Supporting Figures

Files in this Data Supplement:

Supporting Figure 7
Supporting Figure 8

Fig. 7. Amino acid sequence alignment of Miz-1 orthologs from diverse vertebrate species. The region involved in interaction with the Sleeping Beauty transposase is underlined.

Fig. 8. Human DNA sequences do not support Sleeping Beauty transposition. (A) DNA sequence alignment of the transposase binding sites within the Sleeping Beauty transposon and similar sequences found in human chromosomes. (B) Agarose gel showing products of excision PCR (8). The transposon donor constructs pT/neo, pT/neo-Chr8, and pT/neo-Chr15 were transfected into HeLa [IRES-SB] cells. PCR on pUC19 DNA was included as a positive control and molecular size marker. The amount of input DNA was visualized by PCR amplification of the neo gene.