Supporting Information

Files in this Data Supplement:

Supporting Table 2
Supporting Figure 5
Supporting Table 3
Supporting Text

Fig. 5. Unfolded population as a function of time for the bulk and each tether point. Curves represent folding at their respective values of T_f (noted in the figure) and for the tethered protein, h_t = s. Inset shows P_v(t) for tether point 34 (black), fit to an exponential function (red). As noted in the main text, tether point 34 is the only one that exhibits two-state folding; the others, including the bulk, fold with nonexponential kinetics.

Fig. 6. Probability of contact formation for the b-barrel protein tethered at either 2s or 5s above the surface. As with Fig. 2, the contact pairs closest to each tether point are indicated.