Strain/plasmid

Genotype/description

Source/ref.

M. tuberculosis

Erdman

Wild-type

W. R. Jacobs, Jr. (Albert Einstein College of Medicine, Bronx, NY)

SSM1

Erdman snm1::Tn5370, Hyg^R

This study

SSM2

Erdman snm2::Tn5370, Hyg^R

This study

SSM3

Erdman snm4::Tn5370, Hyg^R

This study

SSM5

SSM3 + pjsc454, Hyg^R Km^R

This study

SSM6

Erdman Δesat6, Hyg^R

This study

SSM7

Erdman Δcfp10, Hyg^R

This study

Saccharomyces cerevisiae

W303-1a

MATa; ura3-1; leu2-3,-112; his3-11,-15; trp1-1; ade2-1; can1-100

P. Walter (University of California, San Francisco)

EGY48

MATα; trp1, his3, ura3, lexAop-LEU2

R. Brent

Plasmids

PEG202

LexA bait plasmid, HIS3, 2μm

1

PWH1

pEG202 + esat6

This study

PWH3

pEG202 + cfp10

This study

pjsc538

pEG202 + snm1 soluble domain (a.a. 252-747)

This study

pjsc540

pEG202 + snm2

This study

pJG4-5

GAL1prom-GAL4_AD, TRP1, 2μm

1

pjsc401

pJG4-5 + ClaI linker in MCS

This study

PWH2

pjsc401 + esat6

This study

pjsc497

pjsc401 + cfp10

This study

PKW1

pjsc401 + snm2 C-term. domain (a.a. 248-591)

This study

pjsc541

pjsc401 + snm2

This study

pjsc542

pjsc401 + snm2 N-term. domain (a.a. 1-241)

This study

pjsc539

pjsc401 + snm1 soluble domain

This study

pSH18-34

8 x lexAop-lacZ reporter, URA3, 2μm

1

pmv306.kan

int, oriE, Km^R

W. R. Jacobs, Jr. (Albert Einstein College of Medicine, Bronx, NY)

cjsc49

cosmid containing RD1 locus from Erdman

This study

pjsc450

pMV306.kan + Rv3876-Rv3877 from cjsc49

This study

pjsc454

pjsc450, ΔRv3876

This study

pjsc407

oriE, cosA, Hyg^R

This study

pSS4

pjsc407 + Δcfp10 construct

This study

pSS8

pjsc407 + Δesat6 construct

This study