|
Table 3. Oligonucleotides used in this study
Primer |
Restriction site |
Sequence (5’ to 3’) |
Corresponding gene |
BbF |
Bam HI/NdeI |
CGCGGATCCATATGTGAAAACAGCACACTGGGC |
(Bb) 699964-699947 |
BbR |
Bam HI/NdeI |
CGCGGATCCATATGGGCTTAAACGTATTTGCTTG |
(Bb) 698397-698415 |
7R/2 |
Nde I |
CCTTAAGCATATGTATACCTCCATTACAGATATTG |
(Bs) 88724-88699 |
7F/5 |
Bam HI |
CGGGATCCCAGTGAAAATGAGAATGGGCTGG |
(Bs) 88046-88069 |
12F |
|
TGTCAGAAGAAATGAATGACC |
(Sa) 547572-547592 |
12R |
|
TTATTTTTGTCTCATATAAGGG |
(Sa) 549058-549037 |
14F |
Nco I |
CCGATAGACGAAGAGGACCATGGTGAGCTC |
(Bb) 547522-547551 |
14R |
Xma I |
GGTCATAAAATGACTCCCGGGCTC |
(Bb) 547635-547612 |
23 |
|
TACGACAAACGCCTTAAAAGGGCGCT |
(Bb) 547567-547592 |
24 |
|
TTCGACCCTCTGATTAAAAGTCAGAT |
(Bs) 3172604-3172629 |
RpsF/1 |
Bam HI |
CGGGATCCGGCGGATAATTGATCTTTAG |
(Bs) 3034442-3034462 |
RpsR/1 |
Nde I |
GGAATTCCATATGATGACTCCTCCTTTGG |
(Bs) 3034772-3034750 |
RpsF/2 |
Nd eI |
GGAATTCCATATGAAAAACCCCTGCCGCTATG |
(Bs) 3035391-3035410 |
RpsR/2.2 |
Bam HI |
CGGGATCCATGGAAGTTGACAGCACCC |
(Bs) 3035861-3035842 |