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. 2023 Jan 13;33(2):138–149. doi: 10.1093/glycob/cwac082

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6 — Glycoconjugate production by 3 refactored pgl cluster variants in the A and B) wecA- waaL- W3110-derivative SDB1 and the C and D) wecA+ waaL- CLM24. A modified version of Hexahistidine tagged-AcrA with 7 glycosylation sequons was used as a PglB substrate acceptor protein. Glycoproteins were resolved by SDS-PAGE and were detected by immunoblot analysis probed with SBA lectin (green) and anti-6xHis antibody (red). Merged SBA lectin (green) and anti-6xHis (red) channels are shown in panels A and C; anti-6xHis only channel is shown in panels B and D.