Abstract
Despite producing a panoply of potential cancer-specific targets, the proteogenomic characterization of human tumors has yet to demonstrate value for precision cancer medicine. Integrative multi-omics using a machine-learning network identified master kinases responsible for effecting phenotypic hallmarks of functional glioblastoma subtypes. In subtype-matched patient-derived models, we validated PKCδ and DNA-PK as master kinases of glycolytic/plurimetabolic and proliferative/progenitor subtypes, respectively, and qualified the kinases as potent and actionable glioblastoma subtype-specific therapeutic targets. Glioblastoma subtypes were associated with clinical and radiomics features, orthogonally validated by proteomics, phospho-proteomics, metabolomics, lipidomics and acetylomics analyses, and recapitulated in pediatric glioma, breast and lung squamous cell carcinoma, including subtype specificity of PKCδ and DNA-PK activity. We developed a probabilistic classification tool that performs optimally with RNA from frozen and paraffin-embedded tissues, which can be used to evaluate the association of therapeutic response with glioblastoma subtypes and to inform patient selection in prospective clinical trials.
Subject terms: CNS cancer, Machine learning, Cancer genomics, Cancer
Iavarone and colleagues develop a computational approach called SPINKS to identify master kinases for functional subtypes of human glioblastoma defined using integrated multi-omics data, which show potential as subtype-specific therapeutic targets.
Main
The classification systems of malignant tumors have evolved in the past 15 years under the pressure of mounting molecular and genetic data and remain an active area of cancer research. The need for more accurate classifiers derives from the urgency of precision oncology and drug development targeting homogeneous tumor subsets1. Whereas genomics offers a comprehensive view of the genetic makeup of individual tumors, the integration of genomics, protein profiling and post-translational regulation delivers a deeper understanding of tumor biology and recognizes similarity patterns within individual tumor types, and possibly across multiple types of tumors that can fine-tune targeted therapeutics2.
Cancer proteomics consortia have recently provided proteogenomic data and the initial framework for analysis of the proteomic platforms and integration with genomic data3,4.
Here, we reconstructed four functional subtypes of glioblastoma (GBM)5 using proteomics, phospho-proteomics, acetylomics, metabolomics and lipidomics data using the GBM dataset from the Clinical Proteomic Tumor Analysis Consortium (CPTAC)6. We developed a computational approach, Substrate PHosphosite-based Inference for Network of KinaseS (SPHINKS) to generate unbiased kinome-phosphosite networks and extract the master kinases (MKs) driving GBM subtypes. We experimentally validated protein kinase Cδ (PKCδ) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) as the MKs that sustain cell growth and tumor cell identity of the glycolytic/plurimetabolic (GPM) and proliferative/progenitor (PPR) functional GBM subtypes, respectively. We confirmed PKCδ and DNA-PKcs as MKs in GPM and PPR tumors from pediatric glioma (PG), breast carcinoma (BRCA) and lung squamous cell carcinoma (LSCC) cohorts classified according to the four functional classes that recapitulate metabolic and proliferation tumor cell states. Finally, we developed a probabilistic classification tool for GBM that exhibits optimal performance in both frozen and formalin-fixed, paraffin-embedded (FFPE) tumor tissue for application in cancer clinical pathology.
Proteogenomic analysis captures functional subtypes of GBM
We recently reported a single-cell-guided, pathway-based classification of isocitrate dehydrogenase (IDH) wild-type GBM that consists of four subtypes within two functional branches: neurodevelopment (PPR and neuronal, or NEU) and metabolism (GPM and mitochondrial, or MTC)5. Here, we used the proteogenomic data of 92 IDH wild-type GBM from the CPTAC cohort that was profiled by genomics, transcriptomics, proteomics, phospho-proteomics, metabolomics, acetylomics and lipidomics to explore the biology associated with the multi-omics taxonomy and uncover therapeutic opportunities (Extended Data Fig. 1a)6. As functional copy-number variations (fCNVs), the CNVs of genes associated with coherent transcriptomic changes in cis and gene expression were the primary data sources for the pathway-based classifier of GBM5, we selected validated fCNVs and transcripts as input features of similarity network fusion (SNF)7 and obtained four stable clusters (Extended Data Fig. 1b). Using 52 GBM classified according to the highest transcriptomic simplicity score as anchors, we classified 33 of the 40 remaining tumors by the SNF distance matrix (Supplementary Table 1a). Genes differentially expressed by each SNF cluster were enriched with biological activities previously assigned to GPM, MTC, PPR and NEU GBM subtypes (Supplementary Table 2a–c)5. Inspection of proteome revealed that the most differentially abundant proteins and enriched pathways coincided with activities biologically congruent with fCNV and gene expression-guided functions and recapitulated the predominant biology assigned to each subtype by SNF clustering (Fig. 1a,b and Supplementary Table 2d,e).
To ask whether fCNVs impact protein abundance in cis, we integrated genomics, transcriptomics and proteomics data to identify genes for which gain or loss correspondingly changed messenger RNA and protein expression (fCNVprot). Overall, 2,205 genes with fCNV gain and 2,837 genes with fCNV loss had concordant changes in protein abundance when compared to copy-number neutral samples (Supplementary Table 2f). Among them, 553 (25.08%) fCNVprot gains and 415 (14.63%) fCNVprot losses segregated with one subtype (Fig. 1c and Supplementary Table 2g–j). fCNVprot contributed directly to activation/deactivation of the subtype-specific biological hallmarks (Extended Data Fig. 1c and Supplementary Table 2k).
To understand the relationship between pathway-based classification (GPM, MTC, PPR and NEU) and previously proposed transcriptional (TCGA: proneural, classical and mesenchymal)8 and epigenetic (MolecularNeuroPathology (MNP): mesenchymal, RTK I, RTK II, RTK III, MID, MYCN and G34)9 subtypes of GBM, we selected 199 and 83 IDH wild-type GBM profiled by both RNA-seq and DNA methylation arrays from TCGA and CPTAC, respectively. We performed a three-way comparison. The GPM subtype exhibited clear association with the mesenchymal subtypes of TCGA and MNP classifiers. Conversely, MTC tumors were mapped to all TCGA and MNP subtypes, with slight preference for RTK II and mesenchymal subtype in the TCGA and CPTAC dataset, respectively (Extended Data Fig. 1d–f and Supplementary Table 1a,b). PPR and NEU had limited overlap with the TCGA and MNP classes, with proneural and RTK I contributing to most PPR and NEU tumors (Extended Data Fig. 1d,e and Supplementary Table 1a,b). Although the epigenetic RTK III, MID, MYCN and G34 subtypes were only minimally represented in TCGA and CPTAC datasets (4.5% and 1.2%, respectively), six of nine tumors were classified as PPR (Extended Data Fig. 1d,e). We also compared functional subtypes with proneural-like, classical-like and mesenchymal-like subtypes reported by CPTAC6. GPM tumors were mainly CPTAC mesenchymal-like; however, the mesenchymal-like group also included a significant fraction of MTC cases (Extended Data Fig. 1f), indicating that our classification uniquely discriminates tumors exhibiting alternative metabolic fluxes (MTC and GPM) and clinical characteristics5. The CPTAC proneural-like subtype included similar fractions of PPR and NEU, whereas the classical-like subtype was preferentially enriched with PPR tumors.
The analysis confirmed orthogonal distribution of MTC GBM and indicated that, with the description of PPR and NEU subtypes, the pathway-based classifier more accurately captures the neurogenesis stages than the vague definition of proneural state.
Proteogenomics enables integrative modules of GBM subtypes
To understand whether each functional subtype of GBM reflects a unique configuration of elements that compose a distinct functional module, from genetic drivers to clinical characteristics such as age, sex and location of the tumor in the brain or radiological features that are obtained at diagnosis by magnetic resonance imaging (MRI), we applied a univariate logistic regression that determined the association of mutations and fCNV5 with each subtype. In an independent model we asked whether proteins encoded by GBM driver genes provide orthogonal validation to the genetic associations (Extended Data Fig. 2). We found that PPR activity predominantly associated with fCNV amplification/mutation/high protein abundance of GBM oncogenes (CDK6, EZH2, MDM4 and EGFR) and fCNV deletion/mutation/protein depletion of CDKN2A, all connected to PPR hallmarks. GPM activity was associated with MET fCNV amplification/high protein abundance and NF1 fCNV deletion/mutation/protein depletion (Extended Data Fig. 2a,c). Confirming our previous findings10, the MTC subtype was associated with FGFR3-TACC3 fusion-positive tumors in the cohort of 178 GBM that we used to validate the probabilistic classifier (see below and Extended Data Fig. 2b)11. fCNV deletion of RERE and SLC45A1 genes located in the ‘metabolic’ region of chromosome 1p36.23 previously identified as a driver of the MTC subtype5 was associated with increased MTC activity. The positive correlation between low RERE protein abundance independently supported the association whereas the SLC45A1 protein was not detected in the CPTAC proteome (Extended Data Fig. 2c). With the limitation of the small number of CPTAC samples, the overall analysis indicated that protein abundance was generally a better indicator of subtype activity than CNV and mutations, a finding that likely reflects control of oncogenic protein abundance by non-genetic factors.
