First assay

Defective

Active

Second assay

Defective

Not expressed

Active

Domain 1

I210, K225, T240, K245

D177, D179, D181, G182, I183, D185, E188, G191, Y192, R200, F202, W206, Y219, S221, P223, W226, S227, T228, A229, D231, Y233, S234, D235, F236, K238, G241, D244, H253

T169, S170, G172, P184, L187, V189, E190, D195, K197, T201, L203, I207, H211, K214, G215, S222, S230, P232, R242, V247, A251, R252

S168, A171, P173, T174, V175, P176, R178, N180, S186, T193, V194, V196, N198, K199, S204, P205, S208, N209, E212, K213, L216, T217, K218, K220, E224, E237, V239, I243, N246, S248, P249, E250

Domain 2

S337, G342, W346, T357, I364, P379, T380, S382, T390, T393, K397, N399, Y411, I419, A420, N422, F427, S428, D451, D453, V455, N458

P254, Y259, P260, V264, I270, K273, H304, I316, G318, I334, L338, R344, E348, N353, R359, N361, N363, R365, Y366, V367, N368, G370, P373, Y375, V384, L391, I394, K395, A396, Q400, S402, Q403, L405, P407, P412, P418, Q424, D425, D426, I432, T433, N435, Q447, Q454, G457, A460, W477, E479, I484

L255, A257, A258, I269, S272, N274, T279, N281, T282, S284, T286, I289, S290, H310, A311, S312, F313, F314, G317, S319, A347, A355, L383, Q389, A406, A417, L421, A423, S429, P431, M434, R468, V469, R470, V471, G474, S475, N476, S478, Q483

V256, I261, V262, H263, D265, M266, E267, N268, L271, E275, D276, Q277, S278, Q280, D283, E285, R287, T288, K291, N292, T293, S294, T295, S296, R297, T298, H299, T300, S301, E302, V303, G305, N306, A307, E308, V309, D315, V320, S321, A322, G323, F324, S325, N326, S327, N328, S329, S330, T331, V332, A333, D335, H336, S339, L340, A341, E343, T345, T349, M350, G351, L352, T354, D356, A358, L360, A362, T369, T371, A372, I374, N376, V377, L378, T381, L385, G386, K387, N388, A392, E398, L401, I404, N408, N409, Y410, S413, K414, N415, L416, T430, Y436, N437, Q438, F439, L440, E441, L442, E443, K444, T445, K446, L448, R449, L450, T452, Y456, I459, T461, Y462, N463, F464, E465, N466, G467, D472, T473, V480, L481, P482, Q485, E486, T487

Domain 3

E515

T488, R503, T522, L523, D546, I547, Q560, N573, Y575, V577, L590, R592

A489, E502, A506, P510, D512, P513, L514, T517, K518, P519, Q541, K563, A585, K586, D593

R490, I491, I492, F493, N494, G495, K496, D497, L498, N499, L500, V501, R504, I505, A507, V508, N509, S511, T516, D520, M521, K524, E525, A526, L527, K528, I529, A530, F531, G532, F533, N534, E535, P536, N537, G538, N539, L540, Y542, Q543, G544, K545, T548, E549, F550, D551, F552, N553, F554, D555, Q556, Q557, T558, S559, N561, I562, N564, Q565, L566, A567, E568, L569, N570, A571, T572, I574, T576, L578, D579, K580, I581, K582, L583, N584, M587, N588, I589, I591, K594, R595

Domain 4

I656, N657, I665, N682, D683, L687

F596, Y598, G606, Y642, G651, L668, I676, K680, Y694, Y699, E704, I724

A607, E609, V612, K613, V619, I620, D632, E645, L652, V655, R659, Y660, Q670, K673, K679, Y681, P686, A700, V701, K703, N705, I708, P710, I726, F727, G735

H597, D599, R600, N601, N602, I603, A604, V605, D608, S610, V611, E614, A615, H616, R617, E618, N621, S622, S623, T624, E625, G626, L627, L628, L629, N630, I631, K633, D634, I635, R636, K637, I638, L639, S640, G641, I643, V644, I646, E647, D648, T649, E650, K653, E654, D658, D661, M662, L663, N664, S666, S667, R669, D671, G672, T674, F675, D677, F678, K684, L685, Y688, I689, S690, N691, P692, N693, K695, V696, N697, V698, T702, T706, I707, N709, S711, E712, N713, G714, D715, T716, S717, T718, N719, G720, I721, K722, K723, L725, S728, K729, K730, G731, Y732, E733, I734