Fig. 7. CCI-779 treatment prevented nuclear hypertrophy of granule cells in the dentate gyrus. Pten mutant and control mice were treated with 7.5 mg/kg five times per week of CCI-779 from age P10-P28 and mice were euthanized at P28. Brain sections were stained with DAPI to visualize nuclei. In the dentate gyrus from 4-week-old control mice treated with vehicle (a) or CCI-779 (c), nuclei are similar in size. In mutant dentate gyrus from vehicle-treated mice (b), nuclei appear substantially larger than controls (a and c). Mutant cells also appear less bright as an equivalent amount of DNA was distributed over a larger area. Smaller, brighter nuclei similar to controls are also visible in the mutant dentate gyrus and, likely represent cells that did not delete Pten (arrow). CCI-779 treatment of mutant mice (d) prevented granule cell nuclear enlargement, as shown by the small bright nuclei similar to controls (a and c). Thus, nuclear size paralleled changes seen in soma size shown in Fig. 2. (Scale bar: 10 m
m.)