Next, we analyzed the correlation between clinical characteristics and subtype transcriptomic activity. GPM activity showed significant association with male sex and age between 40 and 65 years. When aggregated, PPR and NEU activities approached significance in association with female sex (Fig. 2a). GPM tumors were more frequently found in the frontal and parietal lobes but were excluded from the temporal region. Conversely, MTC tumors were more frequent in the temporal lobe and were excluded from the parietal lobe, indicating a reciprocal brain location pattern for the metabolic subtypes (Fig. 2b).
To interrogate associations between functional GBM subtypes and radiomic features, we used MRI data available from The Cancer Imaging Archive (TCIA)12,13. We categorized the fraction of necrosis, edema and deep white matter invasion and correlated tumor core enhancing and non-enhancing volume and volume of edema with subtype activity (Supplementary Table 1b,c). We also generated an unbiased clustering of histogram-based, volumetric and intensity features. The analyses showed that GPM activity was associated with larger edema and contrast-enhancing volume. PPR activity was associated with greater necrosis, non-enhancing volumes and lower fraction of deep white matter invasion, whereas NEU activity was associated with the lowest volume of necrosis and highest fraction of white matter invasion (Fig. 2c,d). Although the number of samples in each functional subtype was insufficient to provide statistical power, when GPM-MTC or PPR-NEU samples were combined the metabolic subtypes had significantly higher enhancing volume, whereas neurodevelopmental subtypes exhibited larger non-enhancing volumes (Fig. 2e). This scenario was supported by the association of four unsupervised clusters of 175 radiomic features with pathway-based subtypes. Cluster 1 had high non-enhancing and low enhancing volumes as distinctive features and was mostly populated by PPR tumors. Conversely, cluster 4 was enriched with GPM tumors and characterized by overrepresentation of edema and contrast-enhancing volumes but underrepresentation of non-enhancing features (Fig. 2f).
Multi-omics profiling discriminates functional GBM subtypes
We inquired whether the divergent features of GPM and MTC subtypes might independently emerge from proteomics, metabolomics and lipidomics platforms. Comparative analysis of GPM and MTC protein profiles showed significantly higher levels of glycolytic enzymes and lower levels of mitochondrial enzymes (translocases, tricarboxylic acid (TCA) cycle and electron transport chain enzymes) in GPM whereas the reciprocal pattern characterized MTC tumors. GPM GBM was preferentially enriched with intermediates of glycolysis, the pentose phosphate shunt, fatty acids, sugars and essential amino acids, whereas MTC GBM contained higher levels of TCA cycle intermediates, antioxidants and non-essential amino acids (Extended Data Fig. 3a).
The analysis of lipidomic data using LION14 showed that GPM samples had the highest abundance of triacylglycerol, involved in lipid storage and ceramide, which triggers mitochondrial dysfunction (Extended Data Fig. 3b–d and Supplementary Table 2l,m)15–17. Conversely, MTC GBM accumulated acyl-carnitine, an integral component of mitochondrial fatty acid oxidation15 and diacylglycerol, a lipid second messenger required for membrane fusion and fission18. The different lipid composition of GPM and MTC GBM was highlighted by the analysis of lipid cellular components and functions showing enrichment of constituents of lipid droplets in GPM and lipids involved in mitochondrial biogenesis in MTC (Extended Data Fig. 3c,d). Within the neurodevelopmental axis, PPR contained elevated phosphatidylcholines, which are required for cell cycle progression19, whereas NEU tumors were enriched in sphingomyelin, phosphatidylserine, hexosyl-ceramide and cholesteryl ester, all essential components of the myelin sheath that surrounds nerve cell axons20,21 and phosphatidic acid, a central intermediate for the synthesis of neuronal membrane lipids (Extended Data Fig. 3b–d)22.
As lysine acetylation has emerged as a post-translational modification for the regulation of cytoplasmic proteins with crucial metabolic activities and deregulated acetylation of metabolic enzymes can drive metabolic reprogramming of cancer cells23, we inquired whether lysine acetylation might differentially regulate metabolism in GPM and MTC subtypes. Unsupervised clustering of metabolism-related proteins differentially expressed between MTC and GPM tumors revealed two clusters, one enriched with GPM tumors and characterized by accumulation of proteins involved in glucose, amino acid and lipid metabolism, and the other enriched with MTC samples and characterized by accumulation of proteins associated with mitochondrial metabolism (Extended Data Fig. 3e and Supplementary Table 3a,b). By applying the outlier enrichment analysis (BlackSheep)24 to acetylated proteins, we found that in contrast to global protein abundance, the highest acetylated metabolic proteins in GPM samples included mitochondrial enzymes, whereas MTC samples exhibited hyperacetylation of enzymes implicated in glycolysis and the pentose phosphate pathway as well as amino acid biosynthesis and adipogenesis (Extended Data Fig. 3f and Supplementary Table 3c). As acetylation has been viewed as an inhibitory post-translational modification for the activity of metabolic enzymes25, these results present additional levels of coordination of the alternative reprogramming in the metabolic axis of GBM subtypes.
We then examined the pattern of nuclear protein acetylation across GBM subtypes. Unsupervised clustering of the most variable nuclear protein acetylation sites uncovered three clusters (Fig. 3a). Cluster 1 was acetylation cold and enriched in GPM and NEU tumors. Cluster 2 included tumors with the highest acetylation and was almost exclusively composed of PPR samples. Cluster 3 was an intermediate/low-acetylation cluster that included 46% of PPR samples (16 tumors) intermixed with GPM, NEU and MTC tumors (Fig. 3b). Thus, the PPR subtype seems to be divided into two subgroups, exhibiting high and low nuclear protein acetylation, respectively (Fig. 3c and Supplementary Table 3d). Tumors in the high-acetylation PPR subcluster had the highest proteomics but not transcriptomics proliferation/stemness scores, thus highlighting the specific role of the post-translation acetyl modification in this subtype (Fig. 3d,e). Differential acetylation of PPR GBM among high-acetylation and low-acetylation subclusters involved specific acetylation sites of histone and non-histone acetyltransferases (lysine acetyltransferases, KATs) whose enzymatic activity is activated by auto-acetylation26,27. Such activation was clearly manifested in high-acetylation PPR by the elevated level of acetyl-lysines in the HAT domain of p300 (K1554, K1555, K1558 and K1560) and functionally similar residues in the HAT domain of other KATs such as members of the MYST complexes (MEAF6, ING4, JADE2, JADE3 and MYST3; Fig. 3f and Supplementary Table 3e). The latter introduce acetylated marks upon histones H2, H3 and H4 (ref. 28), which were recovered as hyperacetylated (H2AX, H2AFV and HIST2H4B) in high-acetylation PPR. Besides KATs and histones, chromatin-modifying enzymes and enzymes involved in DNA damage response (DDR) and DNA replication stress (RS) were hyperacetylated in high-acetylation PPR, suggesting that acetylation contributes to the activation of these biological functions in PPR GBM (Fig. 3g).
Sustained RS and DDR signaling characterizes PPR GBM
The proteomic profiling of PPR GBM combined molecular marks of proliferation with activation of DDR (Fig. 1b). Moreover, PPR tumors exhibited overrepresentation of DNA replication/replication fork and DNA double-strand break repair (DDSB) proteins, suggesting that enhanced RS may promote DDR signaling (Fig. 4a). To test this hypothesis, we performed data mining and ontology integration from mass-spectrometry datasets to identify phosphosites increased in cells treated with irradiation, which causes DDSB lesions, ATR inhibitors or hydroxyurea that induce RS (Methods). We selected 15 and 16 experimentally validated phosphosites specific for cells undergoing DDSB and RS, respectively and 3 phosphosites common to DDSB and RS. Compared to other tumor subtypes, PPR contained elevated levels of 11 (73.3%) and 10 (62.5%) of DDR and RS signature phosphosites, respectively (Fig. 4b and Supplementary Table 4). Using DDR and RS phospho-proteomic signatures, we computed DDR and RS enrichment scores for each tumor and found higher scores in PPR than other subtypes, with the NEU group characterized by the lowest scores (Fig. 4c, top). The highest PPR scores were retained even when tumors were classified according to the difference between proteomic and transcriptomic subtype activity (Fig. 4c, bottom), thus reinforcing the significance of the proteome for the association between DDR/RS and PPR subtype. Western blot using CHK1-ser-317 phosphorylation as a basal DDR biomarker of ATR-activated CHK1 (ref. 29) showed that GBM patient-derived organoids (PDOs) classified as PPR5 exhibited higher levels of basal DDR/RS than GPM PDOs (Fig. 4d).
