Regulation of allergic airway inflammation through Toll-like receptor 4-mediated modification of mast cell function -- Nigo et al. 103 (7): 2286 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Nigo et al. 10.1073/pnas.0510685103.

Supporting Figures

Files in this Data Supplement:

Supporting Figure 5
Supporting Figure 6
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Figure 10
Supporting Figure 11
Supporting Figure 12
Supporting Figure 13

Supporting Figure 5

Fig. 5. A representative photographic view of the bronchoalveolar lavage (BAL) fluid in each group shown in Fig. 1A. Wild type (+/+) (a–d) and W/W^v mice (e–h) were used. Arrows indicate eosinophils, determined by characteristic morphology.

Supporting Figure 6

Fig. 6. Wild type (+/+) and W/W^v mice were treated and challenged as in Fig. 1, and transcriptional levels of IL-4, IL-5, IL-13, and Eotxin-2 in the BAL fluid cells were determined by real-time RT-PCR analysis.

Supporting Figure 7

Fig. 7. Sections shown are representative of 10 lung sections per mouse from three mice in each group shown in Fig. 1B and are with hematoxylin and eosin (H.E.) staining; magnification, ´200.

Supporting Figure 8

Fig. 8. Sections shown are representative of 10 lung sections per mouse from three mice in each group shown in Fig. 1B and are with Luna staining; magnification, ´400. Arrows indicate eosinophils, determined by characteristic orange staining.

Supporting Figure 9

Fig. 9. Sections shown are representative of 10 lung sections per mouse from three mice in each group shown in Fig. 1B. The thickness (mm) of subepithelial fibrosis was as follows: 4.5 ± 0.7 (a), 3.0 ± 0.3 (b), 5.1 ± 1.3 (c), 9.9 ± 0.6 (d), 5.0 ± 1.1 (e), 5.1 ± 2.8 (f), 5.1 ± 1.3 (g), and 6.7 ± 1.0 (h).

Supporting Figure 10

Fig. 10. A representative staining pattern of cells in BAL fluid of Fig. 2A. W/W^v mice (a–d) and W/W^v mice reconstituted with wild type (+/+) BMMCs (e–h) were immunized with PBS (a and e), LPS (b and f), OVA (c and g), and OVA plus LPS (d and h). May–Giemsa stain; magnification, ×400.

Supporting Figure 11

Fig. 11. A representative staining pattern of cells in BAL fluid in each group shown in Fig. 2C with May–Giemsa stain; magnification, ´400.

Supporting Figure 12

Fig. 12. A representative staining pattern of cells in BAL fluid in each group shown in Fig. 2D with May–Giemsa stain; magnification, ´400.

Supporting Figure 13

Fig. 13. A representative staining pattern of cells in BAL fluid in each group shown in Fig. 2E with May–Giemsa stain; magnification, ´400.