The Role of CYP3A4 mRNA Transcript with Shortened 3′-Untranslated Region in Hepatocyte Differentiation, Liver Development, and Response to Drug Induction

Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1 -

  Slope and R2 of standard curves and PCR efficiency

• Supplemental Table 6 -

  MIQE checklist

• Supplemental Figure 1 -

  Design and validation of primer sets for quantification of the CYP3A4 mRNA transcripts by real-time PCR

• Supplemental Figure 2a -

  Sequencing results of the shorter 3'-UTR transcript from the 3�RACE-PCR product

• Supplemental Figure 2b -

  Alignment of the sequence of the 3'RACE-PCR product to human genome with perfect match to the shorter 3'-UTR of the CYP3A4 gene.

• Supplemental Figure 3 -

  Validation of transfection plasmids inserted with different CYP3A4 3'-UTR sequences into pMirTarget vector (7.9 kb) by gel images

• Supplemental Figure 4 -

  Transfection efficiency (mean: 14.2%; 95% CI: 11.7% -16.7%) measured by red fluorescence protein (RFP) expression with CYP3A4 3�-UTR pMirTarget plasmids