Heterologous Expression of Polycyclic Aromatic Hydrocarbon Ring-Hydroxylating Dioxygenase Genes from a Novel Pyrene-Degrading Betaproteobacterium

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Contig size versus G+C% for the metagenomic library (Fig. S1), neighbor-joining 16S rRNA gene tree showing betaproteobacterial sequences with similarity to the PG1 sequence recovered from the metagenomic library (Fig. S2), primers used during this study (Table S1), mismatches of PG1 RHD alpha-subunit nucleotide sequences to recommended PCR primer pairs (Table S2), and proteins from GenBank with highest similarity to predicted RHD protein sequences and other ORFs (Table S3).
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