Supporting Figure 6
Fig. 6. Induction of intracellular signaling by highly cytokinergic IgE molecules (IgEs). (A) Bone marrow-derived cultured mast cells were incubated with 5 μg/ml of the indicated IgEs for 3 or 15 min. Cell lysates were subjected to immunoblotting with anti-phosphotyrosine (PY), anti-extracellular signal-regulated kinase (ERK), anti-phospho-extracellular signal-regulated kinase (pERK), anti-p38 (p38), anti-phospho-p38 (pp38), anti-Akt (Akt), or anti-phospho-Akt (pAkt). (B) Bone marrow-derived cultured mast cells were electroporated with the reporter plasmid tumor necrosis factor-α/luc 24 h before stimulation with 10 μg/ml of the indicated IgE. Luciferase activity was measured with cell lysates prepared after 8 h of stimulation.
