|
Table 2. Primers used to amplify the 12S rRNA, tRNA valine, and 16S rRNA genes
Primer |
Xenopus laevis * |
Designation(1) |
Sequence (5'–3') |
MVZ59-L |
2158–2180 |
29 MVZ59 |
ATAGCACTGAAAAYGCTDAGATG |
12SJ-L |
2206–2225 |
34 12J-L |
AAAGGTTTGGTCCTAGCCTT |
12L1-L |
2475–2509 |
46 L1091 |
AAAAAGCTTCAAACTGGGATTAGATACCCCACTAT |
12SA-L |
2485–2509 |
47 12SA-L |
AAACTGGGATTAGATACCCCACTAT |
12SF-H |
2546–2566 |
54 12SF-H |
CTTGGCTCGTAGTTCCCTGGCG |
H29616-H |
2897–2916 |
68 12SB-H |
GAGGGTGACGGGCGGTGTGT |
H29616-L |
2897–2916 |
68 12SB-L |
ACACACCGCCCGTCACCCTC |
12SM-L |
2968–2989 |
— |
GGCAAGTCGTAACATGGTAAG |
12SK-H |
2975–2996 |
70 12SK-H |
TCCGGTGTGCTTACCATGTTACGA |
tRNAval-H |
3033–3059 |
73 tRNAval-H |
GGTGTAAGCGARAGGCTTTKGTTAAG |
16SH-H |
3282–3304 |
— |
GCTAGACCATGATGCAAAAGGTA |
16SC-L |
3623–3642 |
— |
GTRGGCCTAAAAGCAGCCAC |
16SDB-L |
3956–3976 |
88 16Sar-L |
CGCCTGTTTATCAAAAACAT |
16SA-H |
3956–3976 |
88 16Sar-H |
ATGTTTTTGATAAACAGGCG |
16SD-H |
4574–4574 |
96 16Sbr-H |
CTCCGGTCTGAACTCAGATCACGTAG |
L, light-chain primers; H, heavy-chain primers.
*Xenopus laevis mitochondrial genome location.
1. Goebel, A. M., Donnelly, J. M. & Atz, M. E. (1999) Mol. Phylogenet. Evol. 11, 163–199.