindells 2011).

In silico generation of TTR substitutions and docking

Upon identification of the interacting amino acids with PFASs, key interacting amino acids were substituted in the TTR protein with glycine as it does not have an interacting side chain (Kim and Friesner 1997; Prento 2007). Single and multiple amino acid substitutions were performed using YASARA followed by energy minimization of the substituted TTR (Krieger and Vriend 2014; Land and Humble 2018). Docking of PFAS with substituted TTR protein was performed as described in Sect. Molecular docking of PFASs with TTR and binding energy calculation (Seeliger and de Groot 2010). LigPlot + -version 2.2 by EMBL-EBI was employed to compare interactions of wild-type TTR and substituted TTR with PFAS, respectively (Laskowski and Swindells 2011).

PFASs interspecies toxicity with TTR

Homologs of human TTR were obtained from NCBI homologous protein database (https://www.ncbi.nlm.nih.gov/homologene). A total of 12 homologs of TTR were found from 12 different vertebrate species, namely Danio rerio (Zebrafish), Xenopus tropicalis (Clawed frog), Gallus gallus (Junglefowl), Mus musculus (House mouse), Rattus norvegicus (Brown rat), Bos taurus (Cattle), Chlorocebus aethiops (Grivet), Macaca fascicularis (Macaque), Macaca mulatta (Rhesus macaque), Macaca fuscata (Japanese macaque), Pongo abelii (Sumatran orangutan), Pan troglodytes (Chimpanzee), and their sequences were retrieved from NCBI BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) (Ladunga 2009). Multiple sequence alignment was performed using EMBL-EBI Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) (Sievers and Higgins 2014). In aligned sequences, amino acids and their positions were matched with that of human TTR to see if the same amino acids are present on the same position or not in the other species, and results were visualized in Jalview software (version 2.11.2.4) (Procter et al. 2021).

Results

Long-chain PFASs showed higher toxicity propensity with TTR

The toxicity propensity of PFASs towards TTR was evaluated based on their binding energy. In this regard, binding energies of natural ligands T4 and T3 with TTR were found to be – 6.2 kcal/mol as shown in Table S1. At the dimer interface, K15 interacts with T4 and T3, respectively. Additionally, T4 also interacts with L17, E54, A108, A109, and L110 of the chain A of TTR, whereas T3 interacts with L17, T106, A108, V121, and T123 from chain A and P24, S52, and A108 from chain C (Table S1). With respect to molecular interactions, T4 forms H-bonds with L110 and A109 of chain C (amino acid AA of chain A and C are referred to as AA(A) and AA(C), respectively, throughout the text, e.g., L110 of chain C is L110(C)). On the other hand, T3 forms H-bonds with T106(A), S52(C), and T123(A), respectively. Captivatingly, both T3 and T4 formed hydrophobic interactions with various amino acids, e.g., K15(A), K15(C), and L17(C) as shown in Fig. 1 and Table S1.

Fig. 1.

Fig. 1

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Molecular interactions of natural ligands T4 and T3 with TTR. H-bonds observed between protein and ligand are indicated by green dashed lines along with bond length, while hydrophobic interactions are represented by a brown arc with spokes radiating toward the ligand. Molecular interactions between A T4 ( – 6.2 kcal/mol) and B T3 ( – 6.2 kcal/mol) with TTR are shown

In respect of PFASs, we calculated binding energy, interacting amino acids, and type of molecular interactions with TTR as explained in Table S2 and Table S3, respectively. We first aimed to identify which PFASs have stronger binding affinity with TTR than T4 and T3. To this end, binding energy analysis showed that all the 16 long-chain PFASs exerted stronger binding affinity towards TTR ranging between – 9.8 kcal/mol and – 6.7 kcal/mol (>  – 6.2 kcal/mol) compared to T3 and T4 as shown in Table S1, whereas 10 out of 15 short-chain PFASs showed a stronger binding affinity with TTR compared to T4 and T3 (Tables S1 and S3). We thus next investigated key amino acids K15, A108, A109, L110, S117, T118, and T119 from chain A and K15, A108, L110, S117, and T119 from chain C of TTR interact with long-chain PFASs. Similarly, short-chain PFASs interacted with amino acid L17, A108, A109, L110, S117, T118, and T119 from chain A and A108, A109, L110, S117, T118, and T119 from chain C of TTR (Tables S1–S3). It should the noted that K15 was frequently interacting with long-chain PFASs and not with short-chain PFASs. Besides, residues M13, E54, T106, and V121 interacted less frequently with TTR (Tables S2-S3).

