Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Putative haloalkane dehalogenases from psychrophilic organisms (Table S1), quantitative comparison of activity and specificity of DpcA with the known biochemically characterized HLDs using the principal component analysis (Fig. S1), 30 halogenated substrates used for HLD substrate specificity testing (Table S2), comparison of amino acid composition of DpcA with biochemically characterized HLDs from mesophilic organisms (Table S3), and steady-state kinetics of haloalkane dehalogenase DpcA with 1-bromobutane and 1,3-dibromopropane (Fig. S2).
  PDF file, 466K.