Master kinase analysis uncovers GBM subtype-specific kinases and actionable dependencies
To begin exploring the phospho-proteomics landscape of GBM subtypes and their organization, we cataloged phosphosites specific for each GBM subtype and applied the outlier enrichment analysis. We obtained four phosphosite modules of overrepresented pathways that summarized previously assigned subtype hallmarks (Fig. 5a and Supplementary Table 5a–c). We then sought to link phosphosite enrichment to the activity of GBM subtype-specific protein kinases. To this aim, we developed SPHINKS, which integrates proteomics and phospho-proteomics profiles to build an interactome of kinase–phospho-substrate pairs that are scored according to the strength of their interaction across all samples (Fig. 5b). The GBM-specific kinase–phosphosite interaction network was generated using a semi-supervised support vector machine (SVM) algorithm trained on experimentally validated kinase–substrate phosphosite pairs from the PhosphoSitePlus database30. SPHINKS produced a GBM kinase–phosphosite interactome comprising 13,866 predicted interactions between 154 kinases and 3,186 phospho-substrates (Extended Data Fig. 4a(i–iv)). To benchmark SPHINKS, we assessed the impact of missing data in the kinase–phosphosite interactome by comparing networks reconstructed from the CPTAC-GBM un-imputed matrix of phosphosites lacking missing values (gold standard, 7,302 phosphosites) and controlled simulations of imputed matrices composed of different ratios of phosphosite missing values (Methods). Receiver operating characteristics (ROC) analysis showed that regardless of the different thresholds of missing values, the area under the curve (AUC) was consistently close to 1, indicating that the output of SPHINKS was not affected by missing values (Extended Data Fig. 4b). To evaluate the accuracy of SPHINKS to correctly predict kinase–phospho-substrates, we performed a tenfold cross-validation by randomly dividing validated interactions into ten subsets for training and testing. AUC values of all iterations between 0.86–0.89 indicated high prediction accuracy (Extended Data Fig. 4c). As some of the selected phosphosites in the negative test set might be true substrates, AUC values are likely to be underestimated. To test the stability of SPHINKS kinase activity estimates, we generated 100 independent networks for each kinase and perturbed them by replacing a predetermined percentage of phospho-substrates with random phosphosites. Average Δ activity scores (difference between unperturbed and perturbed networks) indicated a remarkable stability of the kinase activity estimate inferred by SPHINKS (median Δ activity = 3%, for perturbations ≤20% interactions in both analyses; median Δ activity = 4% in both analyses, maximum of 10% in kinase analysis, for perturbations of 50%; Extended Data Fig. 4d).
To uncover MKs associated with distinct GBM subtypes, we implemented single-sample MK analysis by computing the weighted strengths of connectivity between kinase and predicted substrate phosphosites against random phosphosites for each tumor and testing the contribution of each MK in each subtype by Mann–Whitney–Wilcoxon (MWW) test10 (Extended Data Fig. 4a(v) and Supplementary Table 5d). GPM, PPR and NEU GBM exhibited rich and interconnected kinase–substrate networks as opposed to the MTC subtype that was sustained by a more limited network (Fig. 5c and Extended Data Fig. 4e). Mapping the predicted subtype-specific MKs onto the human kinome tree showed a random distribution across kinase families (Extended Data Fig. 4f). We validated subtype-specific MKs in bulk GBM, single-cell RNA-seq (scRNA-seq) data from 17,367 GBM cells and 79 GBM PDOs5. mRNA and protein of the kinases identified by SPHINKS-MK were generally upregulated in bulk tumors and cells of the corresponding subtype (Fig. 5d and Supplementary Table 5e). We compared SPHINKS-MK with kinase–substrate enrichment analysis (KSEA)31 and kinase enrichment analysis 3 (KEA3)32. Unlike SPHINKS that reconstructs context-specific kinase–phospho-substrate networks and detects potentially new kinase–substrate interactions, KSEA and KEA3 derive kinase activity from networks of experimentally validated phospho-substrates. For KSEA, we obtained kinase activities from validated interactions from PhosphoSitePlus (KSEA PhosphoSitePlus) or predicted relationship from NetworKIN (KSEA PhosphoSitePlus + NetworKIN). For KEA3, we applied MeanRank and TopRank for ranking the integrated kinase activity from 11 protein–protein and kinase–substrate interaction libraries. We used a dataset reporting changes in the abundance of phospho-proteins after perturbation of upstream kinases33,34 (103 kinase perturbation for 30 kinases and 61,181 phosphosites, the ‘gold standard’) and the metric defined as ‘top-k-hit’, which focuses on the top kinase predictions34. SPHINKS produced higher activity scores than other methods and was superior in correctly identifying the perturbed kinases (Extended Data Fig. 5a). We also calculated the difference between the activity rank inferred by SPHINKS and each of the other methods (Δ rank score) of 129 kinases common to all five methods for each GBM subtype using CPTAC-GBM proteomic/phospho-proteomic data. For all comparisons, most of the kinases exhibited a negative Δ rank score, indicating that SPHINKS has a consistently higher predictive power than other approaches (Extended Data Fig. 5b).
PKCδ and DNA-PKcs are subtype-specific actionable MKs in GPM and PPR
The application of SPHINKS-MK uncovered PKCδ as the top-scoring MK of the GPM subtype (Fig. 5c). PKCδ controls crucial steps of glucose and lipid metabolism in multiple tissues35. In cancer, PKCδ is a central signaling node of the insulin–IGF–AKT–mTOR pathway that orchestrates metabolic reprogramming toward aerobic glycolysis and increased uptake of nutrients36–38. PKCδ also mediates resistance to antitumor therapies possibly by upregulating glucose uptake in cancer cells39. As the metabolic functions controlled by PKCδ are hallmarks of GPM GBM5, we tested the role of PKCδ in the plurimetabolic phenotype and viability of this subtype. Exposure of GBM PDOs classified as GPM to eight compounds targeting different glycolytic enzymes or irradiation confirmed that each treatment was ineffective in these cells (Fig. 6a). Next, we asked whether activation of PKCδ in GPM GBM segregated with insulin-IGF-AKT signaling. By the comparative analysis of protein and phospho-protein abundance of pathway-specific signaling molecules in GPM versus all other subtypes, we found that crucial components of the insulin–IGF–AKT pathway were activated in GPM tumors by elevation of protein abundance and/or phosphorylation, and co-segregated with PKCδ abundance and activation (Extended Data Fig. 6a). AKT1/2 and STAT3, central nodes in insulin–IGF-PKCδ signaling, were activated in GPM GBM. Additionally, activation of the mTOR kinase (RAPTOR-ser-863) and substrates (p70S6K and 4E-BP-ser-37/thr-46 phosphorylation) was consistent with the relevance of this pathway for the metabolic reprogramming of GPM tumors (Extended Data Fig. 6a). Stimulation of GPM PDOs by IGF1/2 and insulin induced phosphorylation of PKCδ on tyr-311, a phosphosite crucial for its activity40, concurrently with AKT-thr-308 and ser-473 phosphorylation (Extended Data Fig. 6b,c). To test the essentiality of PKCδ for fitness and the plurimetabolic state of GPM cells, we treated GPM PDOs with BJE6-106 (ref. 41), a third-generation inhibitor of PKCδ and found that most of the tested models exhibited marked sensitivity to PKCδ inhibition (Fig. 6b). BJE6-106 also caused dose-dependent inhibition of colony formation (Fig. 6c) and time-dependent decrease of AKT-ser-473 and STAT3-tyr-705 phosphorylation (Fig. 6d). Genetic knockdown of the PRKCD gene (Fig. 6e) corroborated the requirement of PKCδ for growth and viability of GPM PDOs (Fig. 6f–h) as well as glucose uptake and lipid accumulation (Fig. 6i,j).
The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) was among the most active MK in the PPR subtype of GBM (Fig. 5c,d). DNA-PKcs is one of the three members of PIKKs with principal roles in the activation of DDR. DNA-PKcs is activated by multiple types of genotoxic stress, including DDSB and RS42,43. Given the specific activation of DDR and RS in PPR GBM (Figs. 1b, 3g and 4), we postulated that active DNA-PKcs may counter the increased rates of DNA replication and DDR in PPR cells. Consequently, we asked whether inhibition of DNA-PKcs with M3814 (nedisertib), a DNA-PKcs inhibitor currently in clinical studies44, promotes vulnerability of PPR GBM when used in combination with ionizing radiation (IR), the key element in the standard of care for patients with GBM. Treatment of PPR GBM PDOs with a nedisertib–IR combination markedly reduced tumor cell viability compared to each individual treatment, with a radiation dose enhancement factor (DEF) > 2 for six PPR PDOs. Conversely, nedisertib–IR combination was ineffective in GPM PDOs (Fig. 6k and Extended Data Fig. 6d). We confirmed these results using the clonogenic assay as a quantitative method of radiosensitivity (Extended Data Fig. 6e). Exposure of PPR PDOs to IR rapidly induced phosphorylation of DNA-PKcs ser-2056, the key autophosphorylation site marking kinase activation45. As expected, nedisertib inhibited ser-2056 phosphorylation in irradiated cells (Fig. 6l). Combinatorial treatment caused persistent DNA damage as shown by sustained phosphorylation of ser-343 of NBS1 and ser-824 of KAP1, indicators of active DDSB, as opposed to rapid de-phosphorylation in PDOs exposed to IR alone (Fig. 6l). Consistently, the number of γ-H2AX foci, which regressed to basal levels in PPR cells treated with irradiation alone, remained elevated throughout the course of the experiment in the presence of DNA-PKcs inhibition (Fig. 6m).
Functionally conserved pediatric and adult cancer subtypes share MKs
In an effort to ascertain whether the key biological functions discriminating the GBM subtypes coalesce into grouping patterns sharing the same kinase-driven dependencies, we first determined whether a functional classification could be obtained in PG, BRCA and LSCC for which genomics, proteomics and phospho-proteomics datasets are available46–48.