Regarding the molecular interactions, we observed that H-bonds and hydrophobic interactions drive PFASs binding with TTR. Hydrophobic interactions were major contributors to the molecular interactions, followed by H-bonds (Fig. 2 and Tables S2-S3). PFASs having binding affinity stronger than T4 and T3 ( – 6.2 kcal/mol) form H-bonds predominantly with K15(A), K15(C), T106(A), T106(C), A109(C), L110(A), L110(C), S117(A), S117(C), T118(A), T118(C), T119(A), and T119(C), whereas they form hydrophobic interactions with K15(A), T106(A), T106(C), A108(A), A108(C), A109(A), L110(A), L110(C), S117(A), S117(C), T118(A), T118(C), T119(A), and T119(C) (Tables S2–S3 and Figs. 2 and 3). For example, as shown in Fig. 2, long-chain PFASs, e.g., perfluorotetradecanoic acid ( – 9.8 kcal/mol), perfluorododecanoic acid ( – 9.4 kcal/mol), perfluoroundecanoic acid ( – 9.3 kcal/mol), perfluorotributyl amine (ptba) ( – 9.2 kcal/mol), and perfluorodecane sulfonic acid ( – 9.1 kcal/mol), perfluorooctanoic acid ( – 8 kcal/mol), N-methyl perfluorooctanesulfonamido ethanol ( – 7.6 kcal/mol), and 2H-Perfluoro-2-octenoic acid ( – 7.4 kcal/mol) having stronger interactions with TTR than T4 and T3 form H-bonds with K15(C) and S117(A). Similarly, they form hydrophobic interaction through S117(A), A108(A), and A109(A) as described in Fig. 2.

Fig. 2.

Fig. 2

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Molecular interactions of long-chain PFASs with TTR. H-bonds observed between TTR and PFASs are indicated by green dashed lines along with bond length, while hydrophobic interactions are represented by brown arcs with spokes radiating toward PFASs. Molecular interactions of A perfluorotetradecanoic acid ( – 9.8 kcal/mol), B perfluorododecanoic acid ( – 9.4 kcal/mol), C perfluoroundecanoic acid ( – 9.3 kcal/mol), D 2H-perfluoro-2-octenoic acid ( – 7.4 kcal/mol), E 7H-Perfluoroheptanoic acid ( – 7.4 kcal/mol), and F 5:2 fluorotelomer alcohol ( – 6.7 kcal/mol) with TTR are shown

Fig. 3.

Fig. 3

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Molecular interactions of short-chain PFASs with TTR. H-bonds observed between protein and ligand are indicated by green dashed lines along with bond length, while hydrophobic interactions are represented by a brown arc with spokes radiating towards ligand. Molecular interactions of A perfluorohexanoic acid ( – 7.4 kcal/mol), B perfluorohexyl phosphonate ( – 7.3 kcal/mol), C perfluorohexane sulfonate ( – 7.3 kcal/mol), D perfluoroethane sulfonic acid ( – 4.9 kcal/mol), E trifluoromethane sulfonic acid ( – 4.2 kcal/mol), and F trifluoroacetic acid ( – 4.0 kcal/mol) with TTR are shown

