Index of Online Supplemental Material for J. Cell Biol. 10.1083/jcb.200509075 Nayal et al. Figure S1Phospho- S273-paxillin antibody is specific and phospho- S273-paxillin levels are up-regulated during cell spreading.
Figure S2 Endogenous GIT1 distribution in pxl-/- MEFs and wild-type MEFs.
Figure S3 Phospho-S273-paxillin localizes specifically to small adhesions near the cell periphery.
Video 1 S273D-paxillin-GFP dynamics in a CHO-K1 cell.
Video 2 S273A-paxillin-GFP dynamics in a CHO-K1 cell.
Video 3 WT-paxillin-GFP dynamics in a CHO-K1 cell coexpressing KD-PAK.
Video 4 WT-paxillin-GFP dynamics in a CHO-K1 cell coexpressing CA-PAK.
Video 5 S273D-paxillin-GFP dynamics in a CHO-K1 cell coexpressing PIXΔGBD.
Video 6 S273D-paxillin-GFP dynamics in a CHO-K1 cell coexpressing PIXΔSH3.
Video 7 S273D-paxillin-GFP dynamics in a CHO-K1 cell coexpressing N17-Rac.
Video 8 WT-paxillin-GFP dynamics in a CHO-K1 cell coexpressing V12-Rac.
Video 9 TIRF video of a CHO-K1 cell expressing S273D-paxillin-GFP.
Video 10 TIRF video of a CHO-K1 cell expressing WT-paxillin-GFP.