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Fig. 4. PCR (lanes 1–3) and Southern analyses (lanes 4–6) of the DNAs of the deletion (DUL32) and rescued (rescued-UL32) mutant and Towne_BAC that were isolated from E. coli (lanes 1–3) and human fibroblasts (lanes 4–6). In A, PCR products were separated on 1% agarose gels and visualized using ethidium bromide staining. In B, DNAs were digested with HindIII, separated on 0.8% agarose gels, transferred to membranes, and hybridized with a ³²P-labeled probe containing both the KanMX4 and HCMV UL32 sequences. The numbers represent the size of either the PCR DNA products (PCR) or the DNA fragments (HindIII) of BAC-DNAs that were digested with HindIII and hybridized to the radiolabeled probe in Southern analysis.