Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200701078
Sun et al.

• Figure S1 Knockdown of PIPKIγ attenuated EGF-stimulated directional migration.

• Figure S2 Knockdown of PIPKIγ does not affect LPA- or SDF1α-stimulated cell migration.

• Figure S3 PIPKIγ is required for EGF-induced vinculin assembly into adhesions.

• Figure S4 The effect of PIPKIγ661, PIPKIγY634F, PIPKIγKD, or PIPKIγS645F on EGF-induced vinculin assembly to adhesions

• Video 1 Real-time video of polarized recruitment of GFP-talin to the leading edge in a gradient of EGF in control shRNA-transfected cells.

• Video 2 Real-time video of polarized recruitment of GFP-talin to the leading edge in a gradient of EGF in PIPKIγ668 shRNA-transfected cells.

• Video 3 Real-time video of polarized recruitment of GFP-talin to the leading edge in a gradient of EGF in mc-PIPKIγ661-expressing cells.

• Video 4 Real-time video of recruitment of mc-PIPKIγ661 to the leading edge in a gradient of EGF.

• Video 5 Real-time video of polarized recruitment of GFP-talin to the leading edge in a gradient of EGF in mc-PIPKIγY634F-expressing cells.

• Video 6 Real-time video of recruitment of mc-PIPKIY634F to the leading edge in a gradient of EGF.

• Video 7 Real-time video of polarized recruitment of GFP-talin to the leading edge in a gradient of EGF in mc-PIPKIγKD-expressing cells.

• Video 8 Real-time video of recruitment of mc-PIPKIγKD to the leading edge in a gradient of EGF.