Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie 1. Puncta of full length Baz::GFP show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section and 32 μm wide. The imaging was performed at 4.0 s/frame and is shown at 20 frames/s (80x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.
- Movie02 - Movie 2. Puncta of Baz∆PDZ1-3::GFP do not show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section and 32 μm wide. The imaging was performed at 5.0 s/frame and is shown at 20 frames/s (100x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.
- Movie03 - Movie 3. Puncta of Baz∆PDZ2-3::GFP show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section and 32 μm wide. The imaging was performed at 4.0 s/frame and is shown at 20 frames/s (80x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.
- Movie04 - Movie 4. Puncta of Baz∆PDZ1+3::GFP show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section) and 32 μm wide. The imaging was performed at 4.0 s/frame and is shown at 20 frames/s (80x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.
- Movie05 - Movie 5. Puncta of Baz∆PDZ1-2::GFP show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section and 32 μm wide. The imaging was performed at 4.0 s/frame and is shown at 20 frames/s (80x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.
- Movie06 - Movie 6. Puncta of BazS151A, S1085A::GFP show basal-to-apical translocations during cellularization. Construct expressed maternally with mgv. Each frame is a single confocal section and 32 μm wide. The imaging was performed at 8s/frame and is shown at 10 frames/s (80x real time). The imaging was performed at the side of the embryo for an optical cross section of the forming cells.