Higher doses of tisagenlecleucel are associated with improved outcomes: a report from the pediatric real-world CAR consortium

Key Points

• •Currently, patients receive a single dose of tisagenlecleucel across a wide dose range.
• •Clinicians could choose to give a higher dose of tisagenlecleucel upfront, within the approved dose range, to optimize response.

Visual Abstract

Abstract

Remarkable complete response rates have been shown with tisagenlecleucel, a chimeric antigen receptor (CAR) T-cell therapy targeting CD19, in patients up to age 26 years with refractory/relapsed B-cell acute lymphoblastic leukemia; it is US Food and Drug Administration approved for this indication. Currently, patients receive a single dose of tisagenlecleucel across a wide dose range of 0.2 to 5.0 × 10⁶ and 0.1 to 2.5 × 10⁸ CAR T cells per kg for patients ≤50 and >50 kg, respectively. The effect of cell dose on survival and remission is not yet well established. Our primary goal was to determine if CAR T-cell dose affects overall survival (OS), event-free survival (EFS), or relapse-free-survival (RFS) in tisagenlecleucel recipients. Retrospective data were collected from Pediatric Real World CAR Consortium member institutions and included 185 patients infused with commercial tisagenlecleucel. The median dose of viable transduced CAR T cells was 1.7 × 10⁶ CAR T cells per kg. To assess the impact of cell dose, we divided responders into dose quartiles: 0.134 to 1.300 × 10⁶ (n = 48 [27%]), 1.301 to 1.700 × 10⁶ (n = 46 [26%]), 1.701 to 2.400 × 10⁶ (n = 43 [24%]), and 2.401 to 5.100 × 10⁶ (n = 43 [24%]). OS, EFS, and RFS were improved in patients who received higher doses of tisagenlecleucel (P = .031, .0079, and .0045, respectively). Higher doses of tisagenlecleucel were not associated with increased toxicity. Because the current tisagenlecleucel package insert dose range remains broad, this work has implications in regard to targeting higher cell doses, within the approved dose range, to optimize patients’ potential for long-standing remission.

Introduction

Remarkable complete response rates of 70% to 90% have been shown with chimeric antigen receptor (CAR) T-cell therapy in patients with refractory B-cell acute lymphoblastic leukemia (B-ALL).^1,2 Tisagenlecleucel (Kymriah) is a CD19-directed CAR T-cell product and was approved by the US Food and Drug Administration for treatment of relapsed/refractory B- ALL in children and young adults.

In the pivotal ELIANA trial,² which led to commercial approval, there was a target dose of transduced cells of 2.0 to 5.0 × 10⁶ CAR-transduced viable T cells per kg of body weight (for patients ≤50 kg) and 1.0 to 2.5 × 10⁸ T cells (for patients >50 kg) as a single infusion. However, patients could be infused according to the following dose range if safety release criteria were met: 0.2 to 5.0 × 10⁶ T cells per kg of body weight (for patients ≤50 kg) and 0.1 to 2.5 × 10⁸ T cells (for patients >50 kg). These latter doses are the current dose ranges used for commercial tisagenlecleucel infusion. To date, it is unclear whether cell dose influences CAR T-cell outcomes. It is of great interest to determine CAR T-cell properties optimized for efficacy and toxicity mitigation. The goal of this study was to use data from the Pediatric Real World CAR Consortium (PRWCC) to analyze the effect of infused CAR T-cell dose on efficacy and toxicity.

Methods

Study design

We used data extracted from the PRWCC across 15 pediatric oncology centers delivering commercial tisagenlecleucel. Centers obtained institutional review board approval, and deidentified data were captured using a Health Insurance Portability and Accountability Act–compliant REDCap data capture tool. The study was conducted according to the Declaration of Helsinki. Patients with relapsed/refractory ALL who underwent leukapheresis shipment to Novartis (Hanover, NJ) for commercial tisagenlecleucel manufacturing from 30 August 2017 to 6 March 2020 were included. Infused cell dose was recorded by institution in accordance with the Novartis certificate of analysis. For patients >50 kg, where flat dosing was used, dose was divided by patient kg of body weight to allow cross-cohort comparative analysis. Additional exploratory analysis was preformed, restricted to patients ≤50 kg to eliminate effect of dose capping in patients >50 kg.

