Published July 23, 2012 // JCB vol. 198 no. 2 219-233
The Rockefeller University Press, doi: 10.1083/jcb.201202061

Atg9 vesicles are an important membrane source during early steps of autophagosome formation

Video 1 Atg9 vesicles were highly mobile in the cytoplasm.

Video 2 The motion of Atg9 vesicles was not altered after the treatment with latrunculin A.

Video 3 Accumulation of Atg9 at the Golgi apparatus in cells depleted of Sec7.

Video 4 Accumulation of Atg9 at the Golgi apparatus in sec7ts cells.

Video 5 Mislocalization of Atg9 in atg23Δ or atg27Δ cells.

Video 6 Aberrant accumulation of Atg9 in cells overexpressing Atg9.

Video 7 In response to starvation, cytoplasmic Atg9 vesicles assembled to the PAS.

Video 8 Atg9 vesicles assembled individually to the PAS.

Video 9 Atg9 localized onto the autophagosomal membranes.

Video 10 The intensity of Atg9-2×GFP clusters on the autophagosomal membrane was apparently comparable to that of the cytoplasmic mobile Atg9-2×GFP vesicles.

Supplemental material (PDF)