Fig. 4. Temperature variation of the period of glycolytic oscillations. The line is a quadratic polynomial fit to the experimental data; the slope at 25ºC is -3.7 s/ºC.

Fig. 5. Resonant forcing of oscillating yeast cells. A periodic forcing with Aca is started at t = 0 s and stopped again approximately 600 s later. The forcing period is 38 s, the same as the period of the unperturbed cells. The solid line is the fluorescence signal, and the dotted line is the forcing signal, i.e., the mixed flow Aca concentration. The forcing amplitude is 2.2 mM mixed flow Aca.

Fig. 6. Phase-shift experiments with increased glucose concentrations. Shown are amplitude (thin line) and phase (thick line) variations as response to180º phase shifts in the glucose forcing. The phase shifts are made at time t = 0 s. The average mixed flow glucose concentrations are 21 mM (a) and 33 mM (b), the mixed flow forcing amplitudes are 0.13 mM (a) and 0.19 mM (b), and we measured actual average glucose concentrations of 4.5 mM (a) and19 mM (b).

Fig. 7. Forcing with Aca at a non-natural frequency. Yeast cells were forced at a period of 36 s; 5% shorter than their natural period of 38 s. The resulting oscillations are torus oscillations. (a) Polar plot with the distance from the center indicating the amplitude of the forced yeast cells relative to that of the unforced, and the phase indicating the phase difference Dq = q_forcing - q_NADH between the Aca forcing and the fluorescence signal. The distance between the dots corresponds to a time interval of Dt = T = 36 s, and the largest dotted ring indicates the unforced amplitude. (b) Same data as time traces of the phase difference (thick line) and the relative amplitude (thin line).