For PG, we integrated protein and gene expression data of 103 samples classified as high-grade (PG-HGG) or low-grade (PG-LGG) gliomas using SNF (Supplementary Tables 1d and 6a). We identified four subtypes of PG, recapitulating the functional classifier of GBM for proteomic, phospho-proteomic and gene expression data (GPM, MTC, PPR and NEU; Fig. 7a and Supplementary Table 6b–g). PG-HGG mostly clustered within the PPR subtype, whereas PG-LGG was distributed across the four subgroups (Fig. 7a,b). When PG-HGG and PG-LGG were analyzed independently for differential protein abundance, high- and low-grade tumors clustered into three and four groups, respectively, with the MTC subtype excluded from PG-HGG (Extended Data Fig. 7a,b and Supplementary Table 6h–k). BRAF KIAA1549-BRAF fusions and BRAF-V600E mutation are common in PG-LGG49. Glioma harboring BRAF-V600E were mostly classified as MTC, whereas PG-LGG harboring KIAA1549-BRAF fusion or BRAF wild-type were enriched with GPM and NEU tumors, respectively (Fig. 7a,c). Kaplan–Meier and log-rank test demonstrated significantly worse survival for the PPR subtype, a finding compatible with the predominant contribution of high-grade tumors to this group (Extended Data Fig. 7c).
We also classified 118 BRCA samples into four subtypes having coherent gene expression, protein and phospho-protein abundance signatures. The three major groups represented 95% of the samples (GPM, PPR and MTC), whereas the NEU group included only five tumors (Fig. 7d and Supplementary Tables 1e and 7a–g). We found a striking association of the HER2-I (I, inclusive as defined by integrative CPTAC analysis) subgroup with the GPM subtype, Basal-I with PPR, LumA-I with MTC and LumB-I with NEU (Fig. 7e). Enrichment of HER2-I in the GPM subtype is consistent with hyperactivation of mTOR and a metabolic shift from aerobic respiration to glycolysis in this BRCA subtype50. The stability of the functional classification of BRCA was verified using TCGA and METABRIC gene expression data, thus authenticating the biological activities as general features for BRCA categorization (Extended Data Fig. 8a,b and Supplementary Tables 1f,g and 7h–k). The positive association between PPR and Basal-I subtype was further supported by the strong enrichment of DNA replication and proliferation-associated pathways in the Basal-I subtype (Fig. 7d). Consistent with the prolonged survival of LumA-I, the MTC-BRCA subtype had a significantly better prognosis (Extended Data Fig. 8c).
Finally, we used the functional classifier to segregate 106 LSCC tumors and tested the association with the five known LSCC-specific molecular NMF-based subtypes described by CPTAC (Fig. 7f,g and Supplementary Tables 1h and 7l–r). LSCC tumors were classified into two major subtypes (GPM and PPR) and a much smaller MTC subgroup. In this limited dataset we did not identify NEU tumors. We found a positive correlation of the MTC subtype with the Basal-I subgroup. EMT and inflamed secretory LSCC subtypes as two independent groups were functionally unified by the activation of immune, epithelial-to-mesenchymal transition (EMT) and angiogenesis functions of the GPM subtype. The PPR subtype included proliferative-primitive and classical subtypes, both sustained by proliferative-related pathways (Fig. 7f,g)48,51. The robustness of the functional subtyping was validated in the TCGA-LUSC (lung squamous carcinoma) dataset (Extended Data Fig. 8d and Supplementary Tables 1i and 7s,t). In this larger cohort, 12 tumors exhibited activation of synaptic functions, a hallmark of the NEU subtype. MTC-LUSC tumors exhibited more favorable clinical outcomes, suggesting that also in this tumor type OXPHOS activation produces a less aggressive biology and/or increases sensitivity to therapy (Extended Data Fig. 8e)5. Dependency of BRCA and LUSC MTC cells on mitochondrial activity was supported by the association between MTC activity of BRCA and LUSC cell lines in the DepMap dataset52 and sensitivity to menadione, a cytotoxin that specifically targets mitochondria (Extended Data Fig. 8f).
Next, we applied SPHINKS to generate tumor-specific kinase–phosphosite interactomes for PG, BRCA and LSCC, including 669, 1,399 and 1,985 kinase–phosphosite relationships from 76, 198 and 103 kinases and 210, 1,899 and 699 phosphosites for PG, BRCA and LSCC, respectively and identified subtype-specific MKs (Supplementary Tables 8–10 and Extended Data Fig. 9) that we validated by global protein abundance and mRNA expression (Supplementary Tables 8–10). Most subtype-specific MKs were activated only in one tumor type (Extended Data Fig. 9). Among top-ranking tumor-specific MKs, FYN was MK of the GPM subtype in BRCA. FYN is a member of the SRC family of kinases driver of EMT in breast cancer53,54. VRK1 was among the top-ranking PPR MKs in BRCA. VRK1 is a chromatin-associated kinase that regulates cell cycle events and DDR previously proposed as therapeutic target in combination with DNA damage inducing therapy55,56. Nine protein kinases emerged as top-ranking subtype-specific MKs common to GBM, PG, BRCA and LSCC. Among them, PKCδ scored as pan-GPM and DNA-PKcs as pan-PPR MKs (Fig. 7h).
Development of a probabilistic functional classifier of GBM
We designed an algorithm for the probabilistic classification of individual tumors into GBM functional subtypes. When compared to RNA derived from fresh frozen samples, FFPE-extracted RNA is characterized by lower quality, typically affecting different mRNA species to variable extent57. Thus, we tested two classifiers, one informed by RNA-seq data from frozen tumor samples (‘frozen model’) and the other by RNA-seq data from FFPE tumors (‘FFPE model’). For the frozen model, we trained the classifier using the multinomial regression model with lasso penalty on the TCGA IDH wild-type GBM dataset profiled by Agilent expression array, which we had classified in previous work (Extended Data Fig. 10a and Supplementary Table 11a)5. As a feature set, we selected the 50 highest ranking genes for each functional subtype (a total of 200 gene features)5. To extract a reduced number of features that maximize the distinctiveness of the phenotypes, we applied a cross-validation approach and selected the model exhibiting the lowest misclassification error (17.19% cross-validation error and 6.32% error on the training set), obtaining 103 gene features with positive or negative coefficients (Supplementary Table 11b). We classified a tumor sample when the fitted probability was the highest and the simplicity score was above a predefined threshold (Methods). We tested the prediction ability of the ‘frozen classifier’ using 127 GBM from TCGA and 85 GBM from CPTAC profiled by RNA-seq. We classified 80% and 79% of the TCGA and CPTAC-GBM, respectively. The diagnostic ability of the classifier was confirmed by the AUROC of each subtype above 0.85 in each validation dataset (Fig. 8a). We determined the accuracy of the assignment of each tumor to the correct subtype58. Misclassification error was < 18%, sensitivity approached 85%, specificity was close to 100% and precision > 80%, indicating a robust performance of the classifier (Fig. 8b and Supplementary Table 11c). The frozen model was validated on an independent cohort of 45 frozen samples for which matched FFPE samples were available (see below), obtaining similar results (Extended Data Fig. 10b).
For the FFPE model, to account for the lower quality of FFPE-extracted RNA, we sequenced the transcriptome of 45 frozen and FFPE matched samples and selected 4,668 genes that exhibited consistent expression profiles in both sample types (genes supposedly unaffected by FFPE treatment, Spearman correlation, ρ > 22; Supplementary Table 12). With the classification of frozen samples as the gold standard, we generated subtype-specific signatures using expression profiles of the corresponding FFPE samples. We then trained the multinomial regression model using FFPE-specific signature genes from TCGA-GBM Agilent cohort (66 gene features, 19.76% cross-validation error and 11.07% error on the training set). The performance of the classifier was assessed on an independent cohort of 133 FFPE samples profiled by RNA-seq, classifying 73% of the samples. To assess the stability and accuracy of the FFPE model, we unbiasedly assigned FFPE samples to a subtype by unsupervised consensus clustering of 178 samples (133 FFPE plus 45 FFPE with matched frozen specimens; Extended Data Fig. 10c). Using the classification of the 45 frozen samples as ‘anchors’, we assigned each cluster to a functional GBM subtype and compared the resulting unbiased label assignment with the subtype classification from the FFPE model for the 133 unmatched FFPE samples only. The classifier performance indexes were similar to those calculated for the frozen model (misclassification error of 15%; AUROCs, sensitivity, specificity and precision > 0.84; Fig. 8c,d and Supplementary Table 11b,c). The FFPE model was also validated on 45 FFPE samples using the classification of the matched frozen specimens as ground truth, obtaining comparable results (Extended Data Fig. 10d).
We have implemented a Shiny app of the frozen and FFPE classification tools for general research use at https://lucgar88.shinyapps.io/GBMclassifier.
Discussion
Here, we sought to establish a link between multi-omic features that regulate the biology of GBM subtypes and protein kinases that could directly enable subtype-specific phenotypes. We built and applied SPHINKS-MK, an algorithm that integrates proteomics and phospho-proteomics datasets into a single network for the unbiased extraction of subtype-specific MKs. By informing pharmacologic and genetic experiments in subtype-matched GBM organoids, SPHINKS-MK delivered PKCδ and DNA-PKcs as experimentally validated MKs for the aggressive GPM and PPR subtypes of GBM. The four subtypes and the underlying phenotypes were also recovered across different tumor types, highlighting the fundamental biological traits that are extracted by the functional classification. In the multi-cancer context, PKCδ and DNA-PKcs have emerged as broadly actionable MKs of GPM and PPR subtypes. Inspired by the subtype-specific therapeutic opportunities, we present a probabilistic classifier that enables rapid translation of precision therapeutics for subgroups of patients with GBM.
