Fig. 5. Transient NADPH depletion and oxidized glutathione (GSSG) accumulation upon a 10-fold pulse of oxidative load lasting 10 s for normal glucose 6-phosphate dehydrogenase (G6PD) and glutathione reductase (GSR) activities (unbroken line), 10% residual G6PD activity (dashed line), and 10% residual GSR activity (dotted line). The thin horizontal line segment indicates the schedule of the pulse. The plots for GSSG at normal and 10% residual G6PD activity nearly overlap. At 10% residual GSR activity, the resting steady-state concentration of GSSG is 10-fold higher than at normal GSR activity.
