|
Table 2. Oligonucleotide PCR primers used in this study
Gene region |
Plasmid |
5' oligonucleotide (5'-3') |
3' oligonucleotide (5'-3') |
esat6 |
pWH1/2 |
oWH1, GGAATTCATGACAGAGCAGCAGTGG |
oWH2, CCGCTCGAGCTATGCGAACATCCCAGT |
cfp10 |
pjsc497 |
oWH3, GGAATTCATGGCAGAGATGAAGACC |
oWH4, CCATCGATTCAGAAGCCCATTTGCGA |
cfp10 |
pWH3 |
oWH3, GGAATTCATGGCAGAGATGAAGACC |
oWH5, CGGGATCCTCAGAAGCCCATTTGCGA |
snm1 -SD |
pjsc538/9 |
ojsc167, ACATATGGACCTGCTGCAGATC |
ojsc168, AGGATCCCGGCGCGGGTCACG |
snm2 |
pjsc540/1 |
omf43, CCATATGACTGCTGAACCGGAAG |
ojsc166, TCTCGAGTTAACCGGCGCTTGG |
cfp10- 5' |
pSS4 |
oss15, ACTAGTCCGCGGTTAACACGCTT |
oss16, CAAGCTTCGGTCTTCATCTCTGCCAT |
cfp10-3 ' |
pSS4 |
oss17, GTCTAGAGGGCTTCTGACCCGCT |
oss18, TGGTACCGCGATCGGCATCGGAGT |
esat6-5 ' |
pSS8 |
oss19, GACTAGTGGTGATGCCGGCGGCTG |
oss20, GAAGCTTTCCACTGCTGCTCTGTCAT |
esat6-3 ' |
pSS8 |
oss21, GTCTAGATGGGATGTTCGCATAGGGC |
oss22, AGGTACCCTCCGGTTGGCGTTTGTG |
β-actin |
|
osr113, GTGGGCCGCTCTAGGCACCAA |
osr114, CTCTTTGATGTCACGCACGATTTC |
IL-12 p40 |
|
osr105, GGAAGCACGGCAGCAGAATA |
osr106, AACTTGAGGGAGAAGTAGGAATGG |
Tumor necrosis factor α |
|
osr111, CATCTTCTCAAAATTCGAGTGACAA |
osr112, TGGGAGTAGACAAGGTACAACCC |