The four GBM subtypes initially inferred from a pathway-based scRNA-seq analysis are supported by orthogonal analyses from proteomics, phospho-proteomics, metabolomics, lipidomics and acetylomics platforms. The divergent metabolism of the GPM and MTC subtypes was independently captured by the analysis of acetylomics, a post-translational modification previously associated with the inactivation of metabolic proteins25. Acetylation also emerged as major determinant factor instructing the identity of the proliferation-, stemness- and DDR-related biology that is activated in PPR cells. Stratification of PPR GBM based on acetylation of nuclear proteins uncovered a hyperacetylated PPR group of tumors with outlier activation of these activities. This finding underscores the crucial role of acetylation of nuclear proteins for activation of transcription and chromatin-remodeling factors and enzymes involved in the DDR59. The significance of the pathway-based classification of GBM is further emphasized by the association of the individual subtypes with clinical variables such as age and tumor location within the central nervous system and frequency of recurrent alterations of driver genes. The interrogation of MRI features associated with each subtype showed that the metabolic subtypes, and particularly the GPM subgroup, are characterized by higher contrast enhancement, potentially reflecting more prominent perivascular invasion of tumor cells with consequent disruption of the endothelial tight junctions of the blood–brain barrier. Conversely, tumors classified along the neurodevelopmental axis are associated with non-enhancing features. Among them, the unique correlation between NEU tumors and deep white matter invasion is consistent with the proposed ability of neuronally differentiated GBM cells to engage healthy brain cells at the tumor periphery for neomorphic synaptic connections that guide invasion through white matter tracks5 (Fig. 8e).
Although prediction of active protein kinases in cancer has been so far of limited impact for cancer therapy, there is tremendous appeal of kinases as both drivers and drug targets. SPHINKS-MK interrogated the full scope of tumor-specific kinomes and phosphorylomes reconstructed into an integrated functional network and identifies high-activity kinases specific for tumor subtypes. The benchmarking of SPHINKS showed that the algorithm is stable and exhibits a prediction power higher than other inference methods. PKCδ emerged as the top-scoring kinase of the GPM subtype. Genetic and pharmacologic inhibition of PKCδ defined its role in oncometabolic processes at the intersection of insulin, IGF and lipid metabolism and validated PKCδ as crucial therapeutic target of the GPM subtype of GBM. DNA-PKcs was experimentally validated as essential MK of the PPR subtype. The synergistic and lethal effect of inhibition of DNA-PKcs and IR in PPR but not GPM cells provided the mechanistic interpretation of therapy resistance in this GBM subtype. As DNA-PKcs inhibitors have been introduced into clinical trials44,60, our findings indicate that preselection of patients with PPR tumors is likely to enhance therapeutic success. The GBM classifier was validated as a stratifying method for pediatric and adult tumors, revealing consistent patterns across different tumor types (for example, favorable survival associated with MTC tumors) and context-dependent features (BRAF mutations and fusions associated with divergent metabolic subtypes in PG). The identification of PKCδ and DNA-PKcs as subtype-specific MKs from SPHINKS-inferred PG, BRCA and LSCC kinase–phosphosite interactomes delivers targeted therapeutic directions for GPM and PPR subtypes across multiple tumor types.
The probabilistic classification tool will facilitate the yet unfulfilled stratification of patients with GBM for the accrual to clinical trials using FFPE specimens and advance precision therapies targeting individual subtypes of this aggressive tumor.
Methods
Ethics statement
PDOs have been described previously5. PDOs were obtained using excess material collected for clinical purposes from specimens de-identified at the source. This work was designated Institutional Review Board exempt under paragraph 4 and covered under Institutional Review Board and Onconeurotek tumor bank certification (NF S96 900) and authorization from an ethics committee (CPP Ile de France VI, ref. A39II) and the French Ministry for Research (AC 2013–1962).
Patient datasets and profiling platforms
For each cancer type6,9,46–48,61–63, multi-omics data availability, tumors analyzed, clinical and survival data are listed in Supplementary Table 1.
Data processing
Gene expression
Data from CPTAC were downloaded as fpkm. Non-protein-coding and low-expressed genes were removed. Data were quantile and log2 normalized. Data from METABRIC (Illumina HT-12 v.3) were downloaded as median normalized. RNA-seq data from TCGA were downloaded using TCGAbiolinks. Upper quantile within-normalization with GC content correction and between-normalization were applied.
DNA methylation
Data from CPTAC (EPIC array) were downloaded as β-values, pre-processed with functional normalization with minfi64, quality checked, with common single-nucleotide polymorphism filtering and probe annotation. Values missing in < 20% across all sample were imputed using the average of the corresponding probe. Data from TCGA were pre-processed with functional normalization and probes targeting sex chromosomes or not associated with gene promoters65 were removed. Processed β-values and classification of the MNP cohort were downloaded from the Gene Expression Omnibus (GSE90496, MNP reference set) and supplementary tables published previously9.
Copy number
Thresholded CNVs were assessed using GISTIC. Protein-coding genes were retained. fCNVs were obtained as described5.
Global proteome and phospho-proteome
Values missing in <50% across all samples were imputed with DreamAI66 and were quantile and log2 normalized.
Lipidome and metabolome
Data were downloaded as log2-tranformed and median normalized. Values missing in fewer than five or ten tumors for lipids or metabolites, respectively, were imputed using average abundance of the corresponding molecule. Data were quantile normalized.
Acetylome
Data were imputed with DreamAI and log2-transformed.
Functional classification of CPTAC IDH wild-type GBM
We used Agilent expression profiles of 304 TCGA-GBM IDH wild-type previously classified5 as training set of a k-nearest neighbors (k-NN) classifier (k = 3) to classify CPTAC tumors. To account for differences in gene expression between TCGA and CPTAC, we generated ranked lists of genes differentially expressed in each CPTAC subtype compared to the others using the MWW test and defined as subtype-specific signatures the 50 highest scoring genes. For each tumor, we derived the intensity of each subtype as the average expression of genes in each subtype-specific signature. A simplicity score was obtained as the difference between the two highest subtypes intensities, and tumors with simplicity score > 0.6 were retained (17 GPM, 6 MTC, 16 NEU and 13 PPR core samples).
To assign membership to 40 unclassified tumors, we integrated fCNV and gene expression using SNF for 89 tumors. The features set of the classifier (subtype-specific fCNV gains/losses from TCGA and subtype-specific gene signatures from CPTAC core samples) were aggregated by SNFtool to generate a fused tumor network and a tumor similarity matrix (K = 20, α = 0.5 and t = 20). Spectral clustering was performed on the similarity matrix. The distance matrix (1 − similarity) was used to establish membership of 38 unclassified GBM according to the closeness to core tumors with k-NN (k = 3). Five tumors with conditional probability < 0.6 remained unclassified.
Cross-classification analysis
We classified TCGA- and CPTAC-GBM samples according to MNP DNA methylation classification9 using MNP-GBM and assignment as training set of k-NN. The top 10,000 variable probes shared by MNP and TCGA or CPTAC samples were selected. We extracted the top 30 principal components by principal-component analysis and assigned an MNP classification to TCGA or CPTAC samples using k-NN (k = 9)6. While an official MNP classifier exists online (https://www.molecularneuropathology.org/mnp), we were not able to access it as the site did not approve our registration at the time of writing.
To assess the relationship between pathway-based classification and transcriptional subtyping in TCGA- and CPTAC-GBM, we analyzed 304 TCGA-GBM previously classified5. TCGA subtype assignments were obtained as described8. Subtyping of CPTAC tumors was described previously6.
Multi-omics characterization of GBM functional subtypes
We generated ranked lists of genes, proteins, lipids and metabolites differentially expressed/abundant in each subtype compared to the others by MWW test. Final subtype-specific signatures including the 150 top-scoring genes or proteins were used to calculate subtype enrichment in each tumor using single-sample MWW-GST (ssMWW-GST). Pathway enrichment analysis was performed as described elsewhere5, using non-redundant pathways from a set cover algorithm67. The most active pathways in each subtype were obtained using gene or protein ranked lists by two-sided MWW-GST (logit(NES) > 0.58, FDR < 0.005).
Enrichment of glycolytic and mitochondrial enzymes (protein sets) and metabolic intermediates (metabolite sets) in MTC and GPM were generated by MWW-GST (glycolytic enzymes: logit(NES) = 1.27, P = 0.017; mitochondrial enzymes: logit(NES) = −1.19, P = 5.93 × 10−13; glycolytic intermediates: logit(NES) = 1.76, P = 0.0007; mitochondrial intermediates: logit(NES) = −1.65, P = 0.018). The network of metabolites and metabolic proteins was constructed using Ingenuity Pathway Analysis (IPA)68.
Lipid signatures included molecules with an MWW score > 0.5. Lipids were categorized and used for enrichment of lipid subclasses, cellular components and lipid functions in each subtype using Fisher’s exact test (FET; log(OR) > 0, P < 0.05) and the lipid ontology database LION14.
