A synthetic host-guest system achieves avidin-biotin affinity by overcoming enthalpy entropy compensation -- Rekharsky et al. 104 (52): 20737 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Rekharsky et al. 10.1073/pnas.0706407105.

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SI Figure 6

Fig. 6. Competition experiments on complexation of 3 with CB[7]: (a) interaction of 3 (4.5 mM) with CB[7] (0.43 mM) in the solution of 66 mM tetraamine [NH₂(CH₂)₂NH(CH₂)₆NH(CH₂)₂NH₂ 4HCl]. (b) Repetition of experiment on complexation of 3 (4.5 mM) with CB[7] (0.43 mM) in the solution of 66 mM tetraamine [NH₂(CH₂)₂NH(CH₂)₆NH(CH₂)₂NH₂ 4HCl]. (c) Interaction of 3 (4.7 mM) with CB[7] (0.43 mM) in the solution of 73 mM aminomethylcyclohexane HCl (curve fitting was performed using the Single Set of Identical Sites Model).

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Fig. 7. Competition experiments on complexation of 3 with CB[7]: (a) interaction of 3 (4.5 mM) with CB[7] (0.43 mM) in the solution of 66 mM tetraamine [NH₂(CH₂)₂NH(CH₂)₆NH(CH₂)₂NH₂ 4HCl]. (b) repetition of experiment on complexation of 3 (4.5 mM) with CB[7] (0.43 mM) in the solution of 66 mM tetraamine [NH₂(CH₂)₂NH(CH₂)₆NH(CH₂)₂NH₂ 4HCl]. (c) interaction of 3 (4.7 mM) with CB[7] (0.43 mM) in the solution of 73 mM aminomethylcyclohexane HCl (curve fitting was performed using the Competitive Binding Model).

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Fig. 8. Heat effect upon interaction of CB[7] with 3 in H₂O.

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Fig. 9. Competition experiments on complexation of 2 with CB[7] (curve fitting was performed using the Competitive Binding Model).

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Fig. 10. Heat effect upon interaction of CB[7] with 2 in H₂O.

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Fig. 11. Competition experiments on complexation of 1 with CB[7] (curve fitting was performed using the Competitive Binding Model)

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Fig. 12. Enthalpy changes (DH°) for complexation of 1, 2 and 3 with CB[7] measured at 5, 15, 25, 35, and 45°C.

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Fig. 13. Cyclic voltammetric response on glassy carbon (0.071 cm²) of a 1.0 mM solution of guest 3 in 0.1 M NaCl buffered at pH 7 (phosphate) in the absence (discontinuous line) and in the presence (continuous line) of 1.1 equiv of CB[7]. Scan rate: 0.1 V/s.