Analyses of Dynein Heavy Chain Mutations Reveal Complex Interactions Between Dynein Motor Domains and Cellular Dynein Functions
Supporting Information for Sivagurunathan et al, 2012
Supporting Information
- Supporting Information - Figures S1-S5, Files S1-S9, and Tables S1-S3 (PDF, 9.8 MB)
- Figure S1 - Generation of DIC-mCherry expressing N.crassa strain (PDF, 1.7 MB)
- Figure S2 - Protein levels in representative DHC mutant strains (PDF, 472 KB)
- Figure S3 - An 8-second time series showing photobleaching of bright hyphal tip dynein fluorescence in a wildtype strain (PDF, 359 KB)
- Figure S4 - Colocalization of dynein and dynactin in wildtype and DHC mutant strains (PDF, 7.1 MB)
- Figure S5 - A. Dynein purification scheme for N.crassa. B. Representative silver-stained gel image showing the final elution fraction from the StrepTrap column (PDF, 244 KB)
- Table S1 - Summary of vesicle transport dynamics (PDF, 52 KB)
- Table S2 - Summary of dynein mislocalization phenotypes (PDF, 49 KB)
- Table S3 - Primary Neurospora crassa strains used in this study (PDF, 66 KB)
- File S1 - Representative live cell movies of FM 4-64 labeled vesicle trafficking in hyphal tips from each of the strains analyzed. Each movie was of 30 seconds duration and is sped up six-fold. Each movie is 86 μm x 65 μm in size (.zip, 12.7 MB)
- File S2 - (.zip, 12.6 MB)
- File S3 - (.zip, 12.7 MB)
- File S4 - (.zip, 12.1 MB)
- File S5 - (.zip, 12.2 MB)
- File S6 - (.zip, 12.7 MB)
- File S7 - (.zip, 12.7 MB)
- File S8 - (.zip, 12.6 MB)
- File S9 - (.zip, 12.6 MB)