Institution: NIH Library Sign In as Member / Individual

This Article Full Text Services Alert me to new issues of the journal Request Copyright Permission

Fig. 8. MCMV infection does not destabilize IFNγ-induced transcripts or effect phosphophorylation of STAT1 at Y701 or S727. (a) CIITA and IRF-1 mRNA levels by qRT-PCR 0 or 24 h after MCMV infection (multiplicity of infection = 5) and 6 h after IFNγ treatment. The times indicated are after addition of actinomycin D at 200 mg/ml. (b) Western analysis of phospho-STAT1-Y701, phospho-STAT1-S727, and STAT1 (one of two similar experiments). Lanes are: 1, mock; 2, 100 units/ml IFNγ for 30 min; 3, 1 h postinfection with MCMV followed by 100 units/ml IFNγ for 30 min; 4, 18 hpi with MCMV followed by 100 units/ml IFNγ for 30 min. Lanes 5 and 6 were loaded similarly to lanes 3 and 4 with an independent viral stock. Antibodies used for this experiment were 1:1,000 dilutions of anti-α-actin mAb AC-74 (Sigma), anti-phospho-S727 STAT1 (Upstate Biotechnology, Waltham, MA), and anti-phospho-Y701 STAT1 antibody #9171 or anti-STAT1 #9H2 (Cell Signaling Technology, Beverly, MA).