Supplementary material for Grünenfelder et al. (April 3, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.071538098.
Supplemental Figure 5Fig. 5.
(A) High-resolution two-dimensional (2-D) gel autoradiogram of a pulse-labeled exponentially growing Caulobacter crescentus culture. The numbers on the horizontal axis of the gel indicate the isoelectric point and the numbers on the vertical axis of the gel indicate the molecular weight (´ 10–3). (B) The positions of 144 protein spots with differential cell cycle expression patterns are indicated on the 2-D gel autoradiogram shown in A. The arrows point to the spot positions, and the black boxes indicate the corresponding 2-D gel spot (SSP; see Table 2) numbers. The spots shown in each image belong to the expression clusters indicated in the heading. (C) The positions of 154 protein spots with expression induced or repressed by the synchronization procedure are indicated on the 2-D gel autoradiogram shown in A. The arrows point to the spot positions, and the black boxes indicate the corresponding 2-D gel spot (SSP; see Table 2) numbers. All spots shown in each image are either induced or repressed by the synchronization procedure, as indicated in the heading. (D) The positions of 72 protein spots that decrease or increase in intensity during the chase period are indicated on the 2-D gel autoradiogram shown in A. The arrows point to the spot positions, and the black boxes indicate the corresponding 2-D gel spot (SSP; see Table 2) numbers. All spots shown in one image are either decreasing or increasing during chase, as indicated in the heading.