Files in this Data Supplement:
Fig. S1. Overview of the 3D pdf as viewed in Acrobat. The myriad of panels (purple, alphabetized) and tools (black, numbered) are: (a) Acrobat toolbar; (b) Acrobat advanced editing tools (not available in Adobe Reader); (c) opened document; (d) 3D object embedded in the document; (e) 3D toolbar to edit or interact with 3D object (Adobe Reader does not have the edit tools); (f) custom panel within the document containing custom buttons created with Acrobat and Illustrator as described in the advanced segment; (g) model tree navigation panel to interact with the 3D object; (1) select tool; (2) select object tool; (3) button tool; (4) touchup object tool; (5) add 3D comment button; (6, 7) show model tree button; (8) create view button.
Fig. S2. Basic 3D pdf of an E11.5 mouse embryonic heart. This 3D pdf file is preferably viewed in Adobe Reader 9.3 or higher. To rotate, click and hold the left mouse button and move the mouse; to zoom, click and hold the right mouse button and move the mouse; to translate, click and hold the left and right mouse buttons and move the mouse.
Fig. S3. Advanced 3D pdf of an E11.5 mouse embryonic heart. This 3D pdf file is preferably viewed in Adobe Reader 9.3 or higher. Javascript must be enabled.
Preferred settings for Adobe Reader 9.3:
In the menu select: Edit\Preferences to ensure the following:
(1) In JavaScript: under JavaScript
enable 'Enable Acrobat JavaScript'
(2) In 3D & Multimedia: under 3D Tool Options
for 'Open Model Tree on 3D Activation' choose 'Use Annotation�s Settings'
for 'Default Toolbar State' choose 'Use Annotation�s Settings'
disable 'Show 3D orientation axis'
(3) In 3D & Multimedia: under Auto-Degrade Options
for the 'Optimization Scheme for Low Framerate' select 'None'.
How to use this 3D pdf file. On the top left are the preset view buttons to switch the 3D model into a predefined view. Below the buttons for preset views there is a list of the structures (myocardium etc.) with three buttons per structure to switch it on or off or make it transparent.
To further interact with the 3D model. To rotate, click and hold the left mouse button and move the mouse; to zoom, click and hold the right mouse button and move the mouse; to translate, click and hold the left and right mouse buttons and move the mouse.