Supporting information for Farris et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0230450100
Fig. 4.
Disruption of the IDE gene in mice. (A) Map of the VICTR48 gene trap vector and its site of integration in intron 1 of the mouse IDE gene. The targeting cassette is flanked by retroviral long terminal repeat (LTR) sequences and contains both a neomycin selectable marker flanked on the 5' end by a splice acceptor site and a fusion of the PGK promoter with the Bruton’s tyrosine kinase gene exon 1 flanked on the 3' end by a splice donor site. (B) OST sequence. This is identical to the mouse IDE sequence and paired with mouse IDE amino acid sequence from exon 2, which is located at the same site as the human IDE exon 2. The downstream OST sequence corresponds to IDE exon 3. (C) Sequence of the IDE intron 1/LTR junction. IDE intron 1 sequence is in bold. (D) Western blot analysis of IDE –/– and IDE WT mouse brain membrane fraction by using affinity-purified polyclonal antibody raised against amino acids 56–68 of human IDE (1).1. Vekrellis, K., Ye, Z., Qiu, W. Q., Walsh, D., Hartley, D., Chesneau, V., Rosner, M. R. & Selkoe, D. J. (2000) J. Neurosci. 20, 1657–1665.