In the case of short-chain PFASs, when we analyzed binding energy, we found a similar stronger binding affinity (i.e., H-bonds and hydrophobic interactions) like long-chain PFASs for 10 out of 15 short-chain PFASs as elucidated in Fig. 3A–C. For example, perfluorohexanoic acid ( – 7.4 kcal/mol), perfluorohexyl phosphonate ( – 7.3 kcal/mol), and perfluorohexane sulfonate ( – 7.3 kcal/mol) form H-bonds with L110(C) and S117(C) as indicated in Fig. 3A–C. On the other hand, five short-chain PFASs had shown a weaker binding affinity towards TTR compared to natural ligands T4 and T3 (<  – 6.2 kcal/mol). They had chain lengths varying between 1-C and 4-C atoms (Fig. 3D–F and Figure S7). Important interacting amino acids were found to be L17, A108, A109, L110, and T119, whereas T118 and S117 were less frequently involved. For example, perfluoroethane sulfonic acid ( – 4.9 kcal/mol), trifluoromethane sulfonic acid ( – 4.2 kcal/mol), and trifluoroacetic acid ( – 4.0 kcal/mol) formed H-bonds with T119(A) and hydrophobic interactions with L17(A), L17(C), A108(A), A108(C), A109(A), A109(C), L110(A), L110(C), S117(A), S117(C), T118(A), T119(A), and T119(C) (Fig. 3D–F and Table S3).

PFASs having a stronger binding affinity towards TTR than T4 and T3 have the potential to displace natural ligands (e.g., thyroid hormones, T4 and T3) from the binding site of TTR. As apparent from Fig. 2 and Figures S1–S6, these PFASs have carbon atoms ranging between C6 to C15, with occasional C4 to C5, whereas PFASs having a weaker binding affinity towards TTR have carbon atoms of length C1 to C4 (Fig. 3D–F and Figure S7). In summary, it can be suggested that the more the length of a chain of the PFAS, the stronger will be its binding affinity towards TTR.

K15, L110, and S117 are key interacting residues with TTR

To get a clearer picture of molecular interactions, we sought to detect key interacting residues with TTR. Comparative analysis of data revealed that K15, A108, A109, L110, and S117 are the key interacting amino acid residues of TTR that were involved in the majority of molecular interactions with PFAS (Table S4). Detailed analyses are shown in Figs. 2 and 3, Figures S1–S11, and Table S4. These residues are involved in the formation of H-bonds and hydrophobic interactions.

Multiple amino acids’ substitution confirms the key interacting amino acid of TTR for its interaction with PFASs

We next inquired whether the identified amino acid substitution with glycine alters the binding efficiency of PFASs with TTR. Critical amino acid residues from TTR involved in interacting with PFASs have been identified as mentioned before (Table S4). To understand the role of each key residue, we generated protein variants of TTR using in silico substitution of amino acid residue with glycine, including two single amino acid variants (K15G and L110G), one double amino acid variant (K15G/L110G), and one multiple amino acids variant (K15G/L110G/S117G). We then directly tested whether these substitutions in TTR could account for the resulting binding energy changes with identified six strongest and six weakest PFASs, respectively (Tables S5-S16). According to the binding energy results, the binding affinity of the selected PFASs was significantly reduced due to substitution compared to wild-type TTR as described in Table 1. Same phenomena were observed for the total number of hydrophobic interactions and H-bonds interactions, which decreased significantly (Tables S5-S16). Together, these results reveal both binding affinity and molecular interactions reduced markedly in long-chain PFASs. In contrast, the reduction for short-chain PFASs was not as marked as long-chain PFASs (Table 1).

Table 1.

Effect of amino acid substitutions on binding energies of long- and short-chain PFASs with TTR, respectively