Proteogenomic integrative analysis of GBM
fCNVprot were obtained by integrating fCNVs, gene expression, and protein abundance of genes that exhibited fCNV change in two or more tumors according to the following criteria: (1) higher/lower protein abundance in tumors with alteration compared to wild-type (|log2 (FC)| > 0.15, P < 0.10; two-sided MWW test); (2) higher/lower protein abundance in one subtype compared to the others (|log2 (FC)| > 0.15, P < 0.10; two-sided MWW test); (3) higher subtype-specific transcriptomic activity of tumors harboring the fCNV compared to wild-type (effect size > 0.15, P < 0.10; two-sided MWW test). Subtype-associated fCNVprot gains/losses were examined for their contribution to activation/deactivation of biological pathways using FET (P < 0.05).
Univariate logistic regression analysis
Tumors were segregated according to fCNV status (altered, wild-type); subtype activity was a continuous predictor. Additionally, tumors were segregated according to subtypes and protein abundance was used as a continuous predictor. The analysis of FGFR3-TACC3 fusion included 178 GBM FFPE RNA-seq samples (fusion present, 12 tumors or absent).
Analysis of acetylation of metabolic and nuclear proteins
We used 2,212 genes from the Reactome Metabolism gene set to define proteins involved in metabolism. Unsupervised clustering was performed on proteins differentially expressed between GPM and MTC (P < 0.05, log2(FC) > 0.3; two-sided MWW test).
Normalized acetyl site abundance (acetylation not explained by the corresponding protein abundance) was calculated as residuals (εsite) from the linear regression Acsite = β0 + β1 × Prsite + εsite, where Acsite is the abundance of a given acetyl site and Prsite is the corresponding protein abundance.
We applied BlackSheep’s differential extreme value analysis module to define outlier acetylated metabolic proteins (P < 0.05) and enrichment of biological pathways using FET (P < 0.0005).
Nuclear proteins were selected by the COMPARTMENTS database69 (nucleus score of 5). Acetyl sites with the highest variability across the dataset by interquartile range (n = 320) were used for unsupervised clustering. Differentially abundant acetyl sites in high- versus low-acetylation PPR subgroups were defined by MWW test (P < 0.001, log2(FC) > 0.3). Acetyl sites whose abundance was not explained by protein abundance were selected by comparing global protein and acetyl site abundance between high- and low-acetylation PPR subgroups using MWW test (log2(FC) > 0.5, P < 0.05). Pathway overrepresentation testing was performed using gProfiler tool (FDR < 0.05).
Generation of replication stress/DNA damage response phospho-proteomic signature
We manually curated data from five studies reporting mass spectrometry phospho-proteomics70–74 to identify sites whose phosphorylation was increased after induction of DNA RS by ATR inhibition or hydroxyurea treatment or DDR by IR exposure. Differential abundance of DDR/RS-induced-phosphosites was performed comparing PPR subgroup versus the others (P < 0.05; MWW test). DDR/RS phospho-signatures were used to calculate DDR/RS scores in each tumor (ssMWW-GST). Enrichment of GPM, MTC, NEU and PPR tumors in highest/lowest distribution of the DDR/RS score (|logit(NES)| > 0) was tested using FET. Difference between transcriptome- and global proteome-derived subtype activity was calculated and the association with DDR/RS score tested using Spearman’s correlation.
Functional classification, analysis and validation of PG, BRCA and LSCC
We used RNA-seq expression profiles of 105 CPTAC-PG, 119 CPTAC-BRCA and 108 CPTAC-LSCC to compute the enrichment of the functional subtype-specific signatures from TCGA-GBM in each tumor and protein abundance data to compute the enrichment of the 50 highest scoring proteins in the ranked list of each CPTAC-GBM subtype in each tumor using ssMWW-GST. Tumors were classified according to the subtype with the concordant highest NES (logit(NES) > 0.3, FDR < 0.05) in both transcriptomic and proteomic data and were defined as ‘anchor tumors’ (51, 54, 64 tumors for PG, BRCA and LSCC, respectively). We used anchor tumors to generate ranked lists of genes and proteins (MWW test). Tumor type-specific/subtype-specific gene and protein signatures included the top 50 scoring genes and proteins. Unclassified tumors (54 PG, 96 BRCA and 44 LSCC) were classified by integrating gene and protein signatures from the previous step using SNF. Final classifications include 48 GPM, 7 MTC, 27 NEU, 22 PPR and 1 unclassified for PG; 50 GPM, 23 MTC, 5 NEU and 40 PPR for BRCA; and 51 GPM, 9 MTC, 0 NEU, 46 PPR and 2 unclassified for LSCC samples. We used the expression profiles of 1,095 tumors from TCGA-BRCA, 1,904 tumors from METABRIC-BRCA and 502 tumors from TCGA-LUSC to compute the enrichment of functional subtype-specific signatures from TCGA-GBM in each tumor (ssMWW-GST), classifying them according to the subtype with the highest NES (logit(NES) > 0.58, FDR < 0.05).
Normalized phosphosite abundance (phosphorylation not explained by the corresponding protein abundance) was calculated as for normalized acetyl site abundance, using the abundance of the phosphosite and corresponding protein.
Association between functional classification and tumor grade, BRAF status (PG) or CPTAC NMF-derived subtypes (BRCA and LSCC) was assessed by chi-squared test. Survival analysis among functional subtypes in TCGA-BRCA, TCGA-LUSC and METABRIC-BRCA was assessed by log-rank test.
DepMap data analysis
Transcriptomic profiles of BRCA and LUSC cell lines from DepMap for which both RNA-seq expression and menadione survival ratio from PRISM Repurposing Primary Screen were available (BRCA, n = 26; LUSC, n = 71)52 were used to derive subtype activities and classification according to the highest NES (ssMWW-GST). Difference in menadione survival ratio between MTC cell lines versus the others was assessed using two-sided t-test, unequal variance.
SPHINKS algorithm
We implemented SPHINKS, a machine-learning method that generalizes unseen data from observed data using semi-supervised approaches applied in gene regulatory networks reconstruction75. SPHINKS creates an unbiased context-specific kinome network, leveraging kinases abundance from proteomics, substrate abundance from phospho-proteomics and experimentally validated kinase–substrate interactions available from PhosphoSitePlus30. The classifier, as a binary model, was trained to recognize relationships between abundance profiles of kinase–phosphosite pairs. A positive training set was defined as the set of known substrates of a specific kinase. This represented the typical setting where a learner has access only to positive and unlabeled data (positive unlabeled)75, with high imbalance between positive and unlabeled examples. We combined easyensamble76 and bootstrap aggregating machine-learning ensemble meta-algorithm (bagging)77 to integrate several SVM classifiers trained on different instances of the negative set (Extended Data Fig. 4a). An SVM classifier was trained on the validated interactions (positive training set) and a subset of randomly selected unknown interactions (negative set). Each training example represents an interaction and a training matrix is formed juxtaposing kinase’s protein and substrate’s phospho-protein abundance on a set of corresponding cases, with examples along the rows. Using the matrix of all possible kinase–substrate pairs we obtained a score (between 0 and 1), representing the probability for each phosphosite to be a kinase substrate according to the classifier. As the randomly derived negative set may contain potential substrates, to improve the accuracy of the prediction, we applied the bagging, repeating the training/prediction steps 100 times using random sampling of the negative set (keeping the positive fixed). SPHINKS scores were derived as the average SVM score from all iterations. To create a set of predicted substrates (SOPS) for each kinase (a list of predicted kinase–substrate interactions), we selected interactions whose SPHINKS score was above the value for which at least 50% of the known interactions were retained and the Spearman’s correlation between kinase and phospho-substrate was positive.
Identification of subtype-specific master kinases
We applied the method described previously10 with modifications. The activity of an MK was defined as the quantification of the activation of its substrate program in each sample Xi (i = 1,…,85). We binned all substrates into 25 bins according to their average abundance across all samples. For each MK, we defined {s1,…,sK} the substrates in the SOPS of MK. We randomly extracted a set of n = 100 control substrates for each sk from the corresponding bin, {c1,…,c100K}. Thus, the control substrate set has a distribution of abundance levels comparable to that of SOPS, while being 100-fold larger. The activity of the MK in the sample Xi was computed as:
where ωsk and ωcj are the SPHINKS scores of the kth substrate or jth control substrate of the MK, respectively; and are the abundances of sk or cj in the ith sample, respectively. If Act (Xi, MK) > 0, the MK is activated in the ith sample, if Act (Xi, MK) < 0, the MK is inversely activated and if Act (Xi, MK) ≈ 0, it is deactivated.
We selected MKs that showed a significant difference in activity in one subtype compared to the others using MWW test (effect size > 0.3 and P < 0.01). For GBM, subtype-specific MKs were mapped on a kinome tree using KinMap78.
