Just-in-Time Control of Spo0A Synthesis in Bacillus subtilis by Multiple Regulatory Mechanisms

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Table S1, B. subtilis strains used in this study; Table S2, plasmids used in this study; Table S3, oligonucleotides used in this study; Fig. S1, immunoblot analysis of Spo0A accumulation; Fig. S2, mutagenesis of a P_ς-lacZ translational reporter fusion reveals four categories of mutants; Fig. S3, mutations in O₁ and O₃ impair Spo0A binding to corresponding sites; Fig. S4, construction of a chimeric promoter between P_ς and P_spoVG; Fig. S5, a mutant lacking P_v is slightly inhibited for sporulation and impaired for DNA-mediated transformation
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