| This Article | |
 | |
 | Full Text |
| |
| Services | |
| |
| Alert me to new issues of the journal |
| Request Copyright Permission |
| ||||||||||||||||
Table 5. Twitching motility-defective mutants
|
Gene |
Proportion of insertions detected |
|||
|
Within ORF |
Upstream of ORF* |
Total |
||
|
Previously identified genes |
||||
|
pilA |
0.83 (5 of 6) |
0.67 (2 of 3) |
0.78 (7 of 9) |
|
|
pilB |
0.45 (19 of 42) |
0.67 (2 of 3) |
0.47 (21 of 45) |
|
|
pilC |
0.43 (3 of 7) |
0 (0 of 1) |
0.38 (3 of 8) |
|
|
pilD |
0.43 (3 of 7) |
None |
0.43 (3 of 7) |
|
|
pilE |
0.83 (5 of 6) |
None |
0.83 (5 of 6) |
|
|
pilF |
1.0 (1 of 1) |
None |
1.0 (1 of 1) |
|
|
pilG |
None |
0.5 (1 of 2) |
0.5 (1 of 2) |
|
|
pilH |
0.5 (1 of 2) |
None |
0.5 (1 of 2) |
|
|
pilI |
1.0 (1 of 1) |
None |
1.0 (1 of 1) |
|
|
pilJ |
0.57 (12 of 21) |
None |
0.57 (12 of 21) |
|
|
pilK |
0.5 (4 of 8) |
None |
0.50 (4 of 9) |
|
|
pilL/chpA |
0.5 (14 of 30) |
0 (0 of 1) |
0.48 (15 of 31) |
|
|
pilM |
0.42 (5 of 12) |
None |
0.42 (5 of 12) |
|
|
pilN |
0.39 (7 of 18) |
None |
0.39 (7 of 18) |
|
|
pilO |
0.33 (5 of 15) |
None |
0.33 (5 of 15) |
|
|
pilP |
0.5 (5 of 10) |
None |
0.5 (5 of 10) |
|
|
pilQ |
0.57 (17 of 30) |
0 (0 of 2) |
0.53 (17 of 32) |
|
|
pilR |
0.43 (3 of 7) |
None |
0.43 (3 of 7) |
|
|
pilS |
0.21 (3 of 14) |
0.5 (1 of 2) |
0.25 (4 of 16) |
|
|
pilT |
0.11 (1 of 9) |
1.0 (1 of 1) |
0.2 (2 of 10) |
|
|
pilU |
0.83 (5 of 6) |
1.0 (1 of 1) |
0.86 (6 of 7) |
|
|
pilV |
0.57 (4 of 7) |
None |
0.57 (4 of 7) |
|
|
pilW |
0.53 (8 of 15) |
None |
0.53 (8 of 15) |
|
|
pilX |
1.0 (4 of 4) |
None |
1.0 (4 of 4) |
|
|
pilY1 |
0.58 (18 of 31) |
None |
0.58 (18 of 31) |
|
|
pilY2 |
0.33 (1 of 3) |
None |
0.33 (1 of 3) |
|
|
pilZ |
None |
None |
None ‡ |
|
|
algZ/fimS |
0.53 (16 of 30) |
None |
0.53 (16 of 30) |
|
|
algR |
0.83 (5 of 6) |
None |
0.83 (5 of 6) |
|
|
fimT† |
0 (0 of 3) |
None |
0 (0 of 3) |
|
|
fimU |
0.14 (1 of 7) |
1.0 (2 of 2) |
0.33 (3 of 9) |
|
|
fimV |
0.62 (16 of 26) |
None |
0.62 (16 of 26) |
|
|
chpB† |
0 (0 of 5) |
None |
0 (0 of 5) |
|
|
chpC† |
0 (0 of 2) |
None |
0 (0 of 2) |
|
|
chpD† |
0 (0 of 4) |
None |
0 (0 of 4) |
|
|
chpE† |
0 (0 of 3) |
0 (0 of 1) |
0 (0 of 5) |
|
|
vfr |
0.45 (5 of 11) |
0.25 (1 of 4) |
0.4 (6 of 15) |
|
|
rpoN |
0.33 (1 of 3) |
None |
0.33 (1 of 3) |
|
|
dsbA |
0.50 (3 of 6) |
0.5 (1 of 2) |
0.5 (4 of 8) |
|
|
New genes |
||||
|
PA3052 |
0.86 (6 of 7) |
None |
0.86 (6 of 7) |
|
|
PA5330 |
0.67 (2 of 3) |
0 (0 of 1) |
0.5 (2 of 4) |
|
|
glpR |
0.67 (2 of 3) |
None |
0.67 (2 of 3) |
|
|
surE |
0.67 (2 of 3) |
None |
0.67 (2 of 3) |
|
|
PA3217 |
0.63 (5 of 8) |
None |
0.63 (5 of 8) |
|
|
PA4851 |
0.5 (5 of 10) |
None |
0.5 (5 of 10) |
|
|
PA1850 |
0.5 (3 of 6) |
0.5 (1 of 2) |
0.5 (4 of 8) |
|
|
PA0406 |
0.75 (3 of 4) |
0 of 0 |
0.75 (3 of 4) |
|
|
PA03385 |
0.5 (2 of 4) |
1.0 (1 of 1) |
0.6 (3 of 5) |
|
|
PA4916 |
0.67 (2 of 3) |
None |
0.67 (2 of 3) |
|
|
flhB |
0.57 (4 of 7) |
None |
0.57 (4 of 7) |
|
|
fimL |
0.5 (3 of 6) |
None |
0.5 (3 of 6) |
|
|
ycjC |
0.67 (2 of 3) |
None |
0.67 (2 of 3) |
|
|
PA0716-0717§ |
None |
0.75 (3 of 4) |
0.75 (3 of 4) |
|
|
PA5321-5322§ |
None |
0.5 (3 of 6) |
0.5 (3 of 6) |
|
The proportion of insertions in genes required for twitching motility is presented. Mutants were scored as defective if they exhibited a sharp colony edge on LB agar. The "previously identified" group of genes is associated with twitching by genetic and other criteria (reviewed in refs. 1 and 2). The "new genes" group corresponds to genes identified at least twice in our screens in which ≥50% of alleles were scored as twitching-defective. One of the new genes (PA0406) was independently identified in a recent study (3). A total of 1.7% of the mutants in the collection was scored as twitching-defective.
*Insertions situated in intergenic sequence <250 bp upstream of the initiator codon.
†
FimT mutants wre previously reported to be twitching-proficient (4), whereas chpB-chpE mutant phenotypes have not been described.‡
An insertion near the 3' end of a gene seemingly unrelated to motility (encoding a DNA polymerase holoenzyme subunit) immediately upstream of pilZ caused a nontwitching phenotype, perhaps because of a polar effect on pilZ expression.§
These two intergenic spaces produced twitchless phenotypes three out of three times when they were hit
1. Mattick, J. W. (2002) Annu. Rev. Microbiol. 56, 289-314.
2. Ha, U-H., Wang, Y. & Jin, S. (2003) Infect. Immun. 71, 1590-1595.
3. D'Argenio, D. A., Gallagher, L. A. & Manoil, C. (2001) J. Bacteriol. 183, 1466-1471.
4. Alm, R. & Mattick, J. W. (1996) J. Bacteriol. 178, 3809-3817.