(A) Long-chain PFASs Binding energy with wild-type TTR and variants (kcal/mol)
Wild-type K15G L110G K15G/L110G K15G/L110G/S117G
1 Perfluorotetradecanoic acid  – 9.8  – 8.8  –  8.8  – 8.2  – 7.9
2 Perfluorododecanoic acid  – 9.4  – 8.1  – 8.1  – 7.8  – 7.4
3 Perfluoroundecanoic acid  – 9.3  – 8.4  – 8.6  – 7.4  – 7.2
4 2H -Perfluoro-2-octenoic acid  – 7.4  – 7.1  – 7  – 6.4  – 6.4
5 7H-Perfluoroheptanoic acid  – 7.4  – 6.9  – 6.5  – 6.5  – 6.0
6 5:2Fluorotelomer alcohol  – 6.7  – 6.5  – 6.1  – 5.6  – 5.6
(B) Short-chain PFASs Wild-type K15G L110G K15G/L110G K15G/L110G/S117G
1 Perfluorohexanoic acid  – 7.4  – 7.0  – 6.3  – 6.4  – 6.1
2 Perfluorohexyl phosphonate  – 7.3  – 6.8  – 7.2  – 6.5  – 6.3
3 Perfluorohexane sulfonate  – 7.3  – 7.5  – 6.8  – 6.4  – 6.2
4 Perfluoroethane sulfonic acid  – 4.9  – 5.2  – 4.8  – 4.7  – 4.5
5 Trifluoromethane sulfonic acid  – 4.2  – 4.4  – 4  – 4  – 3.9
6 Trifluoroacetic acid  – 4.0  – 4.1  – 3.7  – 3.6  – 3.4
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Furthermore, single variants, K15G and L110G, were less effective in reducing the binding affinity and reducing molecular interactions as compared to double amino acid substituted variants (K15G/L110G), whereas multiple amino acid-substituted variant (K15G/L110G/S117G) has been observed to be most effective in reducing binding affinity for both long-chain and short-chain PFASs. Of note, there was a slight increase in the binding affinity of three short-chain PFASs (perfluoroethane sulfonic acid, trifluoromethane sulfonic acid, and trifluoroacetic acid) and one long-chain PFAS (N-methyl perfluorooctane sulfonamidoethanol) due to K15G and L110G substitutions, respectively. Strikingly, there are three sulfur-containing PFASs, i.e., perfluoroethane sulfonic acid, trifluoromethane sulfonic acid, and N-methylperfluorooctanesulfonamidoethanol, which showed slight increase in the binding affinity due glycine substitution. Although its structural determinant remains to be investigated, from this observation, it can be suggested that sulfur containing PFASs are more resistant and hence more toxic (Kwiatkowski et al. 2020).

TTR homologous analysis predicts PFAS toxicity in other animals

It would be extremely interesting to find out whether the toxicity of PFASs toward human TTR can be transferred to predict and access their toxicity in other animals. To probe that, we detected that TTR is a highly conserved protein during evolution among vertebrates (Prapunpoj and Leelawatwattana 2009). It is a major thyroid hormone-binding protein in vertebrates. Apart from thyroid hormone transport, TTR has several other functions (e.g., neuroprotection and promotion of neurite outgrowth in response to injury) that are the result of evolutionary changes (Hennebry et al. 2006; Liz et al. 2020). Multiple sequence alignment revealed that its primary protein structure in different vertebrates is highly conserved as can be observed in Figure S12. The amino acid residues that are identical at a particular position as of human TTR are denoted by an asterisk (*). The amino acid residue changes between different species are mainly located in the N-terminal terminal and not the binding sites of the TTR (Prapunpoj and Leelawatwattana 2009).

It can be seen in Fig. 4 that identified key residues in human TTR, e.g., K15, A108, A109, L110, S117, and T119 for interacting with PFASs are the same amino acids at the same position in all the other vertebrates species, i.e., Danio rerio (Zebrafish), Xenopus tropicalis (Clawed frog), Gallus gallus (Junglefowl), Mus musculus (House mouse), Rattus norvegicus (Brown rat), Bos taurus (Cattle), Chlorocebus aethiops (Grivet), Macaca fascicularis (Macaque), Macaca mulatta (Rhesus macaque), Macaca fuscata (Japanese macaque), Pongo abelii (Sumatran orangutan), and Pan troglodytes (Chimpanzee). Besides, we also found that invertebrates, including Caenorhabditis elegans (roundworms), Daphnia pulex (Daphnia), and Drosophila navojoa (flies) are enriched with TTR orthologs. It can be noted that A108I, S117E, and T119R substitutions were observed in Caenorhabditis elegans (Lipton et al. 2004). Likewise, A108L, L110R, and T118A substitutions were observed in Daphnia pulex (Kim et al. 2015). In contrast, Drosophila navojoa exhibits substitutions in these positions as indicated in Fig. 4 (Kockel et al. 2001; Vanderlinde et al. 2019). In summary, this observation indicates that PFASs are capable of interacting with TTR and TTR-like proteins in other animal species in a similar mechanism as they interact with human TTR.

