• Video 1 Atg9 vesicles were highly mobile in the cytoplasm.
• Video 2 The motion of Atg9 vesicles was not altered after the treatment with latrunculin A.
• Video 3 Accumulation of Atg9 at the Golgi apparatus in cells depleted of Sec7.
• Video 4 Accumulation of Atg9 at the Golgi apparatus in sec7ts cells.
• Video 5 Mislocalization of Atg9 in atg23Δ or atg27Δ cells.
• Video 6 Aberrant accumulation of Atg9 in cells overexpressing Atg9.
• Video 7 In response to starvation, cytoplasmic Atg9 vesicles assembled to the PAS.
• Video 8 Atg9 vesicles assembled individually to the PAS.
• Video 9 Atg9 localized onto the autophagosomal membranes.
• Video 10 The intensity of Atg9-2×GFP clusters on the autophagosomal membrane was apparently comparable to that of the cytoplasmic mobile Atg9-2×GFP vesicles.