Supporting Information

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Supporting Figure 5
Supporting Table 2
Supporting Figure 6
Supporting Table 3

Fig. 5. Hair follicle developmental stages in embryos. Histology of embryonic back skin at different stages was shown. Hair germs are recognizable in E14.5 skin (arrows), but not yet seen at E13.5. Rudimentary hair germs are first recognizable at E16.5 in Ta skin. Maturing hair follicles are shown in E18.5 WT skin, along with nascent follicles in Ta skin.

Fig. 6. Additional molecular and histological phenotypes of mice and cells with variations in LTb and EDA pathways. Relative expression levels of Dkk4 (A) and LTb (B) during embryonic development of WT and Ta mice. (C) Phenotypes of mice that have received bone marrow transplants (see text). (D) Activation of p65 by EDAR overexpression. (A and B) Expression levels for Dkk4 and LTb genes declined sharply at E16.5 and thereafter in normal development and were somewhat further depressed in Tabby. Data from real-time PCR with three biological samples, normalized to GAPDH. (C) WT mice that received LTb-null marrow by transplantation show normal skin histology and hair shafts; LT-null mice that had received WT bone marrow showed a "skin spot" on the neck (Top; circled), thick collagen/thin fatty layers (Middle), and abnormal structure of hair shafts (Bottom). (D) Transient overexpression of EDAR in 293T cells induces up-regulation and nuclear translocation of p65 (Upper) but not of RelB (Lower).