Statistical analysis

All infused patients with dose information available were included (n = 180 of 185 infused). Of note, there were no out-of-specification products because of inadequate cell dose in this cohort. Four dose groups were defined using dose quartiles. Clinical and demographic characteristics were summarized by dose group using median and interquartile range for continuous variables and frequency and percentage for categorical variables. For comparisons of baseline characteristics by dose, dose group was treated as ordinal. For continuous variables, association with dose was tested with linear regression, with dose groups assigned scores of 1 to 4. For ordinal and binary variables, association with dose was tested with a χ² test of linear-by-linear association.³ Association between dose and race/ethnicity was tested with a χ² test with dose treated as ordinal and race/ethnicity as nominal. Tests of linear-by-linear association were also used to assess the relationship between dose group and cytokine release syndrome (CRS) and neurotoxicity (any grade and grade ≥3) and day-28 response.

Overall survival (OS) was defined as time from infusion to death resulting from any cause. Event-free survival (EFS) was defined as time from infusion to the earliest of nonresponse, relapse, or death. Patients who did not respond were considered to have had an EFS event on day 28. OS and EFS were censored at last follow-up. Relapse-free survival (RFS) was defined only in responders (n = 154) and was defined as time from day 28 to relapse. Patients who died or underwent hematopoietic stem cell transplantation (HSCT) before relapse were censored at time of death/HSCT, and all other relapse-free patients were censored at last follow-up. OS, EFS, and RFS were summarized by dose group using Kaplan-Meier curves and compared using the log-rank test. Cox regression models were used to investigate associations between dose group and other clinical and demographic characteristics and OS, EFS, and RFS.

All statistical analyses was performed using R (version 4.0.2; R Foundation for Statistical Computing, Vienna, Austria) with the survival, coin, gtsummary, and survminer packages.^4,5 P values <.05 were considered significant, and all CIs are 95% CIs.

Results

We conducted a retrospective multi-institutional analysis across PRWCC centers (n = 15). All patients who were infused and evaluable with tisagenlecleucel and had a recorded cell dose (n = 180) were included. Distribution and frequency of cell doses are shown in Figure 1A. As shown, a majority of patients received a cell dose <2 × 10⁶ cells per kg. The median cell dose of this cohort was 1.7 × 10⁶ cells per kg.

Figure 1.

Dose distribution of tisagenlecleucel in 184 infused and evaluable patients. Frequency of dosing (A) and patient breakdown into quartiles (B).

To examine associations between cell dose, demographic characteristics, and outcome, we divided all infused patients into 4 groups based on dose quartiles (Figure 1B): 0.1341.300 × 10⁶ (D1; n = 48 [27%]), 1.301 to 1.700 × 10⁶ (D2; n = 46 [26%]), 1.701 to 2.400 × 10⁶ (D3; n = 43 [24%]), and 2.401 to 5.100 × 10⁶ (D4; n = 43 [24%]). Clinical and demographic characteristics are summarized across dose groups in Table 1. We found no significant difference between dose quartiles with regard to sex, race/ethnicity, number of prior relapses, indication of CAR T-cell infusion (refractory or relapse), number of lines of therapy before CAR T cells, prior CD19-directed therapy, prior SCT, preinfusion disease burden, or months from initial diagnosis to CAR T-cell infusion. Interestingly, patients who were younger both at initial ALL diagnosis and at CAR T-cell infusion had higher cell doses infused than older patients (P < .001 for both). More favorable baseline cytogenetic risk was associated with higher infused doses (5% of D1 had favorable cytogenetic risk vs 33% in D4; P = .002 for trend; 43 patients were missing initial cytogenetic risk). Higher infused dose was also associated with higher CAR T-cell viability (median viability was 86%, 88%, 88%, and 90% for lowest to highest dose group, respectively; P = .020 for trend) and higher percentage of CAR T cell⁺ viable cells (median percentage of CAR T cell⁺ viable cells was 11.6%, 14.1%, 16.0%, and 15.7% for lowest to highest dose group, respectively; P = .017 for trend).

Table 1.