Fig. 4.

Fig. 4

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Interspecies toxicity analysis of PFASs in 13 test species using multiple sequence alignment. Interacting residues, including K15, A108, A109, L110, S117, T118, and T119 with PFASs are conserved among different species. Column number 31 to 89 have been hidden for easy visualization of figure and shown by a vertical blue line

Discussion

A molecular and biochemical understanding of PFASs toxicity is of great importance for toxicity assessment, improving public health, environmental pollution management, and sustainable management of ecosystems (Camdzic et al. 2022; Fenton et al. 2021; Frumkin 2001; Lehman-McKeeman and Armstrong 2022; Rericha et al. 2021). Therefore, a general molecular understanding of the structural and molecular determinants that drive PFAS toxicity will help to re-design eco-friendlier and more suitable PFASs for various applications on the one hand and more effective regulation and mitigation of toxicity on the other. In our study, we examined how long- and short-chain PFASs interact with human TTR. We identified key interacting residues and interactions that drive PFASs binding with TTR and predicted PFAS toxicity in other animals. TTR is a homo-tetrameric carrier protein, which transports thyroid hormones in the plasma and cerebrospinal fluid (Rabah et al. 2019; Richardson et al. 2015). It is also involved in the transport of retinol (vitamin A) in the plasma by associating with retinol-binding protein (RBP) (Raghu and Sivakumar 2004). Besides the carrier role of TTR, many other functions have emerged including proteolysis, nerve regeneration, autophagy, and glucose homeostasis. Behavior, cognition, neuropeptide amidation, neurogenesis, nerve regeneration, and axonal growth are some of the cellular processes where TTR has an important role (Vieira and Saraiva 2014). Further, mutations in the TTR gene are implicated in the etiology of several diseases, including amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, and carpal tunnel syndrome (Bekircan-Kurt et al. 2022; Coniglio et al. 2022; Parcha et al. 2022; Takahashi et al. 2022; Yee et al. 2019). Besides, maternal exposure to PFASs can result in serum concentration of PFASs in fetuses and newborns, which leads to hypothyroidism, slow growth and development, and mental retardation as explained in Fig. 5 (Fenton et al. 2021).

Fig. 5.

Fig. 5

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PFAS toxicity in humans and other vertebrates. PFASs enter the ecosystem during their manufacturing and disposal. Humans get exposed to PFASs either through direct exposure from products or indirect environmental exposure. Environmental contamination results in toxicity threats to the biota. Insight into PFAS and human TTR interaction revealed that long and short-chain PFASs are capable of exerting toxicity by displacing THs and bonding with amino acid residues in the form of H-bonds and hydrophobic interactions. The similarity among residues and their position between human TTR and its homologs from other vertebrates indicates that the PFASs toxicity can be translated to other vertebrates and likely other species. (THs Thyroid hormones, T4 Thyroxine, T3 Triiodothyronine, PFAS per- and polyfluoroalkyl substances, TTR Transthyretin, AA Amino acid). The figure was generated using Biorender software

It is commonly known that by binding with TTR, PFASs disrupt thyroid activity and functions in both humans and experimental animals (Chain et al. 2020; Coperchini et al. 2021). Their preferential binding to proteins like TTR has been proven to cause abnormal thyroid hormone levels and thyroid diseases in monkeys, rodents, and the general human population (Cui et al. 2020; Power et al. 2000). Along with altering thyroid hormone levels, they have also been found to alter thyroid peroxidase (TPO) enzyme activity in vitro (Fenton et al. 2021). TPO plays a critical role in the generation of thyroid hormones (Godlewska and Banga 2019). However, the molecular mechanism behind the interaction of PFASs with TTR and its resultant toxicity is not comprehensively understood at the molecular level. In our study, we decode the thyroid-disrupting effects of PFASs by understanding molecular interactions between TTR protein and PFASs (Fig. 5).

