Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Fig. S1, DNA runoff sequencing to determine BceSI cut site in pBR322; Fig. S2, gene organization of the BceSII R-M system and restriction activity assay for BceSII; Fig. S3, gene organization of the BceSIII R-M system, SDS-PAGE analysis of partially purified BceSIII, and digestion of pBR322 by BceSIII; Fig. S4, phylogenetic analysis of four groups of PLD family endonucleases with the signature catalytic site HxKxD; Fig. S5, ATPase and GTPase activity assays for BceSIV endonuclease; Fig. S6, DNA nicking activity assay for ParB-M fusion protein, and resistance to restriction digestion by M.BceSV modification; Fig. S7, SDS-PAGE analysis of Bce14579I R1, R1/R2, and IPTG-induced cell extracts, and digestion of λ DNA by partially purified R1 and R1/R2 enzymes
PDF file, 5.1MB. - Supplemental file 2 - Legends to Fig. S1 to S7
MS Word document, 736K. - Supplemental file 3 - Table S1, predicted type II and III R and M genes in sequenced B. cereus genomes; Table S2, predicted R and M genes in sequenced genomes of Bacillus anthracis and Bacillus thuringiensis strains
MS Word document, 119K.