Predicted Protein Subcellular Localization in Dominant Surface Ocean Bacterioplankton

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Summary of supplemental material.

  PDF, 10K

• Supplemental file 4 -

  Performance of MetaP (Table S3) and new MetaP (Table S4) in predicting nonmembrane proteins, performance of PSLDOC (Table S5) and CELLO (Table S6) in predicting inner- and outer-membrane proteins, and Pearson's correlation coefficient between subcellular localization and axis coordinates in two-dimensional space (Table S10).

  PDF, 74K

• Supplemental file 7 -

  Data set for genes relevant to phototrophy in the transcriptomes (Data Set 1).

  PDF, 121K

• Supplemental file 8 -

  Training data sets consisting of protein sequences whose subcellular localization had been experimentally verified (Data Set 2).

  PDF, 1.3M

• Supplemental file 2 -

  Protein subcellular localization of major marine bacterial taxa using genomic, metagenomic, and metatranscriptomic sequences (Table S1).

  XLS, 72K

• Supplemental file 3 -

  GOS samples in six geographic locations and assignment of sequences to taxonomic groups based on best BLAST hit (Table S2).

  XLS, 28K

• Supplemental file 5 -

  Numbers of gene transcripts in day vs. night samples binned to COG functional categories for predicted cytoplasmic, inner-membrane, periplasmic, outer-membrane, and extracellular proteins (Table S11).

  XLS, 754K

• Supplemental file 6 -

  Relative change in COG family abundance (normalized by library size) between day and night gene expressions for predicted cytoplasmic, inner-membrane, and periplasmic proteins (Table S12).

  XLS, 532K