Österberg et al. 10.1073/pnas.0604078103. |
Supporting Figure 5
Supporting Table 1
Fig. 5. Western blots of whole-cell lysates. Cells were grown overnight at 30°C in 5 ml of Ura medium in 50-ml Falcon tubes. Cells were harvested and resuspended in 100 ml of sample buffer (1), incubated at 56°C for at least 15 min, and 20 ml of the supernatant was loaded onto a 6.25% SDS gel (in lanes marked with *, 6.7 ml of the supernatant was loaded). In each gel, a constant amount of the purified membrane protein Yml125p and the E-PAGE (Invitrogen) protein standard marker were loaded (marked Standard and MW, respectively). The top two bands in the protein standard marker indicate 220 kDa and 120 kDa molecular mass. Gels were quantified in a Fuji LAS-1000Plus charge-coupled device camera light box by using IMAGE GAUGE 3.4. Numbers in parentheses indicate the number of bands that were included in quantification for each protein. Bands marked by # could not be quantified.
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