Benchmarking of SPHINKS
Impact of missing values and imputation algorithm
To establish how the SPHINKS prediction of kinase–phospho-substrate interactions degrades as the level of imputation increases, we performed a set of simulations in a controlled setting where we could have a gold standard. From the CPTAC-GBM un-imputed phospho-proteomic data, we selected sites with no missing values (n = 7,302) as input for SPHINKS and generated a kinase–phosphosite interactome to be used as a gold standard. To simulate missing values, we generated new phospho-proteomic datasets by randomly replacing predefined ratios of phosphosites with missing values (r = 10%, 25% and 50%) and then imputed using DreamAI. We applied SPHINKS to predict the networks on the imputed matrices and compared them with the one reconstructed from the un-imputed matrix. The AUC from the ROC curve was computed as a measure of accuracy.
Validation of the predictions of kinase–phospho-substrate interaction
To evaluate SPHINKS performance in the prediction of kinase–substrate interactions, we performed a tenfold cross-validation analysis by randomly dividing the validated interactions from PhosphoSitePlus into ten subsets for training and testing. The workflow for each fold is as follows:
We trained the SVM using the training subsets (positive training set) plus a random selection of unknown interactions (negative training set).
As test set, we used the test subset and a randomly selection of unknown interactions, completely independent from the negative training set and derived the scores using the SVM classifier from step 1.
We derived the SPHINKS scores by applying the bagging approach as described before, repeating step 1 and 2 100 times.
We compared the SPHINKS scores with the test set and derived the AUC.
Validation of the kinase activity estimate
To evaluate how much different levels of interaction misclassifications affect the SPHINKS kinase activity, we randomly perturbed the SPHINKS network, as follows:
From the predicted kinase–substrate interactome, we generated a set of perturbations of interactions by replacing a predetermined percentage of phospho-substrates corresponding to P(percentage) = bottom 5%, 10%, 15%, 20% and 50% of the SPHINKS scores with random phosphosites.
For each percentage, we randomly generated n = 100 runs of perturbed networks.
For each percentage and run, we derived the SPHINKS kinase activity for 154 kinases in 85 CPTAC-GBM samples.
For each percentage and run, we derived the MK Δ-activity as the difference (in percentage) between the kinase activity inferred using the original network (Act(MK)u) and the perturbed networks (Act(MK)P):
Average for each kinase across all runs or for each run across all kinases were shown at each ratio of perturbation.
Comparison of the kinase activity inferred by SPHINKS and other methods
We considered two recently reported approaches, KSEA31 and KEA3 (ref. 32).
We used a dataset reporting the downstream changes in phospho-protein abundance after perturbations of upstream kinase by stimulators or inhibitors33,34, bringing together 24 studies encompassing 103 kinase-perturbation annotations (gold standard) for 30 kinases and 61,181 phosphosites. We employed a metric defined as ‘top-k-hit’ (Phit(k)), which focuses on the top k kinase predictions, as described34, with k = 10. To compare the kinase activity estimate among methods, for SPHINKS we considered only the validated interactions.
Additionally, we evaluated whether other approaches could identify the GBM subtype-specific kinases uncovered by SPHINKS. We applied each method on the CPTAC-GBM dataset and for each subtype derived the ranking of 129 kinases included in all five methods: (1) for SPHINKS, kinases were ranked according to the MWW score; (2) for KSEA PhosphoSitePlus and KSEA PhosphoSitePlus + NetworKIN, kinases were ranked based on the KSEA-derived z score31 for each subtype compared to the others; and (3) for KEA3, kinases were ranked based on the MeanRank or TopRank32 for each subtype (considering the highest 300 differentially phosphorylated proteins). For each kinase, we derived the Δ-rank as the difference in ranks between SPHINKS and any other approach (Δ-rank < 0, the rank of SPHINKS is lower, indicating higher kinase activity; Δ-rank > 0 indicates the opposite).
Processing and library preparation of the in-house GBM IDH wild-type cohort
The cohort is composed of 178 FFPE IDH wild-type GBM samples, 45 of which had matched frozen specimens. RNA was extracted using the Maxwell Rapid Sample Concentrator Instrument (Promega) and Maxwell RSC simplyRNA Tissue Kit (Promega, AS1340) for frozen samples or Maxwell RSC RNA FFPE kit (Promega, AS1440) for FFPE specimens. RNA extracted from both tissues was analyzed using the same workflow. Complementary DNA libraries were prepared with QuantSeq 3′ mRNA-Seq Library Prep kit FWD (Lexogen, 015). In brief, libraries were prepared with oligo-dT priming, with no previous poly(A) enrichment or ribosomal RNA depletion required. After first-strand synthesis, second-strand synthesis was initiated by random priming and Illumina-specific linker sequences were introduced. The resulting double-stranded cDNA was purified with magnetic beads and the library was then amplified, introducing the sequences required for cluster generation. Illumina libraries were multiplexed compatibly with single-end sequencing and sequenced on the Illumina HiSeq platform (100-bp single end). Sequencing data quality and pre-processing was as described5.
Development of the probabilistic classification tool for IDH wild-type GBM
We used 506 tumors from the TCGA-GBM profiled by Agilent as training set as these tumors were assigned to each functional subtypes based on orthogonal validation across multiple platforms including fCNVs, somatic mutations, DNA methylation and miRNA gene signatures5. The standardized expression of all genes from the subtype-specific signatures was used to train a multinomial regression model with lasso penalty using glmnet (α = 1, family = ‘multinomial’)79. We applied a tenfold cross-validation to select the best model with the lowest cross-validation error based on the misclassification error as loss measure. As a test set (ground truth), we considered two GBM IDH wild-type RNA-seq datasets:
TCGA-GBM cohort (n = 127) classified according to the subtyping of the matched Agilent expression tumors (ground truth);
CPTAC-GBM cohort (n = 85) classified in functional subtypes (ground truth) as described in this manuscript and orthogonally validated by multi-omics analyses (global proteomics, phospho-proteomics, lipidomics, metabolomics and acetylomics).
We classified the test samples if the fitted probability of a particular subtype was the highest and the sample showed a simplicity score above 0.35. The simplicity score was computed as the difference between the highest fitted probability (dominant subtype) and the mean of the other subtypes (non-dominant). We classified 80% of the TCGA and 79% of the CPTAC cohorts.
For the FFPE model, we used a similar approach with some modifications. We generated RNA-seq data from FFPE of 178 IDH wild-type GBM, 45 of which were also independently sequenced from matched frozen specimens (Supplementary Table 12). To identify genes whose expression in FFPE is consistent with the corresponding frozen specimens, we calculated correlation of expression between the 45 matched frozen and FFPE samples and retained only genes with Spearman’s correlation > 0.22 (4,668 genes). Independently, we classified the 45 fresh frozen samples’ extracted RNA to each subtype on the basis of the highest NES (ssMWW-GST) using the functional subtypes signatures5. Using the classification of the frozen samples as a ‘gold standard’, we derived FFPE-specific subtype-specific signatures on the FFPE expression matrix (50 highest genes from each ranked list, MWW test). As described for the frozen model, we trained a multinomial regression model on TCGA Agilent cohort using the FFPE-specific gene signatures and applied cross-validation to select the best model. The remaining 133 samples that lacked RNA-seq data from frozen specimens and had not been used to define the FFPE-specific signatures were classified if the fitted probability of a particular subtype was the highest and the simplicity score was above 0.25. We classified 73% of these tumors.
We performed an independent analysis to obtain an unbiased subtype assignment of the FFPE samples. FFPE-specific gene signatures were used to inform consensus clustering on the Euclidean distance matrix of all 178 FFPE-derived RNA-seq (10,000 random samplings, 70% of samples, Ward linkage, k = 4 clusters). We then labeled all samples by assigning each individual cluster to each subtype using the classification of the 45 matched frozen samples as ‘anchors’. We found 91% concordance in the classification of the matched frozen and FFPE-derived RNA-seq (41 out of 45). Finally, the unbiased label assignments of 133 unmatched FFPE samples were used to evaluate the prediction abilities of the classifier.
Association of GBM functional subtypes with clinical and radiomic features
Clinical data for TCGA-GBM patients were downloaded using TCGAbiolinks. Demographic characteristics were available for 503 GBM classified according to pathway-based classification. Patients were segregated in three age groups: 10–40, 40–65 and > 65 years. Quantification of radiomic features were available for 88 preoperative multimodal MRIs of TCGA-GBM from TCIA. For tumor location, patients were segregated in high or low group if more/less than 50% of the tumor was detected in the specific location, respectively. Univariate logistic regression analysis was performed to assess the association between demographic or radiomic features and functional subtypes/axis. Radiologist-made assessments (proportion of necrosis and edema) from TCGA (n = 63 GBM with available pathway-based assignment) were retrieved from elsewhere12. The proportion of DWM invasion available through TCIA was obtained by the integration of data published previously13 and REMBRANDT (n = 54). Quantitative radiomic features (n = 175) from 88 GBM were selected from TCIA as described80. We performed differential analysis of radiomic data in each subtype compared to the others (FC > 0.3, P < 0.05; two-sided MWW test). Association between functional subtypes and radiomic subgroups from unsupervised clustering was assessed by chi-squared test.