According to our central hypothesis of this article, both short-chain and long-chain PFASs could be toxic, whereas long-chain PFASs are generally more toxic than short-chain analogs which need to be taken into account. 16 long-chain PFAS were analyzed for their binding affinity towards TTR and all of them had stronger binding affinity than the natural ligands, whereas out of 15 short-chain PFASs, 10 compounds were found to have stronger binding affinity than the natural ligands. It suggests that the PFASs having a stronger binding affinity towards TTR have the potential to displace natural ligands (thyroid hormones) from binding sites of TTR and cause toxicity. Besides, to strengthen our data, we compared the binding affinity of 11 Long-chain PFASs and 4 Short-chain PFASs with TTR and PPARγ (peroxisome proliferator-activated receptor gamma) as previously described (Almeida et al. 2021) (Table S17). For example, the comparison showed that perfluorododecanoic acid did not bind with PPARγ (3.5 kcal/mol), whereas it strongly binds with TTR ( – 9.4 kcal/mol). In contrast, other PFASs, such as perfluorodecane sulfonic acid and perfluorooctane sulfonic acid showed similar binding affinity toward PPARγ and TTR, respectively, as described in Table S17. Likewise, PFASs such as perfluorobutane sulfonic acid, perfluoropentanoic acid, and perfluorobutanoic acid showed a higher binding affinity with PPARγ than TTR. Taken together, these observations indicate the binding specificity of PFASs with different proteins.

With respect to the molecular interactions that drive PFASs binding with TTR, hydrophobic interactions were major contributors to binding energy in PFASs having stronger affinity, whereas H-bonds were the vital interactions in PFASs having weaker binding affinity (Tables S1–S3, Figs. 2 and 3, S1–S7). Our results are also in agreement with the earlier reports (Jensen and Warming 2015; Kar et al. 2017; Li et al. 2020; Mortensen et al. 2020; Wang et al. 2022), demonstrating that longer carbon chains of PFASs favor stronger interactions with TTR than their shorter-chained counterparts through hydrophobic interactions and thereby enhance binding affinity with TTR and eventually more toxic than the short-chain analogs. According to OECD 2020 report, long-chain PFASs are being progressively replaced with short-chain PFASs’ alternatives in US and Europe (Brendel et al. 2018; OECD 2020; Ren et al. 2016). In our study, we confirmed that long-chain PFASs and sulfur-containing PFASs are more toxic and resistant. Moreover, our study raises a new direction with respect to the toxicity of short-chain PFASs, since the toxicity of short-chain PFASs is not thoroughly studied or well described, in particular with regard to long-term effects, and there are examples of exceptions to the general picture (Brendel et al. 2018; Jensen and Warming 2015). We found that out of 15 short-chain PFASs, almost 67% of compounds had the capacity to displace thyroid hormones from TTR protein. Hence, hazards and health risks associated with short-chain PFASs need to be studied in depth (Fig. 5). Regarding interacting TTR interactions with PFASs, our data revealed that K15, A108, A109, L110, and S117 are the key interacting amino acid residues of TTR which were involved in the majority of molecular interactions with PFAS (Tables S1–S3 and Fig. 5). This observation is consistent with that of the previous studies (Kar et al. 2017; Mortensen et al. 2020). Moreover, it is prominent that H-bonds and hydrophobic interactions drive PFASs binding with TTR.

Analysis of binding energy and molecular interactions of TTR variants with PFASs confirmed that the identified amino acid residues, e.g., K15, L110, and S117 are the key residues for PFAS interactions with TTR (Table 1). In this regard, analysis of single amino acid polymorphisms/substitutions in the human population showed that various substitutions, e.g., A108T, A108Y/L110E, A109T, L110M, L110R, and T119M are associated with various diseases such as amyloidogenic transthyretin amyloidosis and peripheral neuropathy (Table S18). It is noteworthy that L110 is one of the key interacting residues with PFASs. This observation would point out the potential structure–function relationship between the TTR variant and toxicity mechanism for PFASs via protein-ligands interaction-mediated biochemical changes, and epigenetic changes, such as DNA methylation (Fenton et al. 2021; Kim et al. 2021; Xu et al. 2022).

