From The Cover: Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa -- Habuchi et al. 102 (27): 9511 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Habuchi et al. 10.1073/pnas.0500489102.

Supporting Information

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Supporting Figure 6
Supporting Figure 7
Supporting Figure 8

Supporting Figure 6

Fig. 6. Steady-state spectra of Dronpa at pH 5.0; absorption spectrum (blue line), fluorescence spectrum excited at 488 nm (green line) and 390 nm (red line), and excitation spectrum detected at 540 nm (black line).

Supporting Figure 7 Fig. 7.
Absorption spectral change of Dronpa on irradiation. (a) Time evolution of the absorption spectrum of Dronpa (pH 5.0) on irradiation with a 488-nm laser (P = 10 mW/cm²). (b and c) Time evolution of the concentration of the deprotonated (b) and the photoswitched protonated (c) form on irradiation with a 488-nm laser. The solid lines show the fitting with a first-order kinetic model. (d) Time evolution of the absorption spectrum of the photoswitched Dronpa (488-nm irradiation) on irradiation with a 405-nm laser (P = 1.0 mW/cm²). (e and f) Time evolution of the concentration of the deprotonated (e) and the photoswitched protonated (f) form on irradiation with a 405-nm laser. The solid lines show the fitting with a first-order kinetic model.

Supporting Figure 8 Fig. 8.
Steady-state absorption (blue line), fluorescence (green line), and excitation spectra (black line) of photoswitched (488-nm irradiation) Dronpa at pH 7.4. The fluorescence spectrum was measured with 488-nm excitation. The excitation spectrum was measured with 540-nm detection.