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Supporting Figure 6

Fig. 6. Sensitivity and precision profile of the reverse-phase protein microarrays. Shown is the difference in log2 ratio intensities between MDA-MB435 and its Erb-B2 transfectant, MDA-MB435, as a function of dilution for 51 of the 52 proteins (excluding Erb-B2 itself) tested in the present study. The differences across the dilution series are small and quite uniform. The diamonds indicate 95% confidence intervals for the mean at each dilution, and the line at the middle of the diamond is the mean itself, as in Fig. 5. The first dilution is omitted because it has a larger error and is not used directly in the analysis.