A connector of two-component regulatory systems promotes signal amplification and persistence of expression -- Kato et al. 104 (29): 12063 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Kato et al. 10.1073/pnas.0704462104.

Supporting Information

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SI Figure 6
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SI Figure 8
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SI Table 3
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SI Figure 6

Fig. 6. mRNA levels for the connector-mediated pathway (dark blue) and the direct regulation pathway (orange). (A) Experimental results used to generate Fig. 2B. Error bars correspond to standard deviation. (B) Steady-state curves generated computationally, using the models fitted to the data in Fig. 3A; these curves were used to produce the induction ratio curves shown in Fig. 3B (solid lines) by normalizing to the mRNA levels corresponding to [PhoP-P] = 0.192.

SI Figure 7

Fig. 7. The PmrD-FLAG protein has a longer half-life than the PmrD protein. Western blot analysis of whole-cell lysate (10 mg of total proteins) prepared from wild-type Salmonella enterica (14028s) and pmrD-FLAG (EG13654) strains after bacteria were grown in inducing conditions (i.e., 20 mM MgCl₂) for 2 h and then shaken for the indicated times after the addition of 200 mg/ml chloramphenicol. The value at time 0 corresponds to the time chloramphenicol was added.

SI Figure 8

Fig. 8. Turning off kinetics of pbgP transcription after activation of the PmrA/PmrB system. Relative pbgP mRNA levels in a pmrD S. enterica (EG11491) grown in inducing conditions (i.e., 100 mM FeCl₃) for 1 h and then grown for the indicated times after the addition of the Fe³⁺ chelator deferoxamine mesylate (300 mM). Time 0 corresponds to the time deferoxamine mesylate was added.