One of the most important aspects of toxicology is managing and mitigating environmental pollution and assessing toxicity in other species, along with public health concerns. In this respect, TTR homolog analysis revealed identified key residues in human TTR, e.g., K15, A108, A109, L110, S117, and T119 for interacting with PFASs are conserved among human and other vertebrates, e.g., Pan troglodytes, Macaca fuscata, Pongo abelii, Rattus norvegicus, Danio rerio, Xenopus tropicalis. Moreover, TTR homologs are also observed in invertebrates, e.g., Caenorhabditis elegans, Daphnia pulex, and Drosophila navojoa (flies) (Fig. 4). As conserved protein sequences are known to have identical amino acid residues at analogous domains of the protein structure with similar functions (Konate et al. 2019; Schaefer et al. 2014), the protein function of TTR might be conserved among vertebrates (Pramanik et al. 2017; Sharan et al. 2005). These results strongly support that PFAS-mediated toxicity to human TTR can be used as a reference to predict toxicity to other vertebrates. However, it is crucial to consider the interspecies proteomic analysis across a large sample of species to provide further insights into the PFAS toxicity toward TTR throughout the entire phylogenetic tree of life for the sustainable management of ecosystems.

Conclusion

In summary, the results of the present study establish a discrete but comprehensive toxicity pattern of long-chain and short-chain PFASs toward TTR. Obtained results disclosed that the binding potency of a PFAS relies on its carbon chain length and functional group, in which longer carbon chains of PFASs and sulfur-containing PFASs favor stronger interactions with TTR than their shorter-chained counterparts. Moreover, these results pointed out the thyroid-disrupting effects of short-chain PFASs and their in-depth toxicity profile needs to be studied to understand the underlying biochemical processes under physiological and pathophysiological conditions. Furthermore, we identified and highlighted the significance of residues K15, A108, L110, S117, and T119 in establishing H-bonding and hydrophobic interaction networks with PFASs. Interestingly, TTR homology analysis strengthens the hypothesis that the thyroid-disrupting effects of PFASs are not only limited to humans, but to other species as well. Taken together, the data presented here facilitate molecular feature-based toxicity prediction of chemicals depending on their binding affinity with biomolecules.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 64353 KB) (62.8MB, docx)
Supplementary file2 (DOCX 73 KB) (68.8KB, docx)

Acknowledgements

AP was financed by Knowledge Foundation Sweden, Längmanska Culture Foundation, and Örebro University, Sweden. SP is supported by the SERB Start-up Research Grant (SRG) (SRG/2022/000067) and start-up grant (Reference Number 2022040816000101) from Indian Institute of Technology Guwahati. RD is supported by the University Grant Commission (UGC), New Delhi, India, for financial assistance in the form of a UGC-JRF junior research fellowship.

Abbreviations

AA

Amino acid

CSF

Cerebrospinal fluid

NCBI

National Center for Biotechnology Information

H-bond

Hydrogen bond

OECD

Organisation for Economic Co-operation and Development

PFAS

Per- and polyfluoroalkyl substances

PFOS

Perfluorooctane sulfonate

PFOA

Perfluorooctanoic acid

PPARγ

Peroxisome proliferator-activated receptor gamma

RBP

Retinol-binding protein

TH

Thyroid Hormones

TPO

Thyroid peroxidase

TTR

Transthyretin

T4

Thyroxine

T3

Triiodothyronine

A

Alanine

K

Lysine

L

Leucine

E

Glutamate

T

Threonine

V

Valine

P

Proline

S

Serine

M

Methionine

G

Glycine

Funding

Open access funding provided by Örebro University. Örebro Universitet, Stiftelsen Längmanska Kulturfonden, Knowledge Foundation, Sweden, UGC-DAE Consortium for Scientific Research, University Grants Commission, Indian Institute of Technology, Guwahati.

Data availability

All the necessary data is included in the Supplementary material. Any data that are not included will be available on request.

Declarations

Conflict of interest

The authors declare that there is no conflict of interest.

Footnotes

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Contributor Information

Subrata Pramanik, Email: subrata.pramanik@iitg.ac.in, Email: subrata.biocell@gmail.com.

Ajay Pradhan, Email: ajay.pradhan@oru.se.

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Associated Data

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Supplementary Materials

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Data Availability Statement

All the necessary data is included in the Supplementary material. Any data that are not included will be available on request.

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