Patient characteristics across dose cohorts

Characteristic	Dose, × 106 CAR T cells per kg				P∗
	0-1.3 n = 48	1.3-1.7 n = 46	1.7-2.4 n = 43	2.4-5.1 n = 43
Sex					.7
Female	19 (40)	15 (33)	22 (51)	16 (37)
Male	29 (60)	31 (67)	21 (49)	27 (63)
Age at diagnosis, y	13 (8-16)	8 (3-16)	6 (2-12)	5 (3-8)	<.001
Age at infusion, y	17 (13-20)	13 (9-18)	11 (6-16)	10 (7-13)	<.001
Race/ethnicity					.3
Asian	2 (4.3)	0 (0)	3 (7.1)	2 (4.8)
Black	3 (6.5)	3 (6.8)	0 (0)	1 (2.4)
Hispanic	18 (39)	21 (48)	16 (38)	12 (29)
>1 race	1 (2.2)	1 (2.3)	2 (4.8)	0 (0)
Non-Hispanic White	22 (48)	19 (43)	21 (50)	27 (64)
Unknown	2	2	1	1
Initial cytogenetic risk					.002
Favorable	2 (5.0)	4 (12)	7 (21)	10 (33)
Intermediate	15 (38)	12 (35)	12 (36)	10 (33)
Unfavorable	23 (57)	18 (53)	14 (42)	10 (33)
Unknown	8	12	10	13
N of relapses pre-CAR	1.50 (1.00-2.00)	1.00 (1.00-2.00)	1.00 (1.00-2.00)	1.00 (1.00-2.00)	>.9
Relapsed or refractory indication					.6
Refractory	8 (17)	10 (22)	7 (16)	6 (14)
Relapsed	40 (83)	36 (78)	36 (84)	37 (86)
N of additional lines of therapy pre-CAR	2.00 (2.00-3.00)	3.00 (1.00-3.75)	2.00 (1.00-3.00)	2.00 (1.50-3.00)	.3
Prior CD19-directed therapy	11 (23)	9 (20)	11 (26)	6 (14)	.4
Pre-CAR SCT	13 (27)	14 (30)	9 (21)	9 (21)	.3
Preinfusion disease burden overall					.9
High	25 (54)	23 (51)	21 (50)	21 (49)
Low	9 (20)	8 (18)	10 (24)	14 (33)
No detectable disease	12 (26)	14 (31)	11 (26)	8 (19)
Unknown	2	1	1	0
CAR viability, %	86 (83-92)	88 (83-93)	88 (85-92)	90 (86-94)	.020
Unknown	0	0	0	1
Determination of CAR expression (% CAR+viable cells)	11.6 (8.6-15.4)	14.1 (11.1-17.7)	16.0 (12.0-20.9)	15.7 (9.9-19.1)	.017
Unknown	1	0	1	2
Time from diagnosis to CAR T-cell infusion, mo	28 (17-58)	34 (12-54)	30 (12-56)	39 (12-64)	.8

Data are presented as n (%) or median (interquartile range). Association with dose was tested with linear regression, with dose groups assigned scores of 1-4. Association between dose and ordinal variables was tested with χ² test of linear-by-linear association. Association between dose and race/ethnicity was tested with χ² test, with dose treated as ordinal and race/ethnicity as nominal.

^∗Pearson χ² test; χ² test of linear-by-linear association; linear regression; χ² test of ordinal association.

