TABLE 1.
Glycoprotein mutations acquired in passaged virus isolates
| Passaged virus | Lineage: | A | B | C | D |
|---|---|---|---|---|---|
| HSV-SaHVgBpass | Passage # at sequencing: | Passage 20 | Passage 20 | Passage 20 | Passage 20 |
| Glycoprotein mutations: | gH-H789Y a | gH-H789Y | gH-H789Y | gH-H789Y | |
| gC-R228L | gC-R145W | gC-R147L b | gC-R145W | ||
| gC-Y242C | |||||
| gB3Apass | Passage # at sequencing: | Passage 26 | Passage 29 | Passage 29 | Passage 29 |
| Glycoprotein mutations: | gH-S830N | gH-S830N | gH-G104D | gH-S830N | |
| gC-L204F | gB-M742T | gB-A855V | gB-S392F | ||
| gC-C286S | gC-P225L | gG-E83stop | gB-V517I | ||
| gM-Y407stop | gI-K344extc | gD-P219L | |||
| gC-R228W | |||||
| gI-K344extc | |||||
| ΔgB/gB3Apass | Passage # at sequencing: | Passage 6 | Passage 6 | Passage 6 | Passage 13 |
| Glycoprotein mutations: | gH-S830N | gH-W30L | gH-L72P | gH-S830N | |
| gH-T76M | gH-W835ext d | gD-Q203H | gH-W835ext d | ||
| gD-Q203H | gE-V269M | ||||
| gC-S459N |
a
The gH mutations examined in this study are shown in bold.
b
Mutations in italics were detected in combination with WT sequence at that position (i.e., mixed sequence). Virus stocks were not plaque purified prior to sequencing.
c
Cytosine insertion in gI-T341 codon resulted in a frameshift mutation at residue K344. gI-K344ext residues after P343: QIPAPVVTHTNALPDGHRRRVGARGGGWASDAPRGPHDIHPNASPVGIGPRPLAGGTT.
d
Adenine insertion in gH-W835 codon resulted in a frameshift mutation that extends the gH CT. Full CT of gH-W835ext: KVLRTSVPFFLETRIKGAWLRPFIRPTE.