Institution: NIH Library Sign In as Member / Individual

This Article Full Text Services Alert me to new issues of the journal Request Copyright Permission

Supporting information for Hansen et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.262485199

Supporting Information

Comparison of hits observed in crystallization experiments using standard sparse matrix crystallization screens (Hampton Research, Emerald Biostructures) for chip, microbatch, and hanging-drop formats. In hanging-drop experiments 1 µl of protein sample was combined with 1 µl of crystallizing agent on a glass cover slip and suspended over a well containing 0.5 ml of crystallizing agent. Microbatch experiments were conducted by combining 1 µl of protein sample with 1 µl of crystallizing agent under paraffin oil. Experiments were incubated at 25ºC for up to 3 weeks. A hit is defined as single crystals, plates, rods, or spherulites. Phase separation and precipitation were not counted as hits. Where crystals were observed, they were confirmed to be protein by staining with IZIT dye, harvesting, and crushing with a glass probe (the "click" test) and/or comparison with negative (no-protein) controls.