OS

Analyses for OS included 180 participants; OS at 3 years was 58.9% (95% CI, 47.6-72.9). Among 132 patients who were alive at last follow-up, the median follow-up was 402.5 days (range, 33-863). OS by dose group is shown in Figure 2. Dosing was significantly associated with survival (log-rank P = .031). In univariable Cox models for OS (supplemental Table 1), higher dose was associated with improved survival (P = .026), and the magnitude of the effect increased across increasing dose groups (hazard ratio [HR], 0.68; 95% CI, 0.33-0.1.38 for D2 vs D1; HR, 0.53; 95% CI, 0.25-1.11 for D3 vs D1; and HR, 0.26; 95% CI, 0.10-0.69 for D4 vs D1). Interestingly, male patients had better OS compared with female patients (HR, 0.51; 95% CI, 0.29-0.91 for male vs female patients; P = .021). Another characteristic that was significantly associated with OS was the number of additional lines of therapy before CAR T-cell infusion, where more lines were associated with inferior outcomes (HR, 1.17; 95% CI, 1.01-1.35 for each additional line; P = .032), and patients with no detectable disease or low disease burden had improved outcomes compared with patients with high disease burden (HR, 0.18; 95% CI, 0.07-0.52 and HR, 0.30; 95% CI, 0.12-0.77, respectively; P < .001). In a multivariable Cox model, dose group and preinfusion disease burden remained significantly associated with OS (Table 2). Patients receiving higher CAR T-cell dosing had improved OS (HR, 0.70; 95% CI, 0.34-1.46 for D2 vs D1; HR, 0.40; 95% CI, 0.18-0.87 for D3 vs D1; and HR, 0.22; 95% CI, 0.08-0.61 for D4 vs D1; P = .008). Independent of cell dose, patients with no detectable disease or low disease burden also had improved OS (HR, 0.18; 95% CI, 0.06-0.5 and HR, 0.38; 95% CI, 0.15-0.99; P < .001). Exploratory analysis of OS across dose quartiles in a limited sample restricted to patients ≤50 kg, without dose capping, recapitulated dose effect on OS (P = .029; supplemental Figure 1).

Figure 2.

OS, EFS, and RFS across CAR T-cell dose quartiles. Higher doses of tisagenlecleucel resulted in improved OS (A), EFS (B), and RFS (C).

Table 2.

Multivariable analysis

Characteristic	OS			EFS			RFS
	HR	95% CI	P	HR	95% CI	P	HR	95% CI	P
Dose group, × 106CAR T cells per kg			.008			<.001			.002
0-1.3	—	—		—	—		—	—
1.3-1.7	0.7	0.34-1.46		0.51	0.29-0.88		0.4	0.19-0.82
1.7-2.4	0.4	0.18-0.87		0.4	0.22-0.73		0.43	0.2-0.89
2.4-5.1	0.22	0.08-0.61		0.24	0.12-0.49		0.18	0.07-0.49
Age at infusion, y	0.98	0.94-1.03	.4	0.97	0.94-1.00	.085	0.98	0.94-1.03	.4
Preinfusion disease burden overall			<.001			<.001			.004
High	—	—		—	—		—	—
Low	0.38	0.15-0.99		0.34	0.18-0.65		0.38	0.17-0.88
No detectable disease	0.18	0.06-0.50		0.29	0.16-0.52		0.38	0.19-0.74
Sex
Female	—	—		—	—		—	—
Male	0.56	0.31-1.02	.057	0.75	0.48-1.15	.2	0.69	0.4-1.21	.2

EFS

Across 180 patients analyzed for EFS, 88 experienced an EFS event. Among those who were alive and event free at last follow-up, the median follow-up was 402.5 days (range, 33-856). EFS by dose group is shown in Figure 2. Dose group was significantly associated with EFS (log-rank P = .0079). In the univariable analysis for EFS (supplemental Table 2), patients receiving the highest dose range had better EFS (HR, 0.33; 95% CI, 0.17-0.65 for highest vs lowest dose group; P = .009). Interestingly, male patients also had better EFS compared with female patients (HR, 0.66; 95% CI, 0.43-1.00; P = .049). Patients with no detectable disease or low disease burden had improved outcomes compared with those with high disease burden (HR, 0.34; 95% CI, 0.19-0.60 and HR, 0.31; 95% CI, 0.16-0.58, respectively; P < .001). Multivariable analysis of EFS demonstrated that patients infused with the highest dose range had improved EFS (HR, 0.24; 95% CI, 0.12-0.49 for D4 vs D1; P < .001). Younger age at infusion was also associated with improved EFS, although the effect was not statistically significant (HR, 0.97; 95% CI, 0.94-1.00 for each 1-year increase in age; P = .085). Independent of dose, patients with no detectable disease or low disease burden also had improved EFS (HR, 0.29; 95% CI, 0.16-0.52 and HR, 0.34; 95% CI, 0.18-0.65, respectively, compared with those with high disease burden; P < .001). Exploratory analysis of EFS across dose quartiles in a limited sample restricted to patients ≤50 kg, without dose capping, recapitulated dose effect on EFS (P = .0014; supplemental Figure 1).