Cell culture
PDOs were cultured and tested as described5. Human cell lines were HEK293T (ATCC CRL-11268). Cells were cultured in DMEM supplemented with 10% FBS (Sigma). Cells were transfected using Lipofectamine 2000 (Invitrogen) or the calcium phosphate method. Lentiviral infection was performed as described10. Short hairpin RNA (shRNA) sequences (Sigma) for PKCδ are:
PRKCD shRNA 1 (TRCN0000010193): GGCCGCTTTGAACTCTACCGT;
PRKCD shRNA 2 (TRCN0000379731): CATTACTTGAATGTAGTTATC;
Cell growth and clonogenic assay
Time course analysis of the cellular growth of shPRKCD or empty vector-transduced PDOs was performed by plating 4,500 cells per well in 96-well plates. Viability was determined using CellTiterGlo assay reagent (Promega, G7570) and the GloMax-Multi+ Microplate Multimode Reader (Promega). For clonogenic assay of PDOs treated with BJE6-106, 1,500 cells were plated in six-well plates. Cells were fixed in methanol and stained with crystal violet after 2 weeks. Colonies with more than 50 cells were scored. Data are mean ± s.d. (n = 3 biological replicates). Experiments were repeated twice.
Intracellular glucose uptake and triacylglyceride accumulation
Measurement of the rate of glucose uptake and triacylglyceride accumulation in shPRKCD and control infected GPM PDO cells were performed as described elsewhere5.
Compound treatment
Cells were plated in 130 μl in opaque white 96-well plates. At 24 h later, cells were treated with serial dilutions of compounds as indicated for 72 h. Viability was determined as described5. For IR–drug combination treatment, PDOs were plated in 96-well plates. Cells were treated 24 h later with serial dilutions of M3814 and exposed 2 h later to IR (2, 4, 8 Gy at 0.7 Gy min−1) from a 137Cs source (GammaCell 40 irradiator, Teratronics). Mock-treated cells were cultured in parallel. Viability was determined 96 h later as described above. Clonogenic assays for the evaluation of IR–drug combination were performed in three independent 96-well plates for treatment group. The number of wells containing PDO spheres was scored and normalized to untreated cells.
Immunofluorescence analysis of γH2AX foci
Cells were fixed with 4% paraformaldehyde, permeabilized with cold methanol for 90 s at 4 °C and blocked with 5% BSA, 0.05% Triton X-100 in PBS for 30 min. Cells were exposed to primary antibody phospho-H2AX 1:500 dilution (Ser139, CST, 2577) for 1 h at room temperature followed by Cy3-conjugated anti-rabbit (Invitrogen, A10520) for 1 h at room temperature. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (Sigma). Images were acquired using a Nikon Ti Eclipse inverted microscope for spinning-disk confocal microscopy equipped with a Plan Apochromat ×60 oil/1.4 NA DIC objective. γH2AX foci in individual nuclei were scored by ImageJ (NIH) with in-built find Maxima > Prominence > Point Selection plug-in. Nuclei from at least ten random images were included in the analysis of each treatment group.
Western blot
Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP40, 0.5% sodium dexoycholate, 0.1% sodium dodecyl sulfate, 1.5 mM Na3VO4, 50 mM sodium fluoride, 10 mM sodium pyrophosphate, 10 mM β-glycerol phosphate and EDTA-free protease inhibitor cocktail; Roche). Lysates were briefly sonicated, cleared by centrifugation, separated by SDS–PAGE and transferred to polyvinylidene difluoride membrane. Membranes were probed with primary antibodies overnight at 4 °C: p-DNA-PK (Ser-2056, CST, 68716), DNA-PK (CST, 38168), p-NBS1 (Ser-343, CST, 3001), NBS1 (CST, 14956), p-KAP1 (Ser-824, Abcam, ab133440), KAP1 (Abcam, ab109287), p-CHK1 (Ser317, CST, 12302), CHK1 (CST, 2360), p-PKCδ (Tyr-311, CST, 2055), PKCδ (Abcam, ab182126), PKCδ (CST, 9616), p-STAT3 (Tyr705, CST, 9145), STAT3 (CST, 4904), p-AKT (Ser-473, CST, 4060), p-AKT (Thr308, CST, 13038), AKT (CST, 4691), p-ERK1/2 (Thr202/Tyr204, CST, 4370), ERK1/2 (CST, 9102), GAPDH (Abcam, ab9484), Vinculin (Sigma, V9131) and β-actin (Sigma, A5441). Secondary horseradish peroxidase-conjugated antibodies, anti-mouse (Invitrogen, 31438) or anti-rabbit (Invitrogen, 31458) were used, and either Enhanced ChemiLuminescence (Amersham, RPN2209) or Super Signal West Femto (Thermo Scientific, 34095) was used for detection. Dilution of all primary antibodies was 1:1,000 except GAPDH, vinculin and β-actin (1:10,000). Dilution of secondary antibodies was 1:10,000.
Statistics and reproducibility
In general, at least two independent experiments were performed with similar results. Experiments included a minimum of three replicates as specified in figure legends. No statistical methods were used to predetermine sample size. Data distribution was assumed to be normal but this was not formally tested. No data were excluded from the analyses; the experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Comparisons between two groups were analyzed by two-tailed t-test, unequal variance or the MWW test. Results in graphs are expressed as mean ± s.d. or mean ± s.e.m. as indicated in figure legends. Box plots span the first to third quartiles and whiskers show 1.5× interquartile range. All statistical analyses were performed using GraphPad Prism software v.8.0 to obtain P values.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Supplementary information
Acknowledgements
This work was supported by National Institutes of Health grant nos. U54CA193313, R01CA239721 and R01CA268592 (to A.L.); U54CA193313, R01CA190891, R01CA268592, R01CA239698 and R35CA253183; NCI P30 Supplement GBM CARE-HOPE; the Chemotherapy Foundation (to A.I.); and the Italian Association for Cancer Research Project IDs 21846 (IG) and 21073 (5 per mille) (to M.C.). S.M. is recipient of a fellowship from the Italian Association for Cancer Research. INCa-DGOS-INSERM_12560 (SiRIC CURAMUS) and the Ligue Nationale contre le Cancer (LNCC; Equipe labellisée) to M.S.
Extended data
Source data
Author contributions
A.L. and A.I. conceived and coordinated the studies and provided overall supervision. S.M. and L.G. developed and performed bioinformatics analyses of multi-omics data, with the assistance of F.D. and M.C.; Y.T.O. and H.M. performed cell, molecular biology and metabolic assays, with the collaboration of R.D.N.; M.S., F.B., A.L.D.S., J.L., A.P. and J.N.S. provided GBM samples and data. L.G., S.M. and M.C. wrote the computational sections. A.L. and A.I. wrote, edited and revised the manuscript with input from all authors.
Peer review
Peer review information
Nature Cancer thanks Bing Zhao and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Data availability
RNA-seq expression data of the 178 FFPE-derived and 45 frozen GBM IDH wild-type are available at Synapse (http://synapse.org; accession no. syn27042663). Previously published multi-omics data from CPTAC that were re-analyzed here are available from elswhere6,46–48. The human GBM transcriptomic, genomic, methylation and clinical data, BRCA and LUSC transcriptomic and clinical data were derived from the TCGA Research Network (http://cancergenome.nih.gov/) using TCGAbiolinks. BRCA transcriptomic data from METABRIC has been derived from elsewhere63. MNP-GBM methylation data were derived from the Gene Expression Omnibus (accession no. GSE90496). Source data have been provided as Source Data files. All other data supporting the findings of this study are available from the corresponding author on reasonable request.
Code availability
The source code used for SPHINKS and the GBM-specific kinome phosphorylome network are available at GitHub at https://github.com/miccec/MAKINA. The Shiny app of the frozen and FFPE classification tools is available at https://lucgar88.shinyapps.io/GBMclassifier.
Competing interests
A.L. and A.I. are inventors of a biomarker technology that has been licensed to QIAGEN. A.I. received sponsored research funding from AstraZeneca and Taiho Pharmaceutical and has served as a paid consultant/advisor to AIMEDBIO. A.L. received sponsored research funding from Celgene. A.L. and A.I. are inventors of a patent application based on this work. All other authors declare no competing interests.
Footnotes
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
These authors contributed equally: Simona Migliozzi, Young Taek Oh, Mohammad Hasanain, Luciano Garofano.
These authors jointly supervised this work: Anna Lasorella, Antonio Iavarone.
Contributor Information
Anna Lasorella, Email: axl4252@miami.edu.
Antonio Iavarone, Email: axi435@miami.edu.
Extended data
is available for this paper at 10.1038/s43018-022-00510-x.
Supplementary information
The online version contains supplementary material available at 10.1038/s43018-022-00510-x.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
RNA-seq expression data of the 178 FFPE-derived and 45 frozen GBM IDH wild-type are available at Synapse (http://synapse.org; accession no. syn27042663). Previously published multi-omics data from CPTAC that were re-analyzed here are available from elswhere6,46–48. The human GBM transcriptomic, genomic, methylation and clinical data, BRCA and LUSC transcriptomic and clinical data were derived from the TCGA Research Network (http://cancergenome.nih.gov/) using TCGAbiolinks. BRCA transcriptomic data from METABRIC has been derived from elsewhere63. MNP-GBM methylation data were derived from the Gene Expression Omnibus (accession no. GSE90496). Source data have been provided as Source Data files. All other data supporting the findings of this study are available from the corresponding author on reasonable request.
The source code used for SPHINKS and the GBM-specific kinome phosphorylome network are available at GitHub at https://github.com/miccec/MAKINA. The Shiny app of the frozen and FFPE classification tools is available at https://lucgar88.shinyapps.io/GBMclassifier.