Due to a production error, we missed implementing change requests to correct typos, formatting, and to use the final version of Figures 5 , 8 .
Figure 5.
In vitro enzyme activity and specificity assays for yeast expressing PaDGAT1. PaDGAT1 with an N-terminal HA epitope tag was expressed in the H1246 yeast strain and was induced for 24 h. Microsomal fractions were prepared and incubated with radiolabeled fatty acyl-CoA and DAG. Saturated (palmitoyl-CoA, 16:0-CoA) and monounsaturated (oleoyl-CoA, 18:1-CoA) acyl donor substrates were tested. Representative chromatogram generated by Radio-TLC imaging scanner for negative control with only acyl-CoA substrate and no enzyme (A), and PaDGAT1 microsomes incubated with 16:0 or 18:0 acyl-CoA and diolein that resulted in TAG synthesis (B). Quantification of TAG product peaks revealed that PaDGAT1 prefers 18:1-CoA over 16:0-CoA (C). ***P<0.001.
Figure 8.
Quantification of LD accumulation and fatty acid profile in TAG in N. benthamiana leaves co-expressing PaDGAT1 and PaPDAT1. Confocal images of the accumulated LDs stained with Nile Red (green) in N. benthamiana leaves expressing control (+P19), DGAT1 (+P19), PDAT1 (+P19), and DGAT1 + PDAT1 (+P19) (A). The scale bar represents 20 μm. Quantification of total lipid extracted from the leaves and separation of TAG by TLC (B). Number of accumulated LDs per unit area (C) and their average size (area) (D). Fatty acid profile of extracted TAG from the TLC plates (E). Data represent mean ± SD of three independent experiments and different letters indicate significant differences (P < 0.05), as determined by ANOVA with Tukey’s post-test.
The correct figures for Figures 5 , 8 can be seen below.
The publisher apologizes for these mistakes. The original article has been updated.