RFS

Post-CAR HSCT was distributed without significant difference across dose quartiles (supplemental Table 3). RFS was analyzed across 154 responders, 54 of whom experienced post-CAR relapse without interval HSCT (supplemental Table 3). Among those who were relapse free at last follow-up, the median follow-up was 300 days (range, 33-856). RFS by dose group is shown in Figure 2. Dose group was significantly associated with RFS with or without censoring for transplantation (log-rank P = .0045 and .0019, respectively; supplemental Figure 2). Moreover, when patients were not censored for transplantation, RFS was also improved in those who received higher doses. In the univariable analysis for RFS (supplemental Table 4), patients receiving the highest dose range had improved RFS (HR, 0.24; 95% CI, 0.10-0.59; P = .0506). Male patients had improved RFS compared with female patients, although the difference was not statistically significant (HR, 0.60; 95% CI, 0.35-1.03; P = .066). Patients with no detectable disease or low disease burden had improved RFS compared with those with high disease burden (HR, 0.44; 95% CI, 0.23-0.85 and HR, 0.33; 95% CI, 0.15-0.75, respectively; P = .003). Multivariable analysis of RFS demonstrated that patients infused with the highest T-cell dose had improved RFS (HR, 0.18; 95% CI, 0.07-0.49; P = .002). RFS was additionally improved in patients with no detectable disease or low disease burden (HR, 0.38; 95% CI, 0.17-0.88 and HR, 0.43; 95% CI, 0.19-0.74, respectively; P = .004). Exploratory analysis of RFS across dose quartiles in a limited sample restricted to patients ≤50 kg, without dose capping, recapitulated dose effect on RFS (P = .0015; supplemental Figure 1).

Response rates and toxicity

We assessed the impact of cell dose on response rate at day 28 after CAR T-cell infusion. Response was defined as no evidence of morphologic leukemia in the bone marrow. As shown in Table 3, dose was not associated with initial response after CAR T-cell therapy (response rate was 83%, 89%, 88%, and 92% for the dose groups from lowest to highest, respectively; P = .2) and was not associated with the initial response rate in the <50 kg cohort (supplemental Table 5).

Table 3.

Response rates by dose group

Characteristic	Dose, × 106 CAR T cells per kg				P∗
	0-1.3 n = 48	1.3-1.7 n = 46	1.7-2.4 n = 43	2.4-5.1 n = 43
Responded	39 (83)	40 (89)	38 (88)	37 (92)	.2
Unknown	1	1	0	3

Data are given as n (%).

^∗χ² test of linear-by-linear association.

We additionally assessed the impact of CAR T-cell dose on the rate of CRS or neurologic toxicity. In this cohort, higher CAR T-cell dosing was not associated with an increased risk of developing CRS, neurotoxicity, or grade ≥3 adverse events (Table 4). Moreover, there was no difference in treatment with steroids or tocilizumab across quartiles.

Table 4.

Toxicity across dose quartiles

Characteristic	Dose, × 106 CAR T cells per kg				P∗
	0-1.3 n = 48	1.3-1.7 n = 46	1.7-2.4 n = 43	2.4-5.1 n = 43
Did patient develop CRS?	25 (52)	28 (61)	26 (60)	28 (65)	.2
Did patient experience neurotoxicity?	11 (23)	10 (22)	7 (16)	11 (26)	>.9
Unknown	1	1	0	1
Grade ≥3 CRS	13 (27)	10 (22)	8 (19)	6 (14)	.11
Unknown	0	1	0	0
Grade ≥3 neurotoxicity	2 (4.3)	4 (9.1)	1 (2.3)	4 (9.5)	.6
Unknown	1	2	0	1
Treatment of neurotoxicity (choice, steroids [systemic])					.2
Checked	2 (4.2)	3 (6.5)	1 (2.3)	6 (14)
Unchecked	46 (96)	43 (93)	42 (98)	37 (86)
Treatment of neurotoxicity (choice, IT steroids)
Unchecked	48 (100)	46 (100)	43 (100)	43 (100)
Treatment of neurotoxicity (choice, tocilizumab)					.6
Checked	2 (4.2)	2 (4.3)	0 (0)	2 (4.7)
Unchecked	46 (96)	44 (96)	43 (100)	41 (95)

Data are given as n (%).

