Supplementary material for Cunningham and Gantt (February 20, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.051618398
Supplemental Figure 8Fig. 8.
Alignment of deduced amino acid sequences of plant b- and e-cyclases, neoxanthin synthases (NSY), and capsanthin capsorubin synthase (CCS) enzymes, and a cyanobacterial (Synechococcus PCC7942) b-cyclase. This alignment was used to produce a neighbor-joining tree with the Synechococcus sequence used as the outgroup. Residues that align prior to the initial residue of the Synechococcus b-cyclase are not shown and were not used in the calculation of distances. Alignment positions with a gap were also excluded from the analysis. Residues are in white text on a black background where 100% of the amino acids in that position are identical. Residues are in black text on a dark grey background where 75% or more are identical and in black text on a light grey background where greater than 50% are identical. The initial alignment was created by using CLUSTAL X version 1.8 (1). GenBank accession numbers are as follows. For LCYe: Adonis LCYe-1 (AaE1), AF321535; Adonis LCYe-2 (AaE2), AF321536; Arabidopsis (AtE), U50738; lettuce (LsE), AF321538; marigold (TeE), AF251016; potato (StE), AF321537; tomato (LeE), Y14387. For LCYb: Adonis (AaB), AF321534; Arabidopsis (AtB), U50739; marigold (TeB), AF251017; Narcissus (NpB), X98796; pepper (CaB), X86221; tobacco (NtB), X81787; tomato (LeB), X86452; Synechococcus (SynB), X74599. For NSY: Citrus (C. sinensis; CsNSY), AF169241; potato (StNSY), AJ272136; tomato (LeNSY), Y18297. For CCS: pepper (CaCCS), X76165.References:
1. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., & Higgins, D.G. (1997) Nucleic Acid Res. 25, 4876-4882.