Fig. 11. Genomic organization of RAFTIN1 gene family in wheat and rice. (A) Schematic representation of the RAFTIN1 genes cloned from wheat (taRAFTIN1a and taRAFTIN1b) and rice (osRAFTIN1). The numbers denote nucleotide sequence length of the untranslated regions (UTR), exons (boxes) and introns (triangles). Image is not drawn to scale. (B) RAFTIN1 gene in rice. Southern blot of rice genomic DNA (≈10 mg) digested with HindIII (H), EcoRI (E), and BamHI (B) and probed with the entire osRAFTIN1 ORF retrieved by PCR; size markers are from a l DNA-HindIII digest. (C) taRAFTIN1a and closely related sequences in hexapoid (Triticum aestivum, AABBDD), tetraploid (Triticum turgidum, AABB) and diploid wheats (Triticum urartu, AA; Triticum tauschii, DD), and diploid rice (Oryza sativa). Southern blot of genomic DNA (≈15 mg for wheat and ≈10 mg for rice) restricted with HindIII (H) and EcoRI (E) and probed with the entire taRAFTIN1a ORF retrieved by PCR; size markers are from a l DNA-HindIII digest. The two bands in the T. tauschii HindIII digest were likely due to an internal HindIII in the gene. The band sizes (HindIII, 7.1 kb; EcoRI, 4.4 kb; BamHI, 6.9 kb) in rice agreed with expected sizes from genome sequence (GenBank accession no: AP000364).