IT, intrathecal.

^∗χ² test of linear-by-linear association; Fisher exact test.

Discussion

The goal of this study was to explore the impact of cell dose on outcome after tisagenlecleucel. Current dosing of tisagenlecleucel according to the manufacturer’s package insert dictates that a single dose of tisagenlecleucel contains 0.2 to 5.0 × 10⁶ CAR⁺ viable T cells per kg of body weight for patients ≤50 kg or 0.1 to 2.5 × 10⁸ CAR⁺ viable T cells for patients >50 kg, suspended in 1 to 3 patient-specific infusion bags for IV infusion. Therefore, there exists a wide dose range. Tisagenlecleucel products are occasionally manufactured in dosing aliquots per bag that would permit infusion of 1, 2, or more bags, while remaining within the recommended dose range. To interrogate tisagenlecleucel dose effect, we divided patients into dose quartiles using actual per-kg doses administered to patients receiving commercial tisagenlecleucel and demonstrated that higher CAR T-cell dosing was associated with improved OS, EFS, and RFS in multivariable models (all P < .05).

Preclinical experiments by Milone et al⁶ established that there is a threshold of CAR T cells required to achieve an antileukemic effect. In their model, T cells expressing CD19ζ or CD19-BB-ζ CAR T cells were administered to immune-deficient NOD-SCID-γ^−/− mice with B-ALL. They found a dose-dependent antileukemic effect in the mice treated with 5 or 20 × 10⁶ CAR T cells. In animals that received only 1 × 10⁶ CAR T cells, there was only a partial effect on leukemia clearance, supporting that a CAR T-cell dosing threshold is required to achieve leukemia eradication.

In the pivotal ELIANA study, patients received a median dose of 3.1 × 10⁶ CAR T cells per kg (range, 0.2-5.4 × 10⁶).² The median dose in this study across recipients of commercial tisagenlecleucel was 1.7 × 10⁶ CAR T cells per kg, which is substantially lower than the dosing described in the clinical trial; 76% of patients received ≤2.4 × 10⁶ cells per kg in our study. Lower numbers of CAR T cells were also observed by Pasquini et al⁷; a median dose of 2 × 10⁶ cells per kg was observed in their study for patients ≤50 kg with ALL. Numerous possibilities may account for this discrepant dosing, including manufacturing changes and scalability challenges with commercialization, types of patients receiving tisagenlecleucel, and/or additional unknown factors. Given these differences, we felt it was important to investigate posttisagenlecleucel outcomes based on cell dose.

In an evaluation of 79 patients with B-ALL from both the ENSIGN and ELIANA trials, clinical responses were seen in all evaluated dose ranges (patients ≤50 kg, 0.2-5.0 × 10⁶/kg; patients >50 kg, 0.1 to 2.5 × 10⁸ CAR⁺ viable T cells).⁸ Patients were also evaluated at certain time points for CAR T-cell expansion and persistence. In responding patients, they identified a median peak expansion of 31.6% CD3⁺ CAR⁺ cells (range, 1.10-84.9). In nonresponding patients, there was rapid clearance of the CAR⁺ T cells. Because of the retrospective nature of this study, CAR expression and CAR T-cell expansion were not evaluated. However, one could envision that higher doses of CAR T cells may result in greater persistence and better outcomes compared with lower doses, as shown in this study. This should be considered in future analyses of CAR-mediated dose response.

A recent study by Ayuk et al⁹ examined doses of axicabtagene ciloleucel prospectively in 21 patients who were being treated for aggressive B-cell lymphoma using polymerase chain reaction to determine peak CAR T-cell count in vivo. In this study of real-world patients, the median peak CAR T-cell count was 16.14 CAR T cells per μL. Patients with ≥16.14 cells per μL peak CAR T cells were considered strong expanders and were found to have improved day-30 objective responses compared with those who were not strong expanders (91% vs 40%; P = .02).

