Identification and Biochemical Evidence of a Medium-Chain-Length Polyhydroxyalkanoate Depolymerase in the Bdellovibrio bacteriovorus Predatory Hydrolytic Arsenal

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  Isolated lytic plaques of B. bacteriovorus HD100 growing on P. putida as prey and amino acid alignment (Fig. S1), production of PhaZ_Bd in E. coli (Fig. S2), semipurification of native PhaZ_Bd produced by P. putida AΩ (pIZBd1) (Fig. S3), and identification by HPLC-MS of the mcl PHA hydrolysis products catalyzed by the B. bacteriovorus HD100 depolymerase at 1 h of enzymatic hydrolysis (Fig. S4).

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