Natural Transformation of an Engineered Helicobacter pylori Strain Deficient in Type II Restriction Endonucleases

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Fig. S1, sacB-mediated counterselection used to construct Helicobacter pylori type II restriction endonuclease-deficient mutants
  Fig. S2, growth of H. pylori wild type and the type II restriction endonuclease-deficient mutants
  Fig. S3, survival of H. pylori wild type and the type II restriction endonuclease-deficient mutants after UV exposure
  Fig. S4, spontaneous mutation frequency of H. pylori mutants based on evaluating the frequency of an rpoB point mutation which confers resistance to rifampin
  Table S1, plasmids and bacterial strains
  Table S2, oligonucleotides
  Table S3, susceptibility of H. pylori wild-type and mutant strains to levofloxacin
  PDF file, 591K.