In a recent phase 1 study of KTE-X19 for adult patients with ALL, a dose of 1 × 10⁶ CAR T cells per kg dose had the most favorable risk/benefit ratio without compromising activity when combined with appropriate adverse event management.¹⁰ KTE-X19 is a CD28 CAR T-cell therapy containing a CD3z and CD28 costimulatory domain,^11,12 whereas tisagenlecleucel has a 4-1BB domain, which, in addition to the patient population, could account for the differences. Kymriah is approved for patients up to age 26 years, and the median age in the phase 1 study was 46 years (range, 18-77).

One concern with increasing CAR T-cell dosing is the possibility of increased rates of CRS and/or neurotoxicity. In the pivotal ELIANA trial,² 77% of patients had CRS. Neurologic toxicity was observed in 40% of patients. Data from multiple phase 2 studies of tisagenlecleucel for both B-ALL and adult diffuse large B-cell lymphoma were used to examine how dose is related to efficacy, safety, and exposure.¹³ In patients with B-ALL, logistic regression analyses showed that a higher CAR T-cell dose was associated with a higher probability of response.¹³ There was no increase in CRS incidence or severity across dose ranges, and patients achieved comparable early response rates independently of dose. In this study, increased doses were not associated with increased toxicity.

In a recent study by Pasquini et al,⁷ real-world outcomes of patients with ALL and non-Hodgkin lymphoma treated with tisagenlecleucel were evaluated using the Center for International Bone Marrow Transplant Registry database. In this evaluation, logistic regression was used to assess the association between best overall response and CAR T-cell dose infused. In patients with ALL with a weight of ≤50 or >50 kg, dose did not affect best overall response. Our study additionally analyzed survival outcomes (ie, OS, EFS, and RFS), with all 3 outcomes affected by cell dose, where higher cell dose was associated with significant improvement in OS, EFS, and RFS.

The dose groups compared in our current study were based on dose quartiles generated to interrogate patterns of association between dose and outcome, not to identify an optimal dose threshold or dose range. In the higher dose quartiles, improved cytogenetics were seen compared with lower dose quartiles, which potentially could have affected the ability to collect appropriate T cells for CAR T-cell generation and could also have served as a confounder in our data set. Therefore, the impact of cytogenetics on posttisagenlecleucel outcomes needs to be examined in additional studies.

Further research is warranted to elucidate the relationship between threshold dosing and post-CAR outcomes. Our results, however, indicate that higher tisagenlecleucel doses are associated with superior survival outcomes in the real-world setting and suggest further exploration of higher dosing strategies, within the safe range of tisagenlecleucel, in the clinical setting.

Conflict-of-interest disclosure: H.E.S. has served on an advisory committee and speaker’s bureau for Novartis. M.R.V. has served on an advisory committee for Novartis, has been a consultant for and is a current equity holder in Fate Therapeutics and Bmogen, and has been a consultant for UpToDate. C.L.P. has served on an advisory committee for Novartis. S.P.M. has served on an advisory committee for Novartis and Jazz Pharmaceuticals. G.D.M. served on the ELIANA trial steering committee and speaker’s bureau and has served as a consultant and received honoraria from Novartis. P.A.B. has served on an advisory committee for Novartis, Kite, Takeda, Janssen, Kura, Servier, and Jazz Pharmaceuticals. M.Q. has served as a consultant for Novartis and Mesoblast. M.H. has served on an advisory board for Sobi and Novartis. P.S. has served as a consultant for Takeda and Mesoblast. K.J.C. has served as a consultant for and received research funding from Novartis, has served as a consultant for Mesoblast, and has received research funding from Celgene. C.L.M. has served as a consultant for and is a current equity holder in Lyell Immunopharma and Apricity Health, has served as a consultant for NeoImmune Tech, Nektar Therapeutics, and Bristol Myers Squibb, and is a current equity holder in Allogene. T.W.L. reports consultancy relationships with Novartis, Cellectis, Bayer, Deciphera, Jumo Health, and Y396 mAbs Therapeutics and research funding from Pfizer, Novartis, and Bayer. The remaining authors declare no competing financial interests.