Supporting information for Comolli et al. (2002) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.262663599
Supporting Figure 8
Fig. 8. Stem-loop "P2b" consists of two ordered domains. (a) Ultraviolet absorbance melting curve of P2b (Lower) and its derivative (Upper). Melting experiments show typical curves corresponding to two well-defined transitions, with melting temperatures of 49ºC for one domain and 79ºC for the other domain. (b) The imino region in 1D NMR temperature profiles clearly shows two sets of peaks, one broadening above 35ºC and the other above 50ºC. They correspond to the loop and the stem, respectively, as confirmed by comparison with the UUCG tetraloop construct and by assignments.
