New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based Counterselection System for Generating Mutants by Allelic Replacement in Staphylococcus aureus

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Detailed protocol for using the pRLY vectors (Protocol S1).
  PDF file, 95K.
• Supplemental file 2 - Detailed protocol for using pRLSAYx9 vectors (Protocol S2).
  PDF file, 64K.
• Supplemental file 3 - Detailed protocol for using the pRLBxR8 and pRLBxR9 vectors (Protocol S3).
  PDF file, 74K.
• Supplemental file 4 - Summary of all the final pRLY vectors described in the text (Fig. S1).
  PDF file, 1.3 MB.
• Supplemental file 5 - Thermosensitivity of the pRLYx8 and pRLYx9 vectors (Fig. S2).
  PDF file, 1.1 MB.
• Supplemental file 6 - Summary of all the final pRLB vectors described in the text (Fig. S3).
  PDF file, 1 MB.
• Supplemental file 7 - Schematic drawing for primers and PCR products used for generating and verifying mutants by allelic replacement (Fig. S4).
  PDF file, 327K.
• Supplemental file 8 - Primers used in this study (Table S1